Induction of transposase and polyprotein RNA levels in disseminated neoplastic hemocytes of soft-shell clams: Mya arenaria
In Prince Edward Island, a high mortality of soft-shell clams Mya arenaria was found to be related to the disease known as disseminated neoplasia (DN). However, the molecular mechanisms by which hemocytes of clams are transformed in the course of DN remain by far unknown. This study aims at identify...
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| Veröffentlicht in: | Developmental and comparative immunology 2011-02, Vol.35 (2), p.151-154 |
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| creator | Siah, Ahmed McKenna, Patricia Danger, Jean-Michel Johnson, Gerry R. Berthe, Franck C.J. |
| description | In Prince Edward Island, a high mortality of soft-shell clams
Mya arenaria was found to be related to the disease known as disseminated neoplasia (DN). However, the molecular mechanisms by which hemocytes of clams are transformed in the course of DN remain by far unknown. This study aims at identifying the transcripts involved in the development of the disease. Four subtractive cDNA sequence libraries were generated and more than 200,000 reads were obtained. Following similarity searches in genome databases, the transcripts were assigned to cellular functions including mitochondrial respiration, structural proteins, cytoskeleton, nucleic acid regulation, general metabolism, signal transduction, apoptosis, cell cycle regulation, as well as virus transcripts. The expression levels of transposase and polyprotein genes were evaluated in clams with various percentages of tetraploid hemocytes. Data have shown that expression levels were significantly higher in clams with a high percentage of tetraploid hemocytes. These results reinforce the hypothesis of endogenous retrotransposon involvement in the etiology of the disease. Further investigations are needed, however, to elucidate the role of transposase and polyprotein in the disease development. |
| doi_str_mv | 10.1016/j.dci.2010.09.012 |
| format | Article |
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Mya arenaria was found to be related to the disease known as disseminated neoplasia (DN). However, the molecular mechanisms by which hemocytes of clams are transformed in the course of DN remain by far unknown. This study aims at identifying the transcripts involved in the development of the disease. Four subtractive cDNA sequence libraries were generated and more than 200,000 reads were obtained. Following similarity searches in genome databases, the transcripts were assigned to cellular functions including mitochondrial respiration, structural proteins, cytoskeleton, nucleic acid regulation, general metabolism, signal transduction, apoptosis, cell cycle regulation, as well as virus transcripts. The expression levels of transposase and polyprotein genes were evaluated in clams with various percentages of tetraploid hemocytes. Data have shown that expression levels were significantly higher in clams with a high percentage of tetraploid hemocytes. These results reinforce the hypothesis of endogenous retrotransposon involvement in the etiology of the disease. Further investigations are needed, however, to elucidate the role of transposase and polyprotein in the disease development.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2010.09.012</identifier><identifier>PMID: 20932996</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; apoptosis ; Base Sequence ; cell cycle ; Cell Transformation, Neoplastic ; clams ; complementary DNA ; cytoskeleton ; Disseminated neoplasia ; DNA libraries ; DNA, Complementary ; etiology ; Flow Cytometry ; Gene Expression Profiling ; Gene Library ; genes ; Hemocytes ; Hemocytes - metabolism ; Hemocytes - pathology ; metabolism ; mortality ; Mya - genetics ; Mya - metabolism ; Mya - virology ; Mya arenaria ; neoplasms ; Nucleotide Mapping ; Polyploidy ; Polyprotein ; Polyproteins - genetics ; Prince Edward Island ; Retroelements ; retrotransposons ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; signal transduction ; structural proteins ; Tetraploidy ; Transposase ; Transposases - genetics ; viruses</subject><ispartof>Developmental and comparative immunology, 2011-02, Vol.35 (2), p.151-154</ispartof><rights>2010 Elsevier Ltd</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-9948ce8036ba8242e89b7b6fa0010bc62bd47f2b065dc9bd03311f67e2cb41d13</citedby><cites>FETCH-LOGICAL-c408t-9948ce8036ba8242e89b7b6fa0010bc62bd47f2b065dc9bd03311f67e2cb41d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dci.2010.09.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20932996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Siah, Ahmed</creatorcontrib><creatorcontrib>McKenna, Patricia</creatorcontrib><creatorcontrib>Danger, Jean-Michel</creatorcontrib><creatorcontrib>Johnson, Gerry R.</creatorcontrib><creatorcontrib>Berthe, Franck C.J.</creatorcontrib><title>Induction of transposase and polyprotein RNA levels in disseminated neoplastic hemocytes of soft-shell clams: Mya arenaria</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>In Prince Edward Island, a high mortality of soft-shell clams
Mya arenaria was found to be related to the disease known as disseminated neoplasia (DN). However, the molecular mechanisms by which hemocytes of clams are transformed in the course of DN remain by far unknown. This study aims at identifying the transcripts involved in the development of the disease. Four subtractive cDNA sequence libraries were generated and more than 200,000 reads were obtained. Following similarity searches in genome databases, the transcripts were assigned to cellular functions including mitochondrial respiration, structural proteins, cytoskeleton, nucleic acid regulation, general metabolism, signal transduction, apoptosis, cell cycle regulation, as well as virus transcripts. The expression levels of transposase and polyprotein genes were evaluated in clams with various percentages of tetraploid hemocytes. Data have shown that expression levels were significantly higher in clams with a high percentage of tetraploid hemocytes. These results reinforce the hypothesis of endogenous retrotransposon involvement in the etiology of the disease. Further investigations are needed, however, to elucidate the role of transposase and polyprotein in the disease development.</description><subject>Animals</subject><subject>apoptosis</subject><subject>Base Sequence</subject><subject>cell cycle</subject><subject>Cell Transformation, Neoplastic</subject><subject>clams</subject><subject>complementary DNA</subject><subject>cytoskeleton</subject><subject>Disseminated neoplasia</subject><subject>DNA libraries</subject><subject>DNA, Complementary</subject><subject>etiology</subject><subject>Flow Cytometry</subject><subject>Gene Expression Profiling</subject><subject>Gene Library</subject><subject>genes</subject><subject>Hemocytes</subject><subject>Hemocytes - metabolism</subject><subject>Hemocytes - pathology</subject><subject>metabolism</subject><subject>mortality</subject><subject>Mya - genetics</subject><subject>Mya - metabolism</subject><subject>Mya - virology</subject><subject>Mya arenaria</subject><subject>neoplasms</subject><subject>Nucleotide Mapping</subject><subject>Polyploidy</subject><subject>Polyprotein</subject><subject>Polyproteins - genetics</subject><subject>Prince Edward Island</subject><subject>Retroelements</subject><subject>retrotransposons</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>signal transduction</subject><subject>structural proteins</subject><subject>Tetraploidy</subject><subject>Transposase</subject><subject>Transposases - genetics</subject><subject>viruses</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhAbiAb5yyjJ1sYsOpqihUKiABlbhZjj2hXiVx6vFW2j49Xm3hCL5YI33za2Y-xl4KWAsQ7dvt2ruwllBq0GsQ8hFbCdXpCkDpx2wFotlUNWx-nrBnRFsoTwl4yk4k6Fpq3a7Y_eXsdy6HOPM48JzsTEskS8jt7PkSx_2SYsYw829fzviIdzgSL5UPRDiF2Wb0fMa4jJZycPwGp-j2GekQR3HIFd3gOHI32one8c97y23C2aZgn7Mngx0JXzz8p-z64sOP80_V1dePl-dnV5VrQOVK60Y5VFC3vVWykah03_XtYKHs3btW9r7pBtlDu_FO9x7qWoih7VC6vhFe1KfszTG3bHK7Q8pmCuTKULbMvSOjVA2NKBf7Pwmq2ZSzdYUUR9KlSJRwMEsKk017I8Ac3JitKW7MwY0BbYqb0vPqIX3XT-j_dvyRUYDXR2Cw0dhfKZC5_l4S2uJNNnWtCvH-SBQLeBcwGXIBZ4c-JHTZ-Bj-McBveKKptQ</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Siah, Ahmed</creator><creator>McKenna, Patricia</creator><creator>Danger, Jean-Michel</creator><creator>Johnson, Gerry R.</creator><creator>Berthe, Franck C.J.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>7TN</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope></search><sort><creationdate>20110201</creationdate><title>Induction of transposase and polyprotein RNA levels in disseminated neoplastic hemocytes of soft-shell clams: Mya arenaria</title><author>Siah, Ahmed ; McKenna, Patricia ; Danger, Jean-Michel ; Johnson, Gerry R. ; Berthe, Franck C.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-9948ce8036ba8242e89b7b6fa0010bc62bd47f2b065dc9bd03311f67e2cb41d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>apoptosis</topic><topic>Base Sequence</topic><topic>cell cycle</topic><topic>Cell Transformation, Neoplastic</topic><topic>clams</topic><topic>complementary DNA</topic><topic>cytoskeleton</topic><topic>Disseminated neoplasia</topic><topic>DNA libraries</topic><topic>DNA, Complementary</topic><topic>etiology</topic><topic>Flow Cytometry</topic><topic>Gene Expression Profiling</topic><topic>Gene Library</topic><topic>genes</topic><topic>Hemocytes</topic><topic>Hemocytes - metabolism</topic><topic>Hemocytes - pathology</topic><topic>metabolism</topic><topic>mortality</topic><topic>Mya - genetics</topic><topic>Mya - metabolism</topic><topic>Mya - virology</topic><topic>Mya arenaria</topic><topic>neoplasms</topic><topic>Nucleotide Mapping</topic><topic>Polyploidy</topic><topic>Polyprotein</topic><topic>Polyproteins - genetics</topic><topic>Prince Edward Island</topic><topic>Retroelements</topic><topic>retrotransposons</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>signal transduction</topic><topic>structural proteins</topic><topic>Tetraploidy</topic><topic>Transposase</topic><topic>Transposases - genetics</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siah, Ahmed</creatorcontrib><creatorcontrib>McKenna, Patricia</creatorcontrib><creatorcontrib>Danger, Jean-Michel</creatorcontrib><creatorcontrib>Johnson, Gerry R.</creatorcontrib><creatorcontrib>Berthe, Franck C.J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siah, Ahmed</au><au>McKenna, Patricia</au><au>Danger, Jean-Michel</au><au>Johnson, Gerry R.</au><au>Berthe, Franck C.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of transposase and polyprotein RNA levels in disseminated neoplastic hemocytes of soft-shell clams: Mya arenaria</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>35</volume><issue>2</issue><spage>151</spage><epage>154</epage><pages>151-154</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>In Prince Edward Island, a high mortality of soft-shell clams
Mya arenaria was found to be related to the disease known as disseminated neoplasia (DN). However, the molecular mechanisms by which hemocytes of clams are transformed in the course of DN remain by far unknown. This study aims at identifying the transcripts involved in the development of the disease. Four subtractive cDNA sequence libraries were generated and more than 200,000 reads were obtained. Following similarity searches in genome databases, the transcripts were assigned to cellular functions including mitochondrial respiration, structural proteins, cytoskeleton, nucleic acid regulation, general metabolism, signal transduction, apoptosis, cell cycle regulation, as well as virus transcripts. The expression levels of transposase and polyprotein genes were evaluated in clams with various percentages of tetraploid hemocytes. Data have shown that expression levels were significantly higher in clams with a high percentage of tetraploid hemocytes. These results reinforce the hypothesis of endogenous retrotransposon involvement in the etiology of the disease. Further investigations are needed, however, to elucidate the role of transposase and polyprotein in the disease development.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>20932996</pmid><doi>10.1016/j.dci.2010.09.012</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals apoptosis Base Sequence cell cycle Cell Transformation, Neoplastic clams complementary DNA cytoskeleton Disseminated neoplasia DNA libraries DNA, Complementary etiology Flow Cytometry Gene Expression Profiling Gene Library genes Hemocytes Hemocytes - metabolism Hemocytes - pathology metabolism mortality Mya - genetics Mya - metabolism Mya - virology Mya arenaria neoplasms Nucleotide Mapping Polyploidy Polyprotein Polyproteins - genetics Prince Edward Island Retroelements retrotransposons Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Messenger - genetics RNA, Messenger - metabolism signal transduction structural proteins Tetraploidy Transposase Transposases - genetics viruses |
| title | Induction of transposase and polyprotein RNA levels in disseminated neoplastic hemocytes of soft-shell clams: Mya arenaria |
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