Effects of Donor Age, Gender, and In Vitro Cellular Aging on the Phenotypic, Functional, and Molecular Characteristics of Mouse Bone Marrow-Derived Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) are a very important adult stem cell population with a multitude of potential applications in regenerative medicine. The thorough characterization of the bone marrow MSC (BM-MSC) population derived from the BALB/c species was essential, considering the significance of t...
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| creator | Katsara, Olga Mahaira, Louisa G. Iliopoulou, Eleni G. Moustaki, Ardiana Antsaklis, Aristidis Loutradis, Dimitrios Stefanidis, Konstantinos Baxevanis, Constantin N. Papamichail, Michael Perez, Sonia A. |
| description | Mesenchymal stem cells (MSCs) are a very important adult stem cell population with a multitude of potential applications in regenerative medicine. The thorough characterization of the bone marrow MSC (BM-MSC) population derived from the BALB/c species was essential, considering the significance of the murine model amongst animal models. In the present study, we examined the effect of gender, age, and in vitro culture on the basic properties (proliferation, differentiation, and immunosuppressive potential) of BM-MSCs. We found a decline in the progenitor frequencies from the BM of adult mice, lower MSC frequencies in all female donors, and an increase in the BM-MSC proliferation rate upon in vitro propagation. We also examined BM-MSCs for the expression of the 3 major embryonic stem cell transcription factors, Oct3/4, Sox-2, and Nanog, as well as 2 mRNA binding proteins, coding region determinant binding protein/insulin-like growth factor 2 mRNA binding protein 1 (
Crd-bp/Imp1
) and Deleted in azoospermia-like (
Dazl
), which are expressed in primitive stem cells, umbilical cord blood-hematopoietic stem cells and amniotic fluid stem cells, respectively. Further, it has been reported that these 2 genes are critical for embryonic development. In this study, therefore, we report, for the first time, the expression of
Crd-bp/Imp1
and
Dazl
in BM-MSCs.
Dazl
,
Oct3/4
, and
Sox2
were detected in relatively low levels in contrast to
Crd-bp/Imp1
, its major target
c-Myc
, as well as
Nanog
, which were expressed redundantly, irrespective of sex, donor age, or in vitro passaging. These findings could further support the extrinsic theory of aging of the MSC population and the potential implication of embryonic genes in adult stem cell physiology. |
| doi_str_mv | 10.1089/scd.2010.0280 |
| format | Article |
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Crd-bp/Imp1
) and Deleted in azoospermia-like (
Dazl
), which are expressed in primitive stem cells, umbilical cord blood-hematopoietic stem cells and amniotic fluid stem cells, respectively. Further, it has been reported that these 2 genes are critical for embryonic development. In this study, therefore, we report, for the first time, the expression of
Crd-bp/Imp1
and
Dazl
in BM-MSCs.
Dazl
,
Oct3/4
, and
Sox2
were detected in relatively low levels in contrast to
Crd-bp/Imp1
, its major target
c-Myc
, as well as
Nanog
, which were expressed redundantly, irrespective of sex, donor age, or in vitro passaging. These findings could further support the extrinsic theory of aging of the MSC population and the potential implication of embryonic genes in adult stem cell physiology.</description><identifier>ISSN: 1547-3287</identifier><identifier>EISSN: 1557-8534</identifier><identifier>DOI: 10.1089/scd.2010.0280</identifier><identifier>PMID: 21204633</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Age Factors ; Animals ; Antigens, Differentiation - metabolism ; Bone Marrow Cells - cytology ; Cell Differentiation ; Cell Proliferation ; Cell Size ; Cells, Cultured ; Cellular Senescence ; Female ; Gene Expression Profiling ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Male ; Mesenchymal Stromal Cells - metabolism ; Mesenchymal Stromal Cells - physiology ; Mice ; Mice, Inbred BALB C ; Nanog Homeobox Protein ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; Original Research Reports ; Phenotype ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism</subject><ispartof>Stem cells and development, 2011-09, Vol.20 (9), p.1549-1561</ispartof><rights>2011, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2011 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-9fadecd37b51637876079e5889ee21c068619b290f55887d06a5dc4f370efa183</citedby><cites>FETCH-LOGICAL-c469t-9fadecd37b51637876079e5889ee21c068619b290f55887d06a5dc4f370efa183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21204633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katsara, Olga</creatorcontrib><creatorcontrib>Mahaira, Louisa G.</creatorcontrib><creatorcontrib>Iliopoulou, Eleni G.</creatorcontrib><creatorcontrib>Moustaki, Ardiana</creatorcontrib><creatorcontrib>Antsaklis, Aristidis</creatorcontrib><creatorcontrib>Loutradis, Dimitrios</creatorcontrib><creatorcontrib>Stefanidis, Konstantinos</creatorcontrib><creatorcontrib>Baxevanis, Constantin N.</creatorcontrib><creatorcontrib>Papamichail, Michael</creatorcontrib><creatorcontrib>Perez, Sonia A.</creatorcontrib><title>Effects of Donor Age, Gender, and In Vitro Cellular Aging on the Phenotypic, Functional, and Molecular Characteristics of Mouse Bone Marrow-Derived Mesenchymal Stem Cells</title><title>Stem cells and development</title><addtitle>Stem Cells Dev</addtitle><description>Mesenchymal stem cells (MSCs) are a very important adult stem cell population with a multitude of potential applications in regenerative medicine. The thorough characterization of the bone marrow MSC (BM-MSC) population derived from the BALB/c species was essential, considering the significance of the murine model amongst animal models. In the present study, we examined the effect of gender, age, and in vitro culture on the basic properties (proliferation, differentiation, and immunosuppressive potential) of BM-MSCs. We found a decline in the progenitor frequencies from the BM of adult mice, lower MSC frequencies in all female donors, and an increase in the BM-MSC proliferation rate upon in vitro propagation. We also examined BM-MSCs for the expression of the 3 major embryonic stem cell transcription factors, Oct3/4, Sox-2, and Nanog, as well as 2 mRNA binding proteins, coding region determinant binding protein/insulin-like growth factor 2 mRNA binding protein 1 (
Crd-bp/Imp1
) and Deleted in azoospermia-like (
Dazl
), which are expressed in primitive stem cells, umbilical cord blood-hematopoietic stem cells and amniotic fluid stem cells, respectively. Further, it has been reported that these 2 genes are critical for embryonic development. In this study, therefore, we report, for the first time, the expression of
Crd-bp/Imp1
and
Dazl
in BM-MSCs.
Dazl
,
Oct3/4
, and
Sox2
were detected in relatively low levels in contrast to
Crd-bp/Imp1
, its major target
c-Myc
, as well as
Nanog
, which were expressed redundantly, irrespective of sex, donor age, or in vitro passaging. These findings could further support the extrinsic theory of aging of the MSC population and the potential implication of embryonic genes in adult stem cell physiology.</description><subject>Age Factors</subject><subject>Animals</subject><subject>Antigens, Differentiation - metabolism</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell Size</subject><subject>Cells, Cultured</subject><subject>Cellular Senescence</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Male</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nanog Homeobox Protein</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Original Research Reports</subject><subject>Phenotype</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>SOXB1 Transcription Factors - metabolism</subject><issn>1547-3287</issn><issn>1557-8534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P1CAYxxujcdfVo1dD4sHLdKS0BXocZ1_cZCea-HIlDDzMYFqYBaqZr-SnlE5XExMTwwF4-P2fF_5F8bLCywrz7m1UeklwvmHC8aPivGpbVvK2bh5P54aVNeHsrHgW4zeMCSW8eVqckYrghtb1efHzyhhQKSJv0KV3PqDVDhboBpyGsEDSaXTr0Febgkdr6PuxlxNi3Q55h9Ie0Mc9OJ-OB6sW6Hp0KlnvZD9LN74HdZKs9zJIlSDYmKw6ldv4MQJ65x2gjQzB_ygv8_N3yDKI4NT-OMgefUownCrH58UTI_sILx72i-LL9dXn9fvy7sPN7Xp1V6qGdqnsjNSgdM22bUVrxhnFrIOW8w6AVApTTqtuSzps2hxkGlPZatWYmmEwsuL1RfFmznsI_n6EmMRgo8odSAe5ZcF521KSM2fy9UzuZA_COuNTHnKixYpQ1rH83SRTy39QeWkYrMrjG5vjfwnKWaCCjzGAEYdgBxmOosJiMl1k08VkuphMz_yrh4bH7QD6D_3b5QzUMzCFpXO9hS2E9J-0vwCHuLgP</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Katsara, Olga</creator><creator>Mahaira, Louisa G.</creator><creator>Iliopoulou, Eleni G.</creator><creator>Moustaki, Ardiana</creator><creator>Antsaklis, Aristidis</creator><creator>Loutradis, Dimitrios</creator><creator>Stefanidis, Konstantinos</creator><creator>Baxevanis, Constantin N.</creator><creator>Papamichail, Michael</creator><creator>Perez, Sonia A.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110901</creationdate><title>Effects of Donor Age, Gender, and In Vitro Cellular Aging on the Phenotypic, Functional, and Molecular Characteristics of Mouse Bone Marrow-Derived Mesenchymal Stem Cells</title><author>Katsara, Olga ; Mahaira, Louisa G. ; Iliopoulou, Eleni G. ; Moustaki, Ardiana ; Antsaklis, Aristidis ; Loutradis, Dimitrios ; Stefanidis, Konstantinos ; Baxevanis, Constantin N. ; Papamichail, Michael ; Perez, Sonia A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-9fadecd37b51637876079e5889ee21c068619b290f55887d06a5dc4f370efa183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Age Factors</topic><topic>Animals</topic><topic>Antigens, Differentiation - metabolism</topic><topic>Bone Marrow Cells - cytology</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cell Size</topic><topic>Cells, Cultured</topic><topic>Cellular Senescence</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Male</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanog Homeobox Protein</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Octamer Transcription Factor-3 - metabolism</topic><topic>Original Research Reports</topic><topic>Phenotype</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>SOXB1 Transcription Factors - genetics</topic><topic>SOXB1 Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katsara, Olga</creatorcontrib><creatorcontrib>Mahaira, Louisa G.</creatorcontrib><creatorcontrib>Iliopoulou, Eleni G.</creatorcontrib><creatorcontrib>Moustaki, Ardiana</creatorcontrib><creatorcontrib>Antsaklis, Aristidis</creatorcontrib><creatorcontrib>Loutradis, Dimitrios</creatorcontrib><creatorcontrib>Stefanidis, Konstantinos</creatorcontrib><creatorcontrib>Baxevanis, Constantin N.</creatorcontrib><creatorcontrib>Papamichail, Michael</creatorcontrib><creatorcontrib>Perez, Sonia A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cells and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katsara, Olga</au><au>Mahaira, Louisa G.</au><au>Iliopoulou, Eleni G.</au><au>Moustaki, Ardiana</au><au>Antsaklis, Aristidis</au><au>Loutradis, Dimitrios</au><au>Stefanidis, Konstantinos</au><au>Baxevanis, Constantin N.</au><au>Papamichail, Michael</au><au>Perez, Sonia A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Donor Age, Gender, and In Vitro Cellular Aging on the Phenotypic, Functional, and Molecular Characteristics of Mouse Bone Marrow-Derived Mesenchymal Stem Cells</atitle><jtitle>Stem cells and development</jtitle><addtitle>Stem Cells Dev</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>20</volume><issue>9</issue><spage>1549</spage><epage>1561</epage><pages>1549-1561</pages><issn>1547-3287</issn><eissn>1557-8534</eissn><abstract>Mesenchymal stem cells (MSCs) are a very important adult stem cell population with a multitude of potential applications in regenerative medicine. The thorough characterization of the bone marrow MSC (BM-MSC) population derived from the BALB/c species was essential, considering the significance of the murine model amongst animal models. In the present study, we examined the effect of gender, age, and in vitro culture on the basic properties (proliferation, differentiation, and immunosuppressive potential) of BM-MSCs. We found a decline in the progenitor frequencies from the BM of adult mice, lower MSC frequencies in all female donors, and an increase in the BM-MSC proliferation rate upon in vitro propagation. We also examined BM-MSCs for the expression of the 3 major embryonic stem cell transcription factors, Oct3/4, Sox-2, and Nanog, as well as 2 mRNA binding proteins, coding region determinant binding protein/insulin-like growth factor 2 mRNA binding protein 1 (
Crd-bp/Imp1
) and Deleted in azoospermia-like (
Dazl
), which are expressed in primitive stem cells, umbilical cord blood-hematopoietic stem cells and amniotic fluid stem cells, respectively. Further, it has been reported that these 2 genes are critical for embryonic development. In this study, therefore, we report, for the first time, the expression of
Crd-bp/Imp1
and
Dazl
in BM-MSCs.
Dazl
,
Oct3/4
, and
Sox2
were detected in relatively low levels in contrast to
Crd-bp/Imp1
, its major target
c-Myc
, as well as
Nanog
, which were expressed redundantly, irrespective of sex, donor age, or in vitro passaging. These findings could further support the extrinsic theory of aging of the MSC population and the potential implication of embryonic genes in adult stem cell physiology.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>21204633</pmid><doi>10.1089/scd.2010.0280</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1547-3287 |
| ispartof | Stem cells and development, 2011-09, Vol.20 (9), p.1549-1561 |
| issn | 1547-3287 1557-8534 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Age Factors Animals Antigens, Differentiation - metabolism Bone Marrow Cells - cytology Cell Differentiation Cell Proliferation Cell Size Cells, Cultured Cellular Senescence Female Gene Expression Profiling Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Male Mesenchymal Stromal Cells - metabolism Mesenchymal Stromal Cells - physiology Mice Mice, Inbred BALB C Nanog Homeobox Protein Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Original Research Reports Phenotype RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism SOXB1 Transcription Factors - genetics SOXB1 Transcription Factors - metabolism |
| title | Effects of Donor Age, Gender, and In Vitro Cellular Aging on the Phenotypic, Functional, and Molecular Characteristics of Mouse Bone Marrow-Derived Mesenchymal Stem Cells |
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