A novel algorithm to generate kymographs from dynamic axons for the quantitative analysis of axonal transport

► Kymographs allow analysis of axonal cargo trajectories from time lapse data. ► We address issues with traditional kymography that prevent analysis of dynamic axons. ► A two-step algorithm incorporated automated image registration and axon detection. ► Axonal contours were detected with a Hough tra...

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Veröffentlicht in:	Journal of neuroscience methods 2011-08, Vol.199 (2), p.230-240
Hauptverfasser:	Chetta, Joshua, Shah, Sameer B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Animals Axonal transport Axonal Transport - physiology Axons - physiology Axons - ultrastructure Cells, Cultured Ganglia, Spinal - cytology Ganglia, Spinal - embryology Ganglia, Spinal - physiology Hough transform Image Cytometry - methods Image registration Kymograph Kymography - methods Neuron Particle tracking Rats Sensory Receptor Cells - cytology Sensory Receptor Cells - physiology Sensory Receptor Cells - ultrastructure
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