Detection of a novel bovine papillomavirus type 11 (BPV-11) using xipapillomavirus consensus polymerase chain reaction primers

Polymerase chain reaction-based bovine papillomavirus (BPV) detection methods using a combination of two primer sets, subAup/subAdw and subBup/subBdw, have enabled the broad-spectrum detection of most characterized BPV types. These methods were used to detect the partial L1 nucleotide sequence of BP...

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Veröffentlicht in:Archives of virology 2011-07, Vol.156 (7), p.1281-1285
Hauptverfasser: Hatama, Shinichi, Ishihara, Ryoko, Ueda, Yasuko, Kanno, Toru, Uchida, Ikuo
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container_issue 7
container_start_page 1281
container_title Archives of virology
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creator Hatama, Shinichi
Ishihara, Ryoko
Ueda, Yasuko
Kanno, Toru
Uchida, Ikuo
description Polymerase chain reaction-based bovine papillomavirus (BPV) detection methods using a combination of two primer sets, subAup/subAdw and subBup/subBdw, have enabled the broad-spectrum detection of most characterized BPV types. These methods were used to detect the partial L1 nucleotide sequence of BPV types from 167 cutaneous warts in cattle. Three potentially new viruses were detected using subBup/subBdw primer sets. The partial nucleotide sequences of these viruses were most similar to BPV-4, -6 and -9. Whole genome sequencing of one sample defines a new BPV type in the genus Xipapillomavirus , designated BPV-11.
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subjects Animals
Biomedical and Life Sciences
Biomedicine
Bovine papillomavirus
Brief Report
Cattle
Cattle Diseases - virology
Cloning
Consensus Sequence
DNA Primers - genetics
Genes
Genomes
Infectious Diseases
Medical Microbiology
Molecular Sequence Data
Phylogenetics
Phylogeny
Polymerase chain reaction
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Skin
Virology
Warts
Warts - veterinary
Warts - virology
Xipapillomavirus - classification
Xipapillomavirus - genetics
Xipapillomavirus - isolation & purification
title Detection of a novel bovine papillomavirus type 11 (BPV-11) using xipapillomavirus consensus polymerase chain reaction primers
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