Triggered release of small molecules from proteinoid microspheres

Proteinoid microspheres (PM) are formed by the thermal condensation of amino acids. They have been useful to further evolutionary theory, as catalysts for some biochemical reactions, but they have not been overly useful as controlled delivery agents. It is possible however to construct PMs that contain organic small molecules in the interior space. This means that a PM could be used as a delivery agent, if a suitable method could be discovered to cause the release of the internal material. This report describes the formation of a PM that includes a molecular bridging agent that can be removed in a reducing environment. Removal of the bridge opens a hole or window in the PM that allows the interior material to escape. The rate at which the interior material is released from the PM can be controlled by the size of the window or by the reduction potential in the environment. These PMs can be used to temporally treat a variety of complications including wounds (chronic or acute) by delivering a sequestered reagent in a controlled manner and are advantageous in that amino acids are the primary delivery vehicle breakdown product. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009