Development of cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on oxygen electrode for the determination of total cholesterol in food samples

The development of a cholesterol biosensor by co-immobilization of cholesterol esterase (ChEt) and cholesterol oxidase (ChOX) on oxygen electrode is described. The electrode consists of gold cathode and Ag/AgCl anode. The enzymes were immobilized by cross-linking with glutaraldehyde and Bovine Serum...

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Veröffentlicht in:	Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2007-05, Vol.70 (2), p.375-379
Hauptverfasser:	Basu, Anjan Kumar, Chattopadhyay, Parimal, Roychoudhuri, Utpal, Chakraborty, Runu
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques - instrumentation Biosensing Techniques - methods ChEt Cholesterol - analysis Cholesterol biosensor Cholesterol Oxidase - chemistry ChOX Electrochemistry - instrumentation Electrochemistry - methods Electrodes Enzymes, Immobilized - chemistry Equipment Design Equipment Failure Analysis Food Analysis - instrumentation Food Analysis - methods Food sample analysis Food Technology - instrumentation Food Technology - methods Oxygen - chemistry Reproducibility of Results Sensitivity and Specificity Stability Sterol Esterase - chemistry
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