Biophysical Characterization of Complexes of DNA with Mixtures of the Neutral Lipids 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine or 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine and 1,2-Dioleoyl-sn-glycero-3-phosphocholine in the Presence of Bivalent Metal Cations for DNA Transfection
Neutral lipids have received up to now a little attention as genetic material carriers, despite some valuable features, such as the absence of toxicity and the high stability in serum of their complexes with DNA. We have prepared two quaternary complexes of DNA and mixtures of 1, 2-dioleoyl-sn-glyce...
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| Veröffentlicht in: | The journal of physical chemistry. B 2011-09, Vol.115 (34), p.10198-10206 |
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| creator | Pisani, Michela Mobbili, Giovanna Placentino, Immacolata F Smorlesi, Arianna Bruni, Paolo |
| description | Neutral lipids have received up to now a little attention as genetic material carriers, despite some valuable features, such as the absence of toxicity and the high stability in serum of their complexes with DNA. We have prepared two quaternary complexes of DNA and mixtures of 1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine (6PE) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine (12PE) with DOPC in aqueous dispersions of bivalent metal cations (PE/DOPC–DNA–M2+). The aim was to evaluate the effect of the amide moiety on the transfection efficiency. These complexes form in a self-assembled manner, the DNA condensation being promoted by the metal cations. Synchrotron X-ray diffraction analysis was used to determine the structure of the complexes, which exhibit the lamellar symmetry of the Lα c phase. The size and surface charge of the complexes have also been measured, and promising results of DNA transfections in vitro have been reported. |
| doi_str_mv | 10.1021/jp202577u |
| format | Article |
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We have prepared two quaternary complexes of DNA and mixtures of 1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine (6PE) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine (12PE) with DOPC in aqueous dispersions of bivalent metal cations (PE/DOPC–DNA–M2+). The aim was to evaluate the effect of the amide moiety on the transfection efficiency. These complexes form in a self-assembled manner, the DNA condensation being promoted by the metal cations. Synchrotron X-ray diffraction analysis was used to determine the structure of the complexes, which exhibit the lamellar symmetry of the Lα c phase. The size and surface charge of the complexes have also been measured, and promising results of DNA transfections in vitro have been reported.</description><identifier>ISSN: 1520-6106</identifier><identifier>EISSN: 1520-5207</identifier><identifier>DOI: 10.1021/jp202577u</identifier><identifier>PMID: 21790167</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; B: Macromolecules, Soft Matter ; Biophysical Phenomena ; Cations ; Deoxyribonucleic acid ; Diffraction ; Dispersions ; DNA - chemistry ; DNA - genetics ; DNA - metabolism ; Drug Carriers - chemistry ; In vitro testing ; Lamellar structure ; Lipids ; Metals - chemistry ; Mice ; Models, Molecular ; Molecular Conformation ; NIH 3T3 Cells ; Particle Size ; Phosphatidylcholines - chemistry ; Phosphatidylethanolamines - chemistry ; Toxicity ; Transfection - methods ; X-Ray Diffraction</subject><ispartof>The journal of physical chemistry. B, 2011-09, Vol.115 (34), p.10198-10206</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>2011 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a380t-c89a1318b8894a713ab04a417fa98704ce1a2056a4244240a9c5619fd5e872dd3</citedby><cites>FETCH-LOGICAL-a380t-c89a1318b8894a713ab04a417fa98704ce1a2056a4244240a9c5619fd5e872dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jp202577u$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jp202577u$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21790167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pisani, Michela</creatorcontrib><creatorcontrib>Mobbili, Giovanna</creatorcontrib><creatorcontrib>Placentino, Immacolata F</creatorcontrib><creatorcontrib>Smorlesi, Arianna</creatorcontrib><creatorcontrib>Bruni, Paolo</creatorcontrib><title>Biophysical Characterization of Complexes of DNA with Mixtures of the Neutral Lipids 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine or 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine and 1,2-Dioleoyl-sn-glycero-3-phosphocholine in the Presence of Bivalent Metal Cations for DNA Transfection</title><title>The journal of physical chemistry. B</title><addtitle>J. Phys. Chem. B</addtitle><description>Neutral lipids have received up to now a little attention as genetic material carriers, despite some valuable features, such as the absence of toxicity and the high stability in serum of their complexes with DNA. We have prepared two quaternary complexes of DNA and mixtures of 1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine (6PE) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine (12PE) with DOPC in aqueous dispersions of bivalent metal cations (PE/DOPC–DNA–M2+). The aim was to evaluate the effect of the amide moiety on the transfection efficiency. These complexes form in a self-assembled manner, the DNA condensation being promoted by the metal cations. Synchrotron X-ray diffraction analysis was used to determine the structure of the complexes, which exhibit the lamellar symmetry of the Lα c phase. The size and surface charge of the complexes have also been measured, and promising results of DNA transfections in vitro have been reported.</description><subject>Animals</subject><subject>B: Macromolecules, Soft Matter</subject><subject>Biophysical Phenomena</subject><subject>Cations</subject><subject>Deoxyribonucleic acid</subject><subject>Diffraction</subject><subject>Dispersions</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>Drug Carriers - chemistry</subject><subject>In vitro testing</subject><subject>Lamellar structure</subject><subject>Lipids</subject><subject>Metals - chemistry</subject><subject>Mice</subject><subject>Models, Molecular</subject><subject>Molecular Conformation</subject><subject>NIH 3T3 Cells</subject><subject>Particle Size</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Phosphatidylethanolamines - chemistry</subject><subject>Toxicity</subject><subject>Transfection - methods</subject><subject>X-Ray Diffraction</subject><issn>1520-6106</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkk9v1DAQxQMC0VI48AVQLgiQCNhOnDjH7ZZ_0nbhUM7RrDMhXnntYDuw4dPjdJeKA6JC8sie0U9Pfk-TJE8oeU0Jo2-2AyOMV9V4NzmlnJEsVnXv-C4pKU-Sh95vSYSYKB8kJ4xWNaFldXpnca7s0E9eSdDpsgcHMqBTPyEoa1LbpUu7GzTu0c_NxXqR_lChTy_VPozuMAw9pmscg4sKKzWo1qf0FcsulNVoJ515k33Vk0RnszwbeutjYejBWA07ZTBbZz3uYzsd-tS6_xZobYvyDwkw7e0asrd6hpW59vA5-kEjcfZ0rr6DRhPSSwxzMNdx-LSLX5szuHJgfIdynj5K7negPT4-3mfJl3dvr5YfstWn9x-Xi1UGuSAhk6IGmlOxEaIuoKI5bEgBBa06qEVFCokUGOElFKyIh0AteUnrruUoKta2-Vny_KA7OPttRB-anfIStQaDdvSNEJxzQWsSyRf_JGnFc854XrDb0UgJRmvOI_rygEpnvXfYNYNTO3BTQ0kzr2Fzs4aRfXqUHTc7bG_I33sXgWcHAKRvtnZ0Jkb3F6FfWK3olw</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Pisani, Michela</creator><creator>Mobbili, Giovanna</creator><creator>Placentino, Immacolata F</creator><creator>Smorlesi, Arianna</creator><creator>Bruni, Paolo</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20110901</creationdate><title>Biophysical Characterization of Complexes of DNA with Mixtures of the Neutral Lipids 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine or 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine and 1,2-Dioleoyl-sn-glycero-3-phosphocholine in the Presence of Bivalent Metal Cations for DNA Transfection</title><author>Pisani, Michela ; Mobbili, Giovanna ; Placentino, Immacolata F ; Smorlesi, Arianna ; Bruni, Paolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a380t-c89a1318b8894a713ab04a417fa98704ce1a2056a4244240a9c5619fd5e872dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>B: Macromolecules, Soft Matter</topic><topic>Biophysical Phenomena</topic><topic>Cations</topic><topic>Deoxyribonucleic acid</topic><topic>Diffraction</topic><topic>Dispersions</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>Drug Carriers - chemistry</topic><topic>In vitro testing</topic><topic>Lamellar structure</topic><topic>Lipids</topic><topic>Metals - chemistry</topic><topic>Mice</topic><topic>Models, Molecular</topic><topic>Molecular Conformation</topic><topic>NIH 3T3 Cells</topic><topic>Particle Size</topic><topic>Phosphatidylcholines - chemistry</topic><topic>Phosphatidylethanolamines - chemistry</topic><topic>Toxicity</topic><topic>Transfection - methods</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pisani, Michela</creatorcontrib><creatorcontrib>Mobbili, Giovanna</creatorcontrib><creatorcontrib>Placentino, Immacolata F</creatorcontrib><creatorcontrib>Smorlesi, Arianna</creatorcontrib><creatorcontrib>Bruni, Paolo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of physical chemistry. B</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pisani, Michela</au><au>Mobbili, Giovanna</au><au>Placentino, Immacolata F</au><au>Smorlesi, Arianna</au><au>Bruni, Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biophysical Characterization of Complexes of DNA with Mixtures of the Neutral Lipids 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine or 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine and 1,2-Dioleoyl-sn-glycero-3-phosphocholine in the Presence of Bivalent Metal Cations for DNA Transfection</atitle><jtitle>The journal of physical chemistry. B</jtitle><addtitle>J. Phys. Chem. B</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>115</volume><issue>34</issue><spage>10198</spage><epage>10206</epage><pages>10198-10206</pages><issn>1520-6106</issn><eissn>1520-5207</eissn><abstract>Neutral lipids have received up to now a little attention as genetic material carriers, despite some valuable features, such as the absence of toxicity and the high stability in serum of their complexes with DNA. We have prepared two quaternary complexes of DNA and mixtures of 1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine (6PE) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine (12PE) with DOPC in aqueous dispersions of bivalent metal cations (PE/DOPC–DNA–M2+). The aim was to evaluate the effect of the amide moiety on the transfection efficiency. These complexes form in a self-assembled manner, the DNA condensation being promoted by the metal cations. Synchrotron X-ray diffraction analysis was used to determine the structure of the complexes, which exhibit the lamellar symmetry of the Lα c phase. The size and surface charge of the complexes have also been measured, and promising results of DNA transfections in vitro have been reported.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>21790167</pmid><doi>10.1021/jp202577u</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 1520-6106 |
| ispartof | The journal of physical chemistry. B, 2011-09, Vol.115 (34), p.10198-10206 |
| issn | 1520-6106 1520-5207 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Animals B: Macromolecules, Soft Matter Biophysical Phenomena Cations Deoxyribonucleic acid Diffraction Dispersions DNA - chemistry DNA - genetics DNA - metabolism Drug Carriers - chemistry In vitro testing Lamellar structure Lipids Metals - chemistry Mice Models, Molecular Molecular Conformation NIH 3T3 Cells Particle Size Phosphatidylcholines - chemistry Phosphatidylethanolamines - chemistry Toxicity Transfection - methods X-Ray Diffraction |
| title | Biophysical Characterization of Complexes of DNA with Mixtures of the Neutral Lipids 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine or 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine and 1,2-Dioleoyl-sn-glycero-3-phosphocholine in the Presence of Bivalent Metal Cations for DNA Transfection |
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