Effect of arsenic in endochondral ossification of experimental animals
Arsenic (As) toxicity is a global health problem affecting millions of people, the most toxic forms being Arsenites [As(III)] and Arsenates [As(V)]. Arsenic intoxication can occur through different exposure routes. The aim of the present work was to determine the effect of As on endochondral ossific...
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| Veröffentlicht in: | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2010-05, Vol.62 (3), p.243-249 |
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| container_title | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie |
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| creator | Aybar Odstrcil, Ana del Carmen Carino, Silvia Norma Diaz Ricci, Juan Carlos Mandalunis, Patricia Mónica |
| description | Arsenic (As) toxicity is a global health problem affecting millions of people, the most toxic forms being Arsenites [As(III)] and Arsenates [As(V)]. Arsenic intoxication can occur through different exposure routes. The aim of the present work was to determine the effect of As on endochondral ossification and bone remodeling in experimental animals, by means of biochemical, histologic, and histomorphometric determinations.
Sixteen male Wistar rats, 100g body weight (b.w.), were divided into two groups: experimental group (n=8), treated with 10mg/l of NaAsO2 in their drinking water, receiving 0.21mg/kgb.w./day during 45 days; and control group (n=8) remained untreated. On day 45, blood samples were obtained by cardiac puncture to perform hematologic blood counts and biochemical determination. The animals were killed, the tibiae, femurs, kidneys and livers were resected, fixed in formalin and processed histologically. Tibia and femur sections were obtained and stained with H&E. The following histomorphometric parameters were determined on tibia and femur sections: bone volume (BV/TV), thickness of growth plate cartilage (GPC.Th) and thickness of hypertrophic zone (HpZ.Th).
Biochemical determinations showed that experimental animals exhibited neutrophilia and a decrease in lymphocytes and monocytes. As levels were below 1μg/dl in both groups. The femur sections of the experimental group showed (1) a statistically significant increase in total growth cartilage plate thickness (p |
| doi_str_mv | 10.1016/j.etp.2009.04.001 |
| format | Article |
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Sixteen male Wistar rats, 100g body weight (b.w.), were divided into two groups: experimental group (n=8), treated with 10mg/l of NaAsO2 in their drinking water, receiving 0.21mg/kgb.w./day during 45 days; and control group (n=8) remained untreated. On day 45, blood samples were obtained by cardiac puncture to perform hematologic blood counts and biochemical determination. The animals were killed, the tibiae, femurs, kidneys and livers were resected, fixed in formalin and processed histologically. Tibia and femur sections were obtained and stained with H&E. The following histomorphometric parameters were determined on tibia and femur sections: bone volume (BV/TV), thickness of growth plate cartilage (GPC.Th) and thickness of hypertrophic zone (HpZ.Th).
Biochemical determinations showed that experimental animals exhibited neutrophilia and a decrease in lymphocytes and monocytes. As levels were below 1μg/dl in both groups. The femur sections of the experimental group showed (1) a statistically significant increase in total growth cartilage plate thickness (p<0.05) at the expense of the hypertrophic zone (p<0.05); (2) subchondral trabecular bone sealed to the growth plate with a non-significant increase in primary spongiosa bone volume. These results suggest that As alters endochondral ossification.</description><identifier>ISSN: 0940-2993</identifier><identifier>EISSN: 1618-1433</identifier><identifier>DOI: 10.1016/j.etp.2009.04.001</identifier><identifier>PMID: 19447590</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Animals ; Arsenic ; Arsenic - toxicity ; Biological and medical sciences ; Bone (endochondral) ; Bone (trabecular) ; Bone and Bones - drug effects ; Bone growth ; Bone histomorphometry ; Bone remodelling ; Cartilage ; Chromatography, High Pressure Liquid ; Drinking water ; Endochondral ossification ; Femur ; Growth plate ; Growth Plate - drug effects ; Heart ; Intoxication ; Investigative techniques, diagnostic techniques (general aspects) ; Kidney ; Kidney - drug effects ; Kidney - pathology ; Liver ; Liver - drug effects ; Liver - pathology ; Male ; Medical sciences ; Monocytes ; Neutrophilia ; Ossification ; Osteogenesis - drug effects ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Rats ; Rats, Wistar ; Statistical analysis ; Tibia ; Toxicity</subject><ispartof>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2010-05, Vol.62 (3), p.243-249</ispartof><rights>2009</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-36d723e3dff686291c1a56fe144cffcac7b531ea207af806b0185bfa7a11b5d73</citedby><cites>FETCH-LOGICAL-c457t-36d723e3dff686291c1a56fe144cffcac7b531ea207af806b0185bfa7a11b5d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.etp.2009.04.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22848097$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19447590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aybar Odstrcil, Ana del Carmen</creatorcontrib><creatorcontrib>Carino, Silvia Norma</creatorcontrib><creatorcontrib>Diaz Ricci, Juan Carlos</creatorcontrib><creatorcontrib>Mandalunis, Patricia Mónica</creatorcontrib><title>Effect of arsenic in endochondral ossification of experimental animals</title><title>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</title><addtitle>Exp Toxicol Pathol</addtitle><description>Arsenic (As) toxicity is a global health problem affecting millions of people, the most toxic forms being Arsenites [As(III)] and Arsenates [As(V)]. Arsenic intoxication can occur through different exposure routes. The aim of the present work was to determine the effect of As on endochondral ossification and bone remodeling in experimental animals, by means of biochemical, histologic, and histomorphometric determinations.
Sixteen male Wistar rats, 100g body weight (b.w.), were divided into two groups: experimental group (n=8), treated with 10mg/l of NaAsO2 in their drinking water, receiving 0.21mg/kgb.w./day during 45 days; and control group (n=8) remained untreated. On day 45, blood samples were obtained by cardiac puncture to perform hematologic blood counts and biochemical determination. The animals were killed, the tibiae, femurs, kidneys and livers were resected, fixed in formalin and processed histologically. Tibia and femur sections were obtained and stained with H&E. The following histomorphometric parameters were determined on tibia and femur sections: bone volume (BV/TV), thickness of growth plate cartilage (GPC.Th) and thickness of hypertrophic zone (HpZ.Th).
Biochemical determinations showed that experimental animals exhibited neutrophilia and a decrease in lymphocytes and monocytes. As levels were below 1μg/dl in both groups. The femur sections of the experimental group showed (1) a statistically significant increase in total growth cartilage plate thickness (p<0.05) at the expense of the hypertrophic zone (p<0.05); (2) subchondral trabecular bone sealed to the growth plate with a non-significant increase in primary spongiosa bone volume. These results suggest that As alters endochondral ossification.</description><subject>Animals</subject><subject>Arsenic</subject><subject>Arsenic - toxicity</subject><subject>Biological and medical sciences</subject><subject>Bone (endochondral)</subject><subject>Bone (trabecular)</subject><subject>Bone and Bones - drug effects</subject><subject>Bone growth</subject><subject>Bone histomorphometry</subject><subject>Bone remodelling</subject><subject>Cartilage</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Drinking water</subject><subject>Endochondral ossification</subject><subject>Femur</subject><subject>Growth plate</subject><subject>Growth Plate - drug effects</subject><subject>Heart</subject><subject>Intoxication</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kidney</subject><subject>Kidney - drug effects</subject><subject>Kidney - pathology</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Monocytes</subject><subject>Neutrophilia</subject><subject>Ossification</subject><subject>Osteogenesis - drug effects</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Statistical analysis</subject><subject>Tibia</subject><subject>Toxicity</subject><issn>0940-2993</issn><issn>1618-1433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LJDEQhoOs6PjxA7xIXxZP3VYl6Y_gaRG_QPCi55BOVzBDT3pMemT992aYYb3tqQ71vMVbD2MXCBUCNtfLiuZ1xQFUBbICwAO2wAa7EqUQv9gClISSKyWO2UlKSwAOqsYjdoxKyrZWsGD3d86RnYvJFSYmCt4WPhQUhsm-T2GIZiymlLzz1sx-CluO_q4p-hWFOS9N8CszpjN26PKg8_08ZW_3d6-3j-Xzy8PT7Z_n0sq6nUvRDC0XJAbnmq7hCi2aunGEUlrnrLFtXwskw6E1roOmB-zq3pnWIPb10IpTdrW7u47Tx4bSrFc-WRpHE2jaJN11AgRva8wk7kgb8wORnF7n0iZ-aQS9taeXOtvTW3sapM72cuZyf33Tr2j4Sex1ZeD3HjDJmtFFE6xP_zjOO9mB2ta82XGUXXx6ijpZT8HS4GO2rYfJ_6fGN0CdjTA</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Aybar Odstrcil, Ana del Carmen</creator><creator>Carino, Silvia Norma</creator><creator>Diaz Ricci, Juan Carlos</creator><creator>Mandalunis, Patricia Mónica</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20100501</creationdate><title>Effect of arsenic in endochondral ossification of experimental animals</title><author>Aybar Odstrcil, Ana del Carmen ; Carino, Silvia Norma ; Diaz Ricci, Juan Carlos ; Mandalunis, Patricia Mónica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-36d723e3dff686291c1a56fe144cffcac7b531ea207af806b0185bfa7a11b5d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Arsenic</topic><topic>Arsenic - toxicity</topic><topic>Biological and medical sciences</topic><topic>Bone (endochondral)</topic><topic>Bone (trabecular)</topic><topic>Bone and Bones - drug effects</topic><topic>Bone growth</topic><topic>Bone histomorphometry</topic><topic>Bone remodelling</topic><topic>Cartilage</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Drinking water</topic><topic>Endochondral ossification</topic><topic>Femur</topic><topic>Growth plate</topic><topic>Growth Plate - drug effects</topic><topic>Heart</topic><topic>Intoxication</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kidney</topic><topic>Kidney - drug effects</topic><topic>Kidney - pathology</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Monocytes</topic><topic>Neutrophilia</topic><topic>Ossification</topic><topic>Osteogenesis - drug effects</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Statistical analysis</topic><topic>Tibia</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aybar Odstrcil, Ana del Carmen</creatorcontrib><creatorcontrib>Carino, Silvia Norma</creatorcontrib><creatorcontrib>Diaz Ricci, Juan Carlos</creatorcontrib><creatorcontrib>Mandalunis, Patricia Mónica</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aybar Odstrcil, Ana del Carmen</au><au>Carino, Silvia Norma</au><au>Diaz Ricci, Juan Carlos</au><au>Mandalunis, Patricia Mónica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of arsenic in endochondral ossification of experimental animals</atitle><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle><addtitle>Exp Toxicol Pathol</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>62</volume><issue>3</issue><spage>243</spage><epage>249</epage><pages>243-249</pages><issn>0940-2993</issn><eissn>1618-1433</eissn><abstract>Arsenic (As) toxicity is a global health problem affecting millions of people, the most toxic forms being Arsenites [As(III)] and Arsenates [As(V)]. Arsenic intoxication can occur through different exposure routes. The aim of the present work was to determine the effect of As on endochondral ossification and bone remodeling in experimental animals, by means of biochemical, histologic, and histomorphometric determinations.
Sixteen male Wistar rats, 100g body weight (b.w.), were divided into two groups: experimental group (n=8), treated with 10mg/l of NaAsO2 in their drinking water, receiving 0.21mg/kgb.w./day during 45 days; and control group (n=8) remained untreated. On day 45, blood samples were obtained by cardiac puncture to perform hematologic blood counts and biochemical determination. The animals were killed, the tibiae, femurs, kidneys and livers were resected, fixed in formalin and processed histologically. Tibia and femur sections were obtained and stained with H&E. The following histomorphometric parameters were determined on tibia and femur sections: bone volume (BV/TV), thickness of growth plate cartilage (GPC.Th) and thickness of hypertrophic zone (HpZ.Th).
Biochemical determinations showed that experimental animals exhibited neutrophilia and a decrease in lymphocytes and monocytes. As levels were below 1μg/dl in both groups. The femur sections of the experimental group showed (1) a statistically significant increase in total growth cartilage plate thickness (p<0.05) at the expense of the hypertrophic zone (p<0.05); (2) subchondral trabecular bone sealed to the growth plate with a non-significant increase in primary spongiosa bone volume. These results suggest that As alters endochondral ossification.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>19447590</pmid><doi>10.1016/j.etp.2009.04.001</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Arsenic Arsenic - toxicity Biological and medical sciences Bone (endochondral) Bone (trabecular) Bone and Bones - drug effects Bone growth Bone histomorphometry Bone remodelling Cartilage Chromatography, High Pressure Liquid Drinking water Endochondral ossification Femur Growth plate Growth Plate - drug effects Heart Intoxication Investigative techniques, diagnostic techniques (general aspects) Kidney Kidney - drug effects Kidney - pathology Liver Liver - drug effects Liver - pathology Male Medical sciences Monocytes Neutrophilia Ossification Osteogenesis - drug effects Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Rats Rats, Wistar Statistical analysis Tibia Toxicity |
| title | Effect of arsenic in endochondral ossification of experimental animals |
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