Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro
Abstract Morphology, immunocytochemistry, growth curve assay, and flow cytometry were used to investigate the effects of all-trans retinoic acid (RA) on cell proliferation, cell cycle progression and differentiation of the astrocytoma cell line SHG-44 from glioblastoma multiforme (World Health Organ...
Gespeichert in:
| Veröffentlicht in: | Journal of clinical neuroscience 2009-02, Vol.16 (2), p.285-294 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 294 |
|---|---|
| container_issue | 2 |
| container_start_page | 285 |
| container_title | Journal of clinical neuroscience |
| container_volume | 16 |
| creator | Zeng, Yi Yang, Zhong Xu, Jian-Guo Yang, Meng-Su Zeng, Zhi-Xiong You, Chao |
| description | Abstract Morphology, immunocytochemistry, growth curve assay, and flow cytometry were used to investigate the effects of all-trans retinoic acid (RA) on cell proliferation, cell cycle progression and differentiation of the astrocytoma cell line SHG-44 from glioblastoma multiforme (World Health Organization grade IV). The differentially expressed genes from RA-treated and normal SHG-44 were identified by cDNA microarray after the cell line SHG-44 was treated with 10 μM RA for 3 days. Validation of some differentially expressed genes was performed by Northern Blot analysis. The expression of glial fibrillary acidic protein (GFAP) was markedly increased in RA-treated SHG-44 cells. Other changes included a short shuttle shape, small nucleus, decreased karyoplasm proportion, the formation of increased thin cytoplasmic processes, reduced cell growth and a 15% increase in G0/G1 phase cell populations. In addition, 42 known genes were identified with altered expression in our cDNA microarray. There was stable down-regulation of MDM2 and UGB as well as overexpression of SOD2, CSTB, and G3BP when RA-treated SHG-44 was compared with normal SHG-44. RA simultaneously suppressed the proliferation of SHG-44 cells significantly as well as induced differentiation and altered gene expression. |
| doi_str_mv | 10.1016/j.jocn.2007.11.014 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_883031288</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0967586808001057</els_id><sourcerecordid>66809118</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-ba4204e9f02a4e176d7f210b7a833adb2cd2a0022dd69975745918a7b358b7d23</originalsourceid><addsrcrecordid>eNp9kkFv1DAUhC0EotuFP8AB-QSnhGfHiRMJIVWFtkiVOBTOlmO_tA5JvNjewv57HO1KSBx6epeZ0dN8Q8gbBiUD1nwYy9GbpeQAsmSsBCaekQ2rK17wpq6ekw10jSzqtmnPyHmMIwB0ooKX5Ix10LFawobEz24YMOCSnJ6mA8U_u4AxoqX3uGCkQ_AzTQ9I7yfn-0nH5GdNDU4TndyC9O7muhCCpoA6ZdNvlx5oDipS0EukAZNbvDNUG2epW-ijS8G_Ii8GPUV8fbpb8uPqy_fLm-L22_XXy4vbwgjBUtFrwUFgNwDXAplsrBw4g17qtqq07bmxXANwbm3TdbKWou5Yq2Vf1W0vLa-25P0xdxf8rz3GpGYX19f1gn4fVdtWUDGez5a8e1LZNG0ujK1CfhSa4GMMOKhdcLMOB8VArVDUqFYoaoWiGFMZSja9PaXv-xntP8uJQhZ8PAowt_HoMKhoHC4GrQtokrLePZ3_6T-7yWyc0dNPPGAc_T4suWfFVOQK1N06i3UV0AIwqGX1F6k_spM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66809118</pqid></control><display><type>article</type><title>Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Zeng, Yi ; Yang, Zhong ; Xu, Jian-Guo ; Yang, Meng-Su ; Zeng, Zhi-Xiong ; You, Chao</creator><creatorcontrib>Zeng, Yi ; Yang, Zhong ; Xu, Jian-Guo ; Yang, Meng-Su ; Zeng, Zhi-Xiong ; You, Chao</creatorcontrib><description>Abstract Morphology, immunocytochemistry, growth curve assay, and flow cytometry were used to investigate the effects of all-trans retinoic acid (RA) on cell proliferation, cell cycle progression and differentiation of the astrocytoma cell line SHG-44 from glioblastoma multiforme (World Health Organization grade IV). The differentially expressed genes from RA-treated and normal SHG-44 were identified by cDNA microarray after the cell line SHG-44 was treated with 10 μM RA for 3 days. Validation of some differentially expressed genes was performed by Northern Blot analysis. The expression of glial fibrillary acidic protein (GFAP) was markedly increased in RA-treated SHG-44 cells. Other changes included a short shuttle shape, small nucleus, decreased karyoplasm proportion, the formation of increased thin cytoplasmic processes, reduced cell growth and a 15% increase in G0/G1 phase cell populations. In addition, 42 known genes were identified with altered expression in our cDNA microarray. There was stable down-regulation of MDM2 and UGB as well as overexpression of SOD2, CSTB, and G3BP when RA-treated SHG-44 was compared with normal SHG-44. RA simultaneously suppressed the proliferation of SHG-44 cells significantly as well as induced differentiation and altered gene expression.</description><identifier>ISSN: 0967-5868</identifier><identifier>EISSN: 1532-2653</identifier><identifier>DOI: 10.1016/j.jocn.2007.11.014</identifier><identifier>PMID: 19091570</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Antineoplastic Agents - pharmacology ; Astrocytoma ; Astrocytoma cells ; cDNA microarray ; Cell cycle ; Cell Cycle - drug effects ; Cell Differentiation - drug effects ; Cell Line, Tumor ; Cell proliferation ; Cell Proliferation - drug effects ; Cytology ; Differentiation ; DNA microarrays ; Flow cytometry ; Flow Cytometry - methods ; G1 phase ; Gene Expression Regulation - drug effects ; Glial fibrillary acidic protein ; Glial Fibrillary Acidic Protein - metabolism ; Glioblastoma - pathology ; Glioblastoma - physiopathology ; glioblastoma cells ; glioblastoma multiforme ; Growth curves ; Humans ; Immunocytochemistry ; MDM2 protein ; Nervous system ; Neurology ; Oligonucleotide Array Sequence Analysis - methods ; Proliferation ; Retinoic acid ; Retinoic acid (RA) ; SHG-44 ; Superoxide dismutase ; Tretinoin - pharmacology</subject><ispartof>Journal of clinical neuroscience, 2009-02, Vol.16 (2), p.285-294</ispartof><rights>2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-ba4204e9f02a4e176d7f210b7a833adb2cd2a0022dd69975745918a7b358b7d23</citedby><cites>FETCH-LOGICAL-c441t-ba4204e9f02a4e176d7f210b7a833adb2cd2a0022dd69975745918a7b358b7d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0967586808001057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19091570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Yi</creatorcontrib><creatorcontrib>Yang, Zhong</creatorcontrib><creatorcontrib>Xu, Jian-Guo</creatorcontrib><creatorcontrib>Yang, Meng-Su</creatorcontrib><creatorcontrib>Zeng, Zhi-Xiong</creatorcontrib><creatorcontrib>You, Chao</creatorcontrib><title>Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro</title><title>Journal of clinical neuroscience</title><addtitle>J Clin Neurosci</addtitle><description>Abstract Morphology, immunocytochemistry, growth curve assay, and flow cytometry were used to investigate the effects of all-trans retinoic acid (RA) on cell proliferation, cell cycle progression and differentiation of the astrocytoma cell line SHG-44 from glioblastoma multiforme (World Health Organization grade IV). The differentially expressed genes from RA-treated and normal SHG-44 were identified by cDNA microarray after the cell line SHG-44 was treated with 10 μM RA for 3 days. Validation of some differentially expressed genes was performed by Northern Blot analysis. The expression of glial fibrillary acidic protein (GFAP) was markedly increased in RA-treated SHG-44 cells. Other changes included a short shuttle shape, small nucleus, decreased karyoplasm proportion, the formation of increased thin cytoplasmic processes, reduced cell growth and a 15% increase in G0/G1 phase cell populations. In addition, 42 known genes were identified with altered expression in our cDNA microarray. There was stable down-regulation of MDM2 and UGB as well as overexpression of SOD2, CSTB, and G3BP when RA-treated SHG-44 was compared with normal SHG-44. RA simultaneously suppressed the proliferation of SHG-44 cells significantly as well as induced differentiation and altered gene expression.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Astrocytoma</subject><subject>Astrocytoma cells</subject><subject>cDNA microarray</subject><subject>Cell cycle</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cytology</subject><subject>Differentiation</subject><subject>DNA microarrays</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>G1 phase</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glial fibrillary acidic protein</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Glioblastoma - pathology</subject><subject>Glioblastoma - physiopathology</subject><subject>glioblastoma cells</subject><subject>glioblastoma multiforme</subject><subject>Growth curves</subject><subject>Humans</subject><subject>Immunocytochemistry</subject><subject>MDM2 protein</subject><subject>Nervous system</subject><subject>Neurology</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Proliferation</subject><subject>Retinoic acid</subject><subject>Retinoic acid (RA)</subject><subject>SHG-44</subject><subject>Superoxide dismutase</subject><subject>Tretinoin - pharmacology</subject><issn>0967-5868</issn><issn>1532-2653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kkFv1DAUhC0EotuFP8AB-QSnhGfHiRMJIVWFtkiVOBTOlmO_tA5JvNjewv57HO1KSBx6epeZ0dN8Q8gbBiUD1nwYy9GbpeQAsmSsBCaekQ2rK17wpq6ekw10jSzqtmnPyHmMIwB0ooKX5Ix10LFawobEz24YMOCSnJ6mA8U_u4AxoqX3uGCkQ_AzTQ9I7yfn-0nH5GdNDU4TndyC9O7muhCCpoA6ZdNvlx5oDipS0EukAZNbvDNUG2epW-ijS8G_Ii8GPUV8fbpb8uPqy_fLm-L22_XXy4vbwgjBUtFrwUFgNwDXAplsrBw4g17qtqq07bmxXANwbm3TdbKWou5Yq2Vf1W0vLa-25P0xdxf8rz3GpGYX19f1gn4fVdtWUDGez5a8e1LZNG0ujK1CfhSa4GMMOKhdcLMOB8VArVDUqFYoaoWiGFMZSja9PaXv-xntP8uJQhZ8PAowt_HoMKhoHC4GrQtokrLePZ3_6T-7yWyc0dNPPGAc_T4suWfFVOQK1N06i3UV0AIwqGX1F6k_spM</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Zeng, Yi</creator><creator>Yang, Zhong</creator><creator>Xu, Jian-Guo</creator><creator>Yang, Meng-Su</creator><creator>Zeng, Zhi-Xiong</creator><creator>You, Chao</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20090201</creationdate><title>Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro</title><author>Zeng, Yi ; Yang, Zhong ; Xu, Jian-Guo ; Yang, Meng-Su ; Zeng, Zhi-Xiong ; You, Chao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-ba4204e9f02a4e176d7f210b7a833adb2cd2a0022dd69975745918a7b358b7d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Astrocytoma</topic><topic>Astrocytoma cells</topic><topic>cDNA microarray</topic><topic>Cell cycle</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cytology</topic><topic>Differentiation</topic><topic>DNA microarrays</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>G1 phase</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glial fibrillary acidic protein</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Glioblastoma - pathology</topic><topic>Glioblastoma - physiopathology</topic><topic>glioblastoma cells</topic><topic>glioblastoma multiforme</topic><topic>Growth curves</topic><topic>Humans</topic><topic>Immunocytochemistry</topic><topic>MDM2 protein</topic><topic>Nervous system</topic><topic>Neurology</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Proliferation</topic><topic>Retinoic acid</topic><topic>Retinoic acid (RA)</topic><topic>SHG-44</topic><topic>Superoxide dismutase</topic><topic>Tretinoin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Yi</creatorcontrib><creatorcontrib>Yang, Zhong</creatorcontrib><creatorcontrib>Xu, Jian-Guo</creatorcontrib><creatorcontrib>Yang, Meng-Su</creatorcontrib><creatorcontrib>Zeng, Zhi-Xiong</creatorcontrib><creatorcontrib>You, Chao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of clinical neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Yi</au><au>Yang, Zhong</au><au>Xu, Jian-Guo</au><au>Yang, Meng-Su</au><au>Zeng, Zhi-Xiong</au><au>You, Chao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro</atitle><jtitle>Journal of clinical neuroscience</jtitle><addtitle>J Clin Neurosci</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>16</volume><issue>2</issue><spage>285</spage><epage>294</epage><pages>285-294</pages><issn>0967-5868</issn><eissn>1532-2653</eissn><abstract>Abstract Morphology, immunocytochemistry, growth curve assay, and flow cytometry were used to investigate the effects of all-trans retinoic acid (RA) on cell proliferation, cell cycle progression and differentiation of the astrocytoma cell line SHG-44 from glioblastoma multiforme (World Health Organization grade IV). The differentially expressed genes from RA-treated and normal SHG-44 were identified by cDNA microarray after the cell line SHG-44 was treated with 10 μM RA for 3 days. Validation of some differentially expressed genes was performed by Northern Blot analysis. The expression of glial fibrillary acidic protein (GFAP) was markedly increased in RA-treated SHG-44 cells. Other changes included a short shuttle shape, small nucleus, decreased karyoplasm proportion, the formation of increased thin cytoplasmic processes, reduced cell growth and a 15% increase in G0/G1 phase cell populations. In addition, 42 known genes were identified with altered expression in our cDNA microarray. There was stable down-regulation of MDM2 and UGB as well as overexpression of SOD2, CSTB, and G3BP when RA-treated SHG-44 was compared with normal SHG-44. RA simultaneously suppressed the proliferation of SHG-44 cells significantly as well as induced differentiation and altered gene expression.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>19091570</pmid><doi>10.1016/j.jocn.2007.11.014</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0967-5868 |
| ispartof | Journal of clinical neuroscience, 2009-02, Vol.16 (2), p.285-294 |
| issn | 0967-5868 1532-2653 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_883031288 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antineoplastic Agents - pharmacology Astrocytoma Astrocytoma cells cDNA microarray Cell cycle Cell Cycle - drug effects Cell Differentiation - drug effects Cell Line, Tumor Cell proliferation Cell Proliferation - drug effects Cytology Differentiation DNA microarrays Flow cytometry Flow Cytometry - methods G1 phase Gene Expression Regulation - drug effects Glial fibrillary acidic protein Glial Fibrillary Acidic Protein - metabolism Glioblastoma - pathology Glioblastoma - physiopathology glioblastoma cells glioblastoma multiforme Growth curves Humans Immunocytochemistry MDM2 protein Nervous system Neurology Oligonucleotide Array Sequence Analysis - methods Proliferation Retinoic acid Retinoic acid (RA) SHG-44 Superoxide dismutase Tretinoin - pharmacology |
| title | Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T17%3A12%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differentially%20expressed%20genes%20from%20the%20glioblastoma%20cell%20line%20SHG-44%20treated%20with%20all-trans%20retinoic%20acid%20in%20vitro&rft.jtitle=Journal%20of%20clinical%20neuroscience&rft.au=Zeng,%20Yi&rft.date=2009-02-01&rft.volume=16&rft.issue=2&rft.spage=285&rft.epage=294&rft.pages=285-294&rft.issn=0967-5868&rft.eissn=1532-2653&rft_id=info:doi/10.1016/j.jocn.2007.11.014&rft_dat=%3Cproquest_cross%3E66809118%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66809118&rft_id=info:pmid/19091570&rft_els_id=1_s2_0_S0967586808001057&rfr_iscdi=true |