Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells
In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “p...
Gespeichert in:
| Veröffentlicht in: | Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2007-05, Vol.70 (2), p.363-368 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 368 |
|---|---|
| container_issue | 2 |
| container_start_page | 363 |
| container_title | Bioelectrochemistry (Amsterdam, Netherlands) |
| container_volume | 70 |
| creator | He, Huiqi Chang, Donald C. Lee, Yi-Kuen |
| description | In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “phase diagram”, which delineates the boundaries for 1) effective EP of five different size molecules and 2) electric cell lysis at the single-cell level. In addition, these boundary curves (i.e., electric field versus pulse duration) were fitted successfully with an exponential function with three constants. We found that, when the molecular size increases, the corresponding electroporation boundary becomes closer to the electric cell lysis boundary. Based on more than 2000
single-cell measurements on five different size molecules, the critical size of molecule was found to be approximately 40
kDa. Comparing to the traditional instrument, MEMS-based micro electroporation chip can greatly shorten the experimental time. |
| doi_str_mv | 10.1016/j.bioelechem.2006.05.008 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_885050785</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1567539406000818</els_id><sourcerecordid>885050785</sourcerecordid><originalsourceid>FETCH-LOGICAL-c470t-3c7d69743bf9a0ca624c14787a08fe6b6733c0e268f4c862b58836ec190a25db3</originalsourceid><addsrcrecordid>eNqFkE1v3CAQhjm0StKkfyHi1tM6gzEfe2yjNltppVySM8J4nGVrGxdwpah_vji7Uo49geCZd2YeQiiDigGTd8eq9QEHdAccqxpAViAqAP2BXDEh1UbwbXNJPqV0hPLKlLggl0zqGjiHK_L3OfnphVo6ehcDXXNyDHOINvswUXfwM82Bdpgxjn5Cmg9Iw5z9aAc6H16Td-vFRjuuSKJ-ekOWOdtfhexpmW4MJXYZ8O13h3tLHQ5DuiEfezsk_Hw-r8nzj-9P97vN_vHh5_3X_cY1CvKGO9XJrWp4228tOCvrxrFGaWVB9yhbqTh3gLXUfeO0rFuhNZfo2BZsLbqWX5Mvp9w5ht8LpmxGn9YJ7IRhSUZrAQKUFoXUJ7K4SClib-ZYNo2vhoFZbZujebdtVtsGhCleS-ntucnSjti9F55VF-DbCcCy6h-P0STncXLY-Vikmy74_3f5B6-AmZ8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>885050785</pqid></control><display><type>article</type><title>Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>He, Huiqi ; Chang, Donald C. ; Lee, Yi-Kuen</creator><creatorcontrib>He, Huiqi ; Chang, Donald C. ; Lee, Yi-Kuen</creatorcontrib><description>In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “phase diagram”, which delineates the boundaries for 1) effective EP of five different size molecules and 2) electric cell lysis at the single-cell level. In addition, these boundary curves (i.e., electric field versus pulse duration) were fitted successfully with an exponential function with three constants. We found that, when the molecular size increases, the corresponding electroporation boundary becomes closer to the electric cell lysis boundary. Based on more than 2000
single-cell measurements on five different size molecules, the critical size of molecule was found to be approximately 40
kDa. Comparing to the traditional instrument, MEMS-based micro electroporation chip can greatly shorten the experimental time.</description><identifier>ISSN: 1567-5394</identifier><identifier>DOI: 10.1016/j.bioelechem.2006.05.008</identifier><identifier>PMID: 16820330</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3D electrode ; Biopolymers - pharmacokinetics ; Cell Membrane - metabolism ; Cell Membrane - radiation effects ; Cell Membrane Permeability - physiology ; Cell Membrane Permeability - radiation effects ; Computer Simulation ; Dose-Response Relationship, Radiation ; Electroporation - instrumentation ; Electroporation - methods ; HeLa Cells ; Humans ; Membrane permeability ; MEMS ; Micro electroporation ; Models, Biological ; Phase diagram ; Radiation Dosage ; Size effect</subject><ispartof>Bioelectrochemistry (Amsterdam, Netherlands), 2007-05, Vol.70 (2), p.363-368</ispartof><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-3c7d69743bf9a0ca624c14787a08fe6b6733c0e268f4c862b58836ec190a25db3</citedby><cites>FETCH-LOGICAL-c470t-3c7d69743bf9a0ca624c14787a08fe6b6733c0e268f4c862b58836ec190a25db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bioelechem.2006.05.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16820330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Huiqi</creatorcontrib><creatorcontrib>Chang, Donald C.</creatorcontrib><creatorcontrib>Lee, Yi-Kuen</creatorcontrib><title>Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells</title><title>Bioelectrochemistry (Amsterdam, Netherlands)</title><addtitle>Bioelectrochemistry</addtitle><description>In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “phase diagram”, which delineates the boundaries for 1) effective EP of five different size molecules and 2) electric cell lysis at the single-cell level. In addition, these boundary curves (i.e., electric field versus pulse duration) were fitted successfully with an exponential function with three constants. We found that, when the molecular size increases, the corresponding electroporation boundary becomes closer to the electric cell lysis boundary. Based on more than 2000
single-cell measurements on five different size molecules, the critical size of molecule was found to be approximately 40
kDa. Comparing to the traditional instrument, MEMS-based micro electroporation chip can greatly shorten the experimental time.</description><subject>3D electrode</subject><subject>Biopolymers - pharmacokinetics</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - radiation effects</subject><subject>Cell Membrane Permeability - physiology</subject><subject>Cell Membrane Permeability - radiation effects</subject><subject>Computer Simulation</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Electroporation - instrumentation</subject><subject>Electroporation - methods</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Membrane permeability</subject><subject>MEMS</subject><subject>Micro electroporation</subject><subject>Models, Biological</subject><subject>Phase diagram</subject><subject>Radiation Dosage</subject><subject>Size effect</subject><issn>1567-5394</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhjm0StKkfyHi1tM6gzEfe2yjNltppVySM8J4nGVrGxdwpah_vji7Uo49geCZd2YeQiiDigGTd8eq9QEHdAccqxpAViAqAP2BXDEh1UbwbXNJPqV0hPLKlLggl0zqGjiHK_L3OfnphVo6ehcDXXNyDHOINvswUXfwM82Bdpgxjn5Cmg9Iw5z9aAc6H16Td-vFRjuuSKJ-ekOWOdtfhexpmW4MJXYZ8O13h3tLHQ5DuiEfezsk_Hw-r8nzj-9P97vN_vHh5_3X_cY1CvKGO9XJrWp4228tOCvrxrFGaWVB9yhbqTh3gLXUfeO0rFuhNZfo2BZsLbqWX5Mvp9w5ht8LpmxGn9YJ7IRhSUZrAQKUFoXUJ7K4SClib-ZYNo2vhoFZbZujebdtVtsGhCleS-ntucnSjti9F55VF-DbCcCy6h-P0STncXLY-Vikmy74_3f5B6-AmZ8</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>He, Huiqi</creator><creator>Chang, Donald C.</creator><creator>Lee, Yi-Kuen</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20070501</creationdate><title>Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells</title><author>He, Huiqi ; Chang, Donald C. ; Lee, Yi-Kuen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-3c7d69743bf9a0ca624c14787a08fe6b6733c0e268f4c862b58836ec190a25db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>3D electrode</topic><topic>Biopolymers - pharmacokinetics</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - radiation effects</topic><topic>Cell Membrane Permeability - physiology</topic><topic>Cell Membrane Permeability - radiation effects</topic><topic>Computer Simulation</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Electroporation - instrumentation</topic><topic>Electroporation - methods</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Membrane permeability</topic><topic>MEMS</topic><topic>Micro electroporation</topic><topic>Models, Biological</topic><topic>Phase diagram</topic><topic>Radiation Dosage</topic><topic>Size effect</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Huiqi</creatorcontrib><creatorcontrib>Chang, Donald C.</creatorcontrib><creatorcontrib>Lee, Yi-Kuen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Huiqi</au><au>Chang, Donald C.</au><au>Lee, Yi-Kuen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells</atitle><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle><addtitle>Bioelectrochemistry</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>70</volume><issue>2</issue><spage>363</spage><epage>368</epage><pages>363-368</pages><issn>1567-5394</issn><abstract>In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “phase diagram”, which delineates the boundaries for 1) effective EP of five different size molecules and 2) electric cell lysis at the single-cell level. In addition, these boundary curves (i.e., electric field versus pulse duration) were fitted successfully with an exponential function with three constants. We found that, when the molecular size increases, the corresponding electroporation boundary becomes closer to the electric cell lysis boundary. Based on more than 2000
single-cell measurements on five different size molecules, the critical size of molecule was found to be approximately 40
kDa. Comparing to the traditional instrument, MEMS-based micro electroporation chip can greatly shorten the experimental time.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16820330</pmid><doi>10.1016/j.bioelechem.2006.05.008</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1567-5394 |
| ispartof | Bioelectrochemistry (Amsterdam, Netherlands), 2007-05, Vol.70 (2), p.363-368 |
| issn | 1567-5394 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_885050785 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 3D electrode Biopolymers - pharmacokinetics Cell Membrane - metabolism Cell Membrane - radiation effects Cell Membrane Permeability - physiology Cell Membrane Permeability - radiation effects Computer Simulation Dose-Response Relationship, Radiation Electroporation - instrumentation Electroporation - methods HeLa Cells Humans Membrane permeability MEMS Micro electroporation Models, Biological Phase diagram Radiation Dosage Size effect |
| title | Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T05%3A50%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Using%20a%20micro%20electroporation%20chip%20to%20determine%20the%20optimal%20physical%20parameters%20in%20the%20uptake%20of%20biomolecules%20in%20HeLa%20cells&rft.jtitle=Bioelectrochemistry%20(Amsterdam,%20Netherlands)&rft.au=He,%20Huiqi&rft.date=2007-05-01&rft.volume=70&rft.issue=2&rft.spage=363&rft.epage=368&rft.pages=363-368&rft.issn=1567-5394&rft_id=info:doi/10.1016/j.bioelechem.2006.05.008&rft_dat=%3Cproquest_cross%3E885050785%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=885050785&rft_id=info:pmid/16820330&rft_els_id=S1567539406000818&rfr_iscdi=true |