Sensitive detection of chemical‐induced genotoxicity by the Cypridina secretory luciferase reporter assay, using DNA repair‐deficient strains of Saccharomyces cerevisiae

Yeast‐based reporter assays are useful for detecting various genotoxic chemicals. We established a genotoxicity assay using recombinant strains of Saccharomyces cerevisiae, each containing a reporter plasmid with the secretory luciferase gene from Cypridina noctiluca, driven by a DNA damage‐responsi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Yeast (Chichester, England) England), 2011-04, Vol.28 (4), p.265-278
Hauptverfasser:	Ochi, Yukari, Sugawara, Harumi, Iwami, Mio, Tanaka, Megumi, Eki, Toshihiko
Format:	Artikel
Sprache:	eng
Schlagworte:	antitumour compounds Cypridina Cypridina secretory luciferase DNA repair DNA Repair - drug effects gene deletion mutants Genes, Reporter genotoxicity Luciferases - genetics Luciferases - metabolism Mutagenicity Tests - instrumentation Mutagenicity Tests - methods Mutagens - toxicity Noctiluca Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism yeast‐based reporter assay
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!