The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice
WRKY proteins are a large super family of transcriptional regulators primarily involved in various plant physiological programs. In present study, the expression profile and putative function of the WRKY transcriptional factor, WRKY78, in rice were identified. Real-time RT-PCR analysis showed that O...
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| Veröffentlicht in: | Planta 2011-09, Vol.234 (3), p.541-554 |
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| creator | Zhang, Chang-Quan Xu, Yong Lu, Yan Yu, Heng-Xiu Gu, Ming-Hong Liu, Qiao-Quan |
| description | WRKY proteins are a large super family of transcriptional regulators primarily involved in various plant physiological programs. In present study, the expression profile and putative function of the WRKY transcriptional factor, WRKY78, in rice were identified. Real-time RT-PCR analysis showed that OsWRKY78 transcript was most abundant in elongating stems though its expression was detected in all the tested organs. The expression profiles were further confirmed by using promoter-GUS analysis in transgenic rice. OsWRKY78::GFP fusion gene transient expression analysis demonstrated that OsWRKY78 targeted to the nuclei of onion epidermal cell. Furthermore, OsWRKY78 RNAi and overexpression transgenic rice lines were generated. Transgenic plants with OsWRKY78 overexpression exhibited a phenotype identical to the wild type, whereas inhibition of OsWR KY78 expression resulted in a semi-dwarf and small kernel phenotype due to reduced cell length in transgenic plants. In addition, a T-DNA insertion mutant line oswrky78 was identified and a phenotype similar to that of RNAi plants was also observed. Grain quality analysis data showed no significant differences, with the exception of minor changes in endosperm starch crystal structure in RNAi plants. Taken together, these results suggest that OsWRKY78 may acts as a stem elongation and seed development regulator in rice. |
| doi_str_mv | 10.1007/s00425-011-1423-y |
| format | Article |
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In present study, the expression profile and putative function of the WRKY transcriptional factor, WRKY78, in rice were identified. Real-time RT-PCR analysis showed that OsWRKY78 transcript was most abundant in elongating stems though its expression was detected in all the tested organs. The expression profiles were further confirmed by using promoter-GUS analysis in transgenic rice. OsWRKY78::GFP fusion gene transient expression analysis demonstrated that OsWRKY78 targeted to the nuclei of onion epidermal cell. Furthermore, OsWRKY78 RNAi and overexpression transgenic rice lines were generated. Transgenic plants with OsWRKY78 overexpression exhibited a phenotype identical to the wild type, whereas inhibition of OsWR KY78 expression resulted in a semi-dwarf and small kernel phenotype due to reduced cell length in transgenic plants. In addition, a T-DNA insertion mutant line oswrky78 was identified and a phenotype similar to that of RNAi plants was also observed. Grain quality analysis data showed no significant differences, with the exception of minor changes in endosperm starch crystal structure in RNAi plants. Taken together, these results suggest that OsWRKY78 may acts as a stem elongation and seed development regulator in rice.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-011-1423-y</identifier><identifier>PMID: 21547461</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer</publisher><subject>Agriculture ; Biological and medical sciences ; Biomedical and Life Sciences ; China ; Ecology ; Endosperm - genetics ; Endosperm - growth & development ; Forestry ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genes ; Genetic Variation ; Grains ; Life Sciences ; Molecular Sequence Data ; Original Article ; Oryza - genetics ; Oryza - growth & development ; Panicles ; Phenotypes ; Plant Sciences ; Plant Stems - genetics ; Plant Stems - growth & development ; Plants ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - growth & development ; Reverse transcriptase polymerase chain reaction ; Rice ; Starches ; Transcription factors ; Transcription Factors - genetics ; Transcription, Genetic ; Transgenic plants</subject><ispartof>Planta, 2011-09, Vol.234 (3), p.541-554</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-8b72fb8f62aa8ce28b7ee9967dd7b2c763c1ef3bb560f2a8ec02966283dace6a3</citedby><cites>FETCH-LOGICAL-c488t-8b72fb8f62aa8ce28b7ee9967dd7b2c763c1ef3bb560f2a8ec02966283dace6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23884334$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23884334$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24475979$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21547461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Chang-Quan</creatorcontrib><creatorcontrib>Xu, Yong</creatorcontrib><creatorcontrib>Lu, Yan</creatorcontrib><creatorcontrib>Yu, Heng-Xiu</creatorcontrib><creatorcontrib>Gu, Ming-Hong</creatorcontrib><creatorcontrib>Liu, Qiao-Quan</creatorcontrib><title>The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice</title><title>Planta</title><addtitle>Planta</addtitle><addtitle>Planta</addtitle><description>WRKY proteins are a large super family of transcriptional regulators primarily involved in various plant physiological programs. In present study, the expression profile and putative function of the WRKY transcriptional factor, WRKY78, in rice were identified. Real-time RT-PCR analysis showed that OsWRKY78 transcript was most abundant in elongating stems though its expression was detected in all the tested organs. The expression profiles were further confirmed by using promoter-GUS analysis in transgenic rice. OsWRKY78::GFP fusion gene transient expression analysis demonstrated that OsWRKY78 targeted to the nuclei of onion epidermal cell. Furthermore, OsWRKY78 RNAi and overexpression transgenic rice lines were generated. Transgenic plants with OsWRKY78 overexpression exhibited a phenotype identical to the wild type, whereas inhibition of OsWR KY78 expression resulted in a semi-dwarf and small kernel phenotype due to reduced cell length in transgenic plants. In addition, a T-DNA insertion mutant line oswrky78 was identified and a phenotype similar to that of RNAi plants was also observed. Grain quality analysis data showed no significant differences, with the exception of minor changes in endosperm starch crystal structure in RNAi plants. Taken together, these results suggest that OsWRKY78 may acts as a stem elongation and seed development regulator in rice.</description><subject>Agriculture</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>China</subject><subject>Ecology</subject><subject>Endosperm - genetics</subject><subject>Endosperm - growth & development</subject><subject>Forestry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genetic Variation</subject><subject>Grains</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Original Article</subject><subject>Oryza - genetics</subject><subject>Oryza - growth & development</subject><subject>Panicles</subject><subject>Phenotypes</subject><subject>Plant Sciences</subject><subject>Plant Stems - genetics</subject><subject>Plant Stems - growth & development</subject><subject>Plants</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Reverse transcriptase polymerase chain reaction</subject><subject>Rice</subject><subject>Starches</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic</subject><subject>Transgenic plants</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kV1rFDEUhoNY7Fr9AV4ooSC9Gs3XJJlLKdUWCwWpiBcSMpkz6ywzmTVnprD_vllndcELr_LxPuc9J28IecXZO86YeY-MKVEWjPOCKyGL3ROy4kqKQjBln5IVY3nPKlmekueIG8ayaMwzcip4qYzSfEV-3P8E-u3L5-90Sj5iSN126sZIWx-mMdE73GvG0gTrufcTIMUJBgr9GNf-N-ljQxGgoQ085OvtAHGiXaSpC_CCnLS-R3h5WM_I149X95fXxe3dp5vLD7dFUNZOha2NaGvbauG9DSDyGaCqtGkaU4tgtAwcWlnXpWat8BYCE5XWwsrGB9BenpGLxXebxl8z4OSGDgP0vY8wzuisLUstlJKZPP-H3Ixzinm4PSQqo7XKEF-gkEbEBK3bpm7waec4c_vk3ZK8y8m7ffJul2veHIzneoDmb8WfqDPw9gB4DL5vc9yhwyOnlCkrU2VOLBxmKa4hHSf8X_fXS9EG87cdTaW1-dFKPgIS-KVT</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Zhang, Chang-Quan</creator><creator>Xu, Yong</creator><creator>Lu, Yan</creator><creator>Yu, Heng-Xiu</creator><creator>Gu, Ming-Hong</creator><creator>Liu, Qiao-Quan</creator><general>Springer</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110901</creationdate><title>The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice</title><author>Zhang, Chang-Quan ; Xu, Yong ; Lu, Yan ; Yu, Heng-Xiu ; Gu, Ming-Hong ; Liu, Qiao-Quan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-8b72fb8f62aa8ce28b7ee9967dd7b2c763c1ef3bb560f2a8ec02966283dace6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Agriculture</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>China</topic><topic>Ecology</topic><topic>Endosperm - genetics</topic><topic>Endosperm - growth & development</topic><topic>Forestry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genetic Variation</topic><topic>Grains</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>Original Article</topic><topic>Oryza - genetics</topic><topic>Oryza - growth & development</topic><topic>Panicles</topic><topic>Phenotypes</topic><topic>Plant Sciences</topic><topic>Plant Stems - genetics</topic><topic>Plant Stems - growth & development</topic><topic>Plants</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - growth & development</topic><topic>Reverse transcriptase polymerase chain reaction</topic><topic>Rice</topic><topic>Starches</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription, Genetic</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Chang-Quan</creatorcontrib><creatorcontrib>Xu, Yong</creatorcontrib><creatorcontrib>Lu, Yan</creatorcontrib><creatorcontrib>Yu, Heng-Xiu</creatorcontrib><creatorcontrib>Gu, Ming-Hong</creatorcontrib><creatorcontrib>Liu, Qiao-Quan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Chang-Quan</au><au>Xu, Yong</au><au>Lu, Yan</au><au>Yu, Heng-Xiu</au><au>Gu, Ming-Hong</au><au>Liu, Qiao-Quan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice</atitle><jtitle>Planta</jtitle><stitle>Planta</stitle><addtitle>Planta</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>234</volume><issue>3</issue><spage>541</spage><epage>554</epage><pages>541-554</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>WRKY proteins are a large super family of transcriptional regulators primarily involved in various plant physiological programs. In present study, the expression profile and putative function of the WRKY transcriptional factor, WRKY78, in rice were identified. Real-time RT-PCR analysis showed that OsWRKY78 transcript was most abundant in elongating stems though its expression was detected in all the tested organs. The expression profiles were further confirmed by using promoter-GUS analysis in transgenic rice. OsWRKY78::GFP fusion gene transient expression analysis demonstrated that OsWRKY78 targeted to the nuclei of onion epidermal cell. Furthermore, OsWRKY78 RNAi and overexpression transgenic rice lines were generated. Transgenic plants with OsWRKY78 overexpression exhibited a phenotype identical to the wild type, whereas inhibition of OsWR KY78 expression resulted in a semi-dwarf and small kernel phenotype due to reduced cell length in transgenic plants. In addition, a T-DNA insertion mutant line oswrky78 was identified and a phenotype similar to that of RNAi plants was also observed. Grain quality analysis data showed no significant differences, with the exception of minor changes in endosperm starch crystal structure in RNAi plants. Taken together, these results suggest that OsWRKY78 may acts as a stem elongation and seed development regulator in rice.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer</pub><pmid>21547461</pmid><doi>10.1007/s00425-011-1423-y</doi><tpages>14</tpages></addata></record> |
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| subjects | Agriculture Biological and medical sciences Biomedical and Life Sciences China Ecology Endosperm - genetics Endosperm - growth & development Forestry Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes Genetic Variation Grains Life Sciences Molecular Sequence Data Original Article Oryza - genetics Oryza - growth & development Panicles Phenotypes Plant Sciences Plant Stems - genetics Plant Stems - growth & development Plants Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Reverse transcriptase polymerase chain reaction Rice Starches Transcription factors Transcription Factors - genetics Transcription, Genetic Transgenic plants |
| title | The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice |
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