Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue

We describe a label-free imaging method to monitor stem-cell metabolism that discriminates different states of stem cells as they differentiate in living tissues. In this method we use intrinsic fluorescence biomarkers and the phasor approach to fluorescence lifetime imaging microscopy in conjunctio...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2011-08, Vol.108 (33), p.13582-13587
Hauptverfasser:	Stringari, Chiara, Cinquin, Amanda, Cinquin, Olivier, Digman, Michelle A, Donovan, Peter J, Gratton, Enrico
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals biochemical pathways Biological Sciences Biomarkers Caenorhabditis elegans Caenorhabditis elegans - cytology Cell Differentiation Cellular differentiation Cellular metabolism collagen Collagens disease course flavins Fluorescence Fluorescent Dyes Germ cells Germ Cells - cytology Germ Cells - metabolism Half-Life image analysis Imaging Metabolism metabolites Metabolomics - methods Methods Microscopy Microscopy, Fluorescence - methods monitoring NAD (coenzyme) Nematodes Nonnative species Pixels Pluripotent stem cells retinoic acid Stem cells vitamin A
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