AtSAP130/AtSF3b-3 Function is Required for Reproduction in Arabidopsis thaliana
Flowering plants produce multicellular gametophytes through an elaborate regulation of gametogenesis. During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop i...
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| Veröffentlicht in: | Plant and cell physiology 2011-08, Vol.52 (8), p.1330-1339 |
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| container_end_page | 1339 |
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| container_title | Plant and cell physiology |
| container_volume | 52 |
| creator | Aki, Shiori Nakai, Hideto Aoyama, Takashi Oka, Atsuhiro Tsuge, Tomohiko |
| description | Flowering plants produce multicellular gametophytes through an elaborate regulation of gametogenesis. During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop into multicellular gametophytes. Here we report that the Arabidopsis spliceosomal protein, SPLICEOSOME-ASSOCIATED PROTEIN 130 (AtSAP130), is required for proper reproduction. AtSAP130 is encoded by two genes, AtSAP130a and AtSAP130b. Plants with reduced expression of the AtSAP130 genes, induced by RNA interference, showed a defect in fertilization. Besides functional impairment observed in the female reproductive organs, analysis focusing on pollen development revealed defects in the transition from the microspore to the bicellular stage. Our results suggest that AtSAP130a and AtSAP130b play an indispensable role in specific spatiotemporal events in reproduction. |
| doi_str_mv | 10.1093/pcp/pcr077 |
| format | Article |
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During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop into multicellular gametophytes. Here we report that the Arabidopsis spliceosomal protein, SPLICEOSOME-ASSOCIATED PROTEIN 130 (AtSAP130), is required for proper reproduction. AtSAP130 is encoded by two genes, AtSAP130a and AtSAP130b. Plants with reduced expression of the AtSAP130 genes, induced by RNA interference, showed a defect in fertilization. Besides functional impairment observed in the female reproductive organs, analysis focusing on pollen development revealed defects in the transition from the microspore to the bicellular stage. Our results suggest that AtSAP130a and AtSAP130b play an indispensable role in specific spatiotemporal events in reproduction.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcr077</identifier><identifier>PMID: 21680607</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>Arabidopsis - cytology ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Crosses, Genetic ; Gene Duplication - genetics ; Gene Expression Regulation, Plant ; Gene Knockdown Techniques ; Genome, Plant - genetics ; Germination ; Mutation - genetics ; Phenotype ; Phylogeny ; Pollen - cytology ; Pollen - genetics ; Pollen - growth & development ; Reproduction - physiology ; RNA Interference ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Plant and cell physiology, 2011-08, Vol.52 (8), p.1330-1339</ispartof><rights>The Author 2011. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. 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During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop into multicellular gametophytes. Here we report that the Arabidopsis spliceosomal protein, SPLICEOSOME-ASSOCIATED PROTEIN 130 (AtSAP130), is required for proper reproduction. AtSAP130 is encoded by two genes, AtSAP130a and AtSAP130b. Plants with reduced expression of the AtSAP130 genes, induced by RNA interference, showed a defect in fertilization. Besides functional impairment observed in the female reproductive organs, analysis focusing on pollen development revealed defects in the transition from the microspore to the bicellular stage. Our results suggest that AtSAP130a and AtSAP130b play an indispensable role in specific spatiotemporal events in reproduction.</description><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Crosses, Genetic</subject><subject>Gene Duplication - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Knockdown Techniques</subject><subject>Genome, Plant - genetics</subject><subject>Germination</subject><subject>Mutation - genetics</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Pollen - cytology</subject><subject>Pollen - genetics</subject><subject>Pollen - growth & development</subject><subject>Reproduction - physiology</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo7rp68QNILyIIdSedtGmOZXFVWFjxz7mkSYqVbttNmoPf3khXjx6GeTPz4zE8Qi4p3FEQuBzUEMoC50dkThmnsYAUj8kcAJMYeE5n5My5T4CgEU7JLKFZDhnwOdkW42vxTBGWQayxijFa-06NTd9FjYtezN431uio7m0YBttrfzh2UWFl1eh-cAEcP2TbyE6ek5Nats5cHPqCvK_v31aP8Wb78LQqNrFikI9xiiiBmgSF4oyDMFIxpIKh0FqmHMMqZUlW04olCeMZ6oorI7RABpohwwW5mXzDS3tv3FjuGqdM28rO9N6VeY4InKZpIG8nUtneOWvqcrDNTtqvkkL5k18Z8iun_AJ8dbD11c7oP_Q3sABcT0Dvh_-MvgGJiHYH</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Aki, Shiori</creator><creator>Nakai, Hideto</creator><creator>Aoyama, Takashi</creator><creator>Oka, Atsuhiro</creator><creator>Tsuge, Tomohiko</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110801</creationdate><title>AtSAP130/AtSF3b-3 Function is Required for Reproduction in Arabidopsis thaliana</title><author>Aki, Shiori ; Nakai, Hideto ; Aoyama, Takashi ; Oka, Atsuhiro ; Tsuge, Tomohiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-533a01e239c74709eac4319439dda5737095426f1b4224763db7ce9d9340d4343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Arabidopsis - cytology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Crosses, Genetic</topic><topic>Gene Duplication - genetics</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Knockdown Techniques</topic><topic>Genome, Plant - genetics</topic><topic>Germination</topic><topic>Mutation - genetics</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Pollen - cytology</topic><topic>Pollen - genetics</topic><topic>Pollen - growth & development</topic><topic>Reproduction - physiology</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aki, Shiori</creatorcontrib><creatorcontrib>Nakai, Hideto</creatorcontrib><creatorcontrib>Aoyama, Takashi</creatorcontrib><creatorcontrib>Oka, Atsuhiro</creatorcontrib><creatorcontrib>Tsuge, Tomohiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aki, Shiori</au><au>Nakai, Hideto</au><au>Aoyama, Takashi</au><au>Oka, Atsuhiro</au><au>Tsuge, Tomohiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AtSAP130/AtSF3b-3 Function is Required for Reproduction in Arabidopsis thaliana</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>52</volume><issue>8</issue><spage>1330</spage><epage>1339</epage><pages>1330-1339</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Flowering plants produce multicellular gametophytes through an elaborate regulation of gametogenesis. During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop into multicellular gametophytes. Here we report that the Arabidopsis spliceosomal protein, SPLICEOSOME-ASSOCIATED PROTEIN 130 (AtSAP130), is required for proper reproduction. AtSAP130 is encoded by two genes, AtSAP130a and AtSAP130b. Plants with reduced expression of the AtSAP130 genes, induced by RNA interference, showed a defect in fertilization. Besides functional impairment observed in the female reproductive organs, analysis focusing on pollen development revealed defects in the transition from the microspore to the bicellular stage. Our results suggest that AtSAP130a and AtSAP130b play an indispensable role in specific spatiotemporal events in reproduction.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>21680607</pmid><doi>10.1093/pcp/pcr077</doi><tpages>10</tpages></addata></record> |
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| ispartof | Plant and cell physiology, 2011-08, Vol.52 (8), p.1330-1339 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Arabidopsis - cytology Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Carrier Proteins - genetics Carrier Proteins - metabolism Crosses, Genetic Gene Duplication - genetics Gene Expression Regulation, Plant Gene Knockdown Techniques Genome, Plant - genetics Germination Mutation - genetics Phenotype Phylogeny Pollen - cytology Pollen - genetics Pollen - growth & development Reproduction - physiology RNA Interference RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | AtSAP130/AtSF3b-3 Function is Required for Reproduction in Arabidopsis thaliana |
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