Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in Southern Brazil
Fusarium head blight is a disease of primary concern to small-grain cereals of Brazil, including barley. Its main causal agent, Fusarium graminearum species complex (Fg complex)¸ is able to produce mycotoxins, especially deoxynivalenol (DON) and nivalenol (NIV), that usually contaminate grain. Strai...
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| Veröffentlicht in: | International journal of food microbiology 2011-08, Vol.148 (3), p.197-201 |
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| creator | Astolfi, P. dos Santos, J. Schneider, L. Gomes, L.B. Silva, C.N. Tessmann, D.J. Del Ponte, E.M. |
| description | Fusarium head blight is a disease of primary concern to small-grain cereals of Brazil, including barley. Its main causal agent,
Fusarium graminearum species complex (Fg complex)¸ is able to produce mycotoxins, especially deoxynivalenol (DON) and nivalenol (NIV), that usually contaminate grain. Strains that produce DON may also produce its acetylated derivatives: 3-acetyl-DON (3-ADON) and 15-acetyl-DON (15-ADON). Ninety two isolates were obtained from samplings of barley grain during three years (2007, 2008 and 2009) from several fields in both southern and northern production regions of Rio Grande do Sul state, Brazil. These isolates were examined for polymerase chain-reaction-based (PCR) trichothecene genotype based on the amplification of portions of
Tri3 and
Tri12. There was no effect of year or region on the proportion of trichothecene genotypes. Overall, 66% of the strains (61/92) were 15-ADON, 4.4% (4/92) were 3-ADON and 29.3% (27/92) were NIV. The overall NIV/DON ratio estimated (0.41) was five times higher than that found in previous studies with strains from wheat grown in the same region. Species identification of nine strains representing the trichothecene genotypes, based on comparisons of DNA sequences of portions of the PHO, RED and URA genes with sequences from curated reference isolates of
Fusarium from GenBank, revealed that they belong to
F. graminearum sensu stricto (four 15-ADON and one 3-ADON strain),
F. meridionale (three NIV strains) and
F. austroamericanum (one 3-ADON strain). These results add to the current regional knowledge of trichothecene genotypes and species within the Fg complex affecting barley in the region.
► PCR assays were used to chemotype
F. graminearum isolates from barley in Brazil. ► Most isolates (61 out of 92 total) belonged to 15-acetyl-deoxynivalenol genotype. ► 27 isolates presented a nivalenol and 4 isolates a 3-acetyl-deoxynivalenol genotype. ► All nivalenol genotypes belonged to
F. meridionale. ► All 15-acetyl-deoxynivalenol belonged to
F. graminearum sensu stricto and the only 3-acetyl-deoxynivalenol to
F. austroamericanum. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2011.05.019 |
| format | Article |
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Fusarium graminearum species complex (Fg complex)¸ is able to produce mycotoxins, especially deoxynivalenol (DON) and nivalenol (NIV), that usually contaminate grain. Strains that produce DON may also produce its acetylated derivatives: 3-acetyl-DON (3-ADON) and 15-acetyl-DON (15-ADON). Ninety two isolates were obtained from samplings of barley grain during three years (2007, 2008 and 2009) from several fields in both southern and northern production regions of Rio Grande do Sul state, Brazil. These isolates were examined for polymerase chain-reaction-based (PCR) trichothecene genotype based on the amplification of portions of
Tri3 and
Tri12. There was no effect of year or region on the proportion of trichothecene genotypes. Overall, 66% of the strains (61/92) were 15-ADON, 4.4% (4/92) were 3-ADON and 29.3% (27/92) were NIV. The overall NIV/DON ratio estimated (0.41) was five times higher than that found in previous studies with strains from wheat grown in the same region. Species identification of nine strains representing the trichothecene genotypes, based on comparisons of DNA sequences of portions of the PHO, RED and URA genes with sequences from curated reference isolates of
Fusarium from GenBank, revealed that they belong to
F. graminearum sensu stricto (four 15-ADON and one 3-ADON strain),
F. meridionale (three NIV strains) and
F. austroamericanum (one 3-ADON strain). These results add to the current regional knowledge of trichothecene genotypes and species within the Fg complex affecting barley in the region.
► PCR assays were used to chemotype
F. graminearum isolates from barley in Brazil. ► Most isolates (61 out of 92 total) belonged to 15-acetyl-deoxynivalenol genotype. ► 27 isolates presented a nivalenol and 4 isolates a 3-acetyl-deoxynivalenol genotype. ► All nivalenol genotypes belonged to
F. meridionale. ► All 15-acetyl-deoxynivalenol belonged to
F. graminearum sensu stricto and the only 3-acetyl-deoxynivalenol to
F. austroamericanum.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2011.05.019</identifier><identifier>PMID: 21665312</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacterial Typing Techniques ; Blight ; Brazil ; Cereals ; Deoxynivalenol ; DNA, Fungal - genetics ; Fusarium - classification ; Fusarium - genetics ; Fusarium - isolation & purification ; Fusarium graminearum ; Fusarium head blight ; Genotype ; Genotypes ; Grain ; Head ; Hordeum - microbiology ; Hordeum vulgare ; Mycotoxins ; Mycotoxins - analysis ; Mycotoxins - genetics ; Nivalenol ; Nucleotide sequence ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Sampling ; trichothecenes ; Trichothecenes - analysis ; Trichothecenes - genetics ; Triticum aestivum ; Vomitoxin</subject><ispartof>International journal of food microbiology, 2011-08, Vol.148 (3), p.197-201</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-940389af4088b74813425baf9ddf4981f318b4422776c83fe0e50a259e157e623</citedby><cites>FETCH-LOGICAL-c408t-940389af4088b74813425baf9ddf4981f318b4422776c83fe0e50a259e157e623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168160511003023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21665312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Astolfi, P.</creatorcontrib><creatorcontrib>dos Santos, J.</creatorcontrib><creatorcontrib>Schneider, L.</creatorcontrib><creatorcontrib>Gomes, L.B.</creatorcontrib><creatorcontrib>Silva, C.N.</creatorcontrib><creatorcontrib>Tessmann, D.J.</creatorcontrib><creatorcontrib>Del Ponte, E.M.</creatorcontrib><title>Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in Southern Brazil</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Fusarium head blight is a disease of primary concern to small-grain cereals of Brazil, including barley. Its main causal agent,
Fusarium graminearum species complex (Fg complex)¸ is able to produce mycotoxins, especially deoxynivalenol (DON) and nivalenol (NIV), that usually contaminate grain. Strains that produce DON may also produce its acetylated derivatives: 3-acetyl-DON (3-ADON) and 15-acetyl-DON (15-ADON). Ninety two isolates were obtained from samplings of barley grain during three years (2007, 2008 and 2009) from several fields in both southern and northern production regions of Rio Grande do Sul state, Brazil. These isolates were examined for polymerase chain-reaction-based (PCR) trichothecene genotype based on the amplification of portions of
Tri3 and
Tri12. There was no effect of year or region on the proportion of trichothecene genotypes. Overall, 66% of the strains (61/92) were 15-ADON, 4.4% (4/92) were 3-ADON and 29.3% (27/92) were NIV. The overall NIV/DON ratio estimated (0.41) was five times higher than that found in previous studies with strains from wheat grown in the same region. Species identification of nine strains representing the trichothecene genotypes, based on comparisons of DNA sequences of portions of the PHO, RED and URA genes with sequences from curated reference isolates of
Fusarium from GenBank, revealed that they belong to
F. graminearum sensu stricto (four 15-ADON and one 3-ADON strain),
F. meridionale (three NIV strains) and
F. austroamericanum (one 3-ADON strain). These results add to the current regional knowledge of trichothecene genotypes and species within the Fg complex affecting barley in the region.
► PCR assays were used to chemotype
F. graminearum isolates from barley in Brazil. ► Most isolates (61 out of 92 total) belonged to 15-acetyl-deoxynivalenol genotype. ► 27 isolates presented a nivalenol and 4 isolates a 3-acetyl-deoxynivalenol genotype. ► All nivalenol genotypes belonged to
F. meridionale. ► All 15-acetyl-deoxynivalenol belonged to
F. graminearum sensu stricto and the only 3-acetyl-deoxynivalenol to
F. austroamericanum.</description><subject>Bacterial Typing Techniques</subject><subject>Blight</subject><subject>Brazil</subject><subject>Cereals</subject><subject>Deoxynivalenol</subject><subject>DNA, Fungal - genetics</subject><subject>Fusarium - classification</subject><subject>Fusarium - genetics</subject><subject>Fusarium - isolation & purification</subject><subject>Fusarium graminearum</subject><subject>Fusarium head blight</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Grain</subject><subject>Head</subject><subject>Hordeum - microbiology</subject><subject>Hordeum vulgare</subject><subject>Mycotoxins</subject><subject>Mycotoxins - analysis</subject><subject>Mycotoxins - genetics</subject><subject>Nivalenol</subject><subject>Nucleotide sequence</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sampling</subject><subject>trichothecenes</subject><subject>Trichothecenes - analysis</subject><subject>Trichothecenes - genetics</subject><subject>Triticum aestivum</subject><subject>Vomitoxin</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EokvhLyBz4pQwjmPHPsKqBaQiDsDZcpxx61USBzupWH49Xm1B3OA0M5pv3kjvEfKKQc2AyTeHOhx8jMMUXIp1A4zVIGpg-hHZMdXpircSHpNdYVXFJIgL8iznAwAIzuEpuWiYlIKzZkfSpzii20abaN7SPR5p9HRNwd3F9Q4dzkhvcY7rccF8Wl1v2aawTfQ22SnMaFPp84IulL2L0zLiD-pTnGhv01jkwky_xK1opZm-S_ZnGJ-TJ96OGV881Evy7frq6_5DdfP5_cf925vKtaDWSrfAlba-DKrvWsV424jeej0MvtWKec5U37ZN03XSKe4RUIBthEYmOpQNvySvz7pLit83zKuZQnY4jnbGuGWjFAcutOz-TXYCZMv1idRnshifc0JvlhQmm46GgTllYw7mr2zMKRsDwpRsyu3Lhy9bP-Hw5_J3GAXYnwEsrtwHTCYXW2eHQ0joVjPE8B9vfgG1VKeB</recordid><startdate>20110815</startdate><enddate>20110815</enddate><creator>Astolfi, P.</creator><creator>dos Santos, J.</creator><creator>Schneider, L.</creator><creator>Gomes, L.B.</creator><creator>Silva, C.N.</creator><creator>Tessmann, D.J.</creator><creator>Del Ponte, E.M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20110815</creationdate><title>Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in Southern Brazil</title><author>Astolfi, P. ; dos Santos, J. ; Schneider, L. ; Gomes, L.B. ; Silva, C.N. ; Tessmann, D.J. ; Del Ponte, E.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-940389af4088b74813425baf9ddf4981f318b4422776c83fe0e50a259e157e623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacterial Typing Techniques</topic><topic>Blight</topic><topic>Brazil</topic><topic>Cereals</topic><topic>Deoxynivalenol</topic><topic>DNA, Fungal - genetics</topic><topic>Fusarium - classification</topic><topic>Fusarium - genetics</topic><topic>Fusarium - isolation & purification</topic><topic>Fusarium graminearum</topic><topic>Fusarium head blight</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Grain</topic><topic>Head</topic><topic>Hordeum - microbiology</topic><topic>Hordeum vulgare</topic><topic>Mycotoxins</topic><topic>Mycotoxins - analysis</topic><topic>Mycotoxins - genetics</topic><topic>Nivalenol</topic><topic>Nucleotide sequence</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sampling</topic><topic>trichothecenes</topic><topic>Trichothecenes - analysis</topic><topic>Trichothecenes - genetics</topic><topic>Triticum aestivum</topic><topic>Vomitoxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Astolfi, P.</creatorcontrib><creatorcontrib>dos Santos, J.</creatorcontrib><creatorcontrib>Schneider, L.</creatorcontrib><creatorcontrib>Gomes, L.B.</creatorcontrib><creatorcontrib>Silva, C.N.</creatorcontrib><creatorcontrib>Tessmann, D.J.</creatorcontrib><creatorcontrib>Del Ponte, E.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Astolfi, P.</au><au>dos Santos, J.</au><au>Schneider, L.</au><au>Gomes, L.B.</au><au>Silva, C.N.</au><au>Tessmann, D.J.</au><au>Del Ponte, E.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in Southern Brazil</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2011-08-15</date><risdate>2011</risdate><volume>148</volume><issue>3</issue><spage>197</spage><epage>201</epage><pages>197-201</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><abstract>Fusarium head blight is a disease of primary concern to small-grain cereals of Brazil, including barley. Its main causal agent,
Fusarium graminearum species complex (Fg complex)¸ is able to produce mycotoxins, especially deoxynivalenol (DON) and nivalenol (NIV), that usually contaminate grain. Strains that produce DON may also produce its acetylated derivatives: 3-acetyl-DON (3-ADON) and 15-acetyl-DON (15-ADON). Ninety two isolates were obtained from samplings of barley grain during three years (2007, 2008 and 2009) from several fields in both southern and northern production regions of Rio Grande do Sul state, Brazil. These isolates were examined for polymerase chain-reaction-based (PCR) trichothecene genotype based on the amplification of portions of
Tri3 and
Tri12. There was no effect of year or region on the proportion of trichothecene genotypes. Overall, 66% of the strains (61/92) were 15-ADON, 4.4% (4/92) were 3-ADON and 29.3% (27/92) were NIV. The overall NIV/DON ratio estimated (0.41) was five times higher than that found in previous studies with strains from wheat grown in the same region. Species identification of nine strains representing the trichothecene genotypes, based on comparisons of DNA sequences of portions of the PHO, RED and URA genes with sequences from curated reference isolates of
Fusarium from GenBank, revealed that they belong to
F. graminearum sensu stricto (four 15-ADON and one 3-ADON strain),
F. meridionale (three NIV strains) and
F. austroamericanum (one 3-ADON strain). These results add to the current regional knowledge of trichothecene genotypes and species within the Fg complex affecting barley in the region.
► PCR assays were used to chemotype
F. graminearum isolates from barley in Brazil. ► Most isolates (61 out of 92 total) belonged to 15-acetyl-deoxynivalenol genotype. ► 27 isolates presented a nivalenol and 4 isolates a 3-acetyl-deoxynivalenol genotype. ► All nivalenol genotypes belonged to
F. meridionale. ► All 15-acetyl-deoxynivalenol belonged to
F. graminearum sensu stricto and the only 3-acetyl-deoxynivalenol to
F. austroamericanum.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21665312</pmid><doi>10.1016/j.ijfoodmicro.2011.05.019</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial Typing Techniques Blight Brazil Cereals Deoxynivalenol DNA, Fungal - genetics Fusarium - classification Fusarium - genetics Fusarium - isolation & purification Fusarium graminearum Fusarium head blight Genotype Genotypes Grain Head Hordeum - microbiology Hordeum vulgare Mycotoxins Mycotoxins - analysis Mycotoxins - genetics Nivalenol Nucleotide sequence Polymerase chain reaction Polymerase Chain Reaction - methods Sampling trichothecenes Trichothecenes - analysis Trichothecenes - genetics Triticum aestivum Vomitoxin |
| title | Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in Southern Brazil |
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