Functionalization of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds via surface heparinization for bone tissue engineering
Scaffolds exhibiting biological recognition and specificity play an important role in tissue engineering and regenerative medicine. The bioactivity of scaffolds in turn influences, directs, or manipulates cellular responses. In this study, chitosan/poly(lactic acid‐co‐glycolic acid) (chitosan/PLAGA)...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2010-06, Vol.93A (3), p.1193-1208 |
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| container_title | Journal of biomedical materials research. Part A |
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| creator | Jiang, Tao Khan, Yusuf Nair, Lakshmi S. Abdel-Fattah, Wafa I. Laurencin, Cato T. |
| description | Scaffolds exhibiting biological recognition and specificity play an important role in tissue engineering and regenerative medicine. The bioactivity of scaffolds in turn influences, directs, or manipulates cellular responses. In this study, chitosan/poly(lactic acid‐co‐glycolic acid) (chitosan/PLAGA) sintered microsphere scaffolds were functionalized via heparin immobilization. Heparin was successfully immobilized on chitosan/PLAGA scaffolds with controllable loading efficiency. Mechanical testing showed that heparinization of chitosan/PLAGA scaffolds did not significantly alter the mechanical properties and porous structures. In addition, the heparinized chitosan/PLAGA scaffolds possessed a compressive modulus of 403.98 ± 19.53 MPa and a compressive strength of 9.83 ± 0.94 MPa, which are in the range of human trabecular bone. Furthermore, the heparinized chitosan/PLAGA scaffolds had an interconnected porous structure with a total pore volume of 30.93 ± 0.90% and a median pore size of 172.33 ± 5.89 μm. The effect of immobilized heparin on osteoblast‐like MC3T3‐E1 cell growth was investigated. MC3T3‐E1 cells proliferated three dimensionally throughout the porous structure of the scaffolds. Heparinized chitosan/PLAGA scaffolds with low heparin loading (1.7 μg/scaffold) were shown to be capable of stimulating MC3T3‐E1 cell proliferation by MTS assay and cell differentiation as evidenced by elevated osteocalcin expression when compared with nonheparinized chitosan/PLAGA scaffold and chitosan/PLAGA scaffold with high heparin loading (14.1 μg/scaffold). This study demonstrated the potential of functionalizing chitosan/PLAGA scaffolds via heparinization with improved cell functions for bone tissue engineering applications. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010 |
| doi_str_mv | 10.1002/jbm.a.32615 |
| format | Article |
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The bioactivity of scaffolds in turn influences, directs, or manipulates cellular responses. In this study, chitosan/poly(lactic acid‐co‐glycolic acid) (chitosan/PLAGA) sintered microsphere scaffolds were functionalized via heparin immobilization. Heparin was successfully immobilized on chitosan/PLAGA scaffolds with controllable loading efficiency. Mechanical testing showed that heparinization of chitosan/PLAGA scaffolds did not significantly alter the mechanical properties and porous structures. In addition, the heparinized chitosan/PLAGA scaffolds possessed a compressive modulus of 403.98 ± 19.53 MPa and a compressive strength of 9.83 ± 0.94 MPa, which are in the range of human trabecular bone. Furthermore, the heparinized chitosan/PLAGA scaffolds had an interconnected porous structure with a total pore volume of 30.93 ± 0.90% and a median pore size of 172.33 ± 5.89 μm. The effect of immobilized heparin on osteoblast‐like MC3T3‐E1 cell growth was investigated. MC3T3‐E1 cells proliferated three dimensionally throughout the porous structure of the scaffolds. Heparinized chitosan/PLAGA scaffolds with low heparin loading (1.7 μg/scaffold) were shown to be capable of stimulating MC3T3‐E1 cell proliferation by MTS assay and cell differentiation as evidenced by elevated osteocalcin expression when compared with nonheparinized chitosan/PLAGA scaffold and chitosan/PLAGA scaffold with high heparin loading (14.1 μg/scaffold). This study demonstrated the potential of functionalizing chitosan/PLAGA scaffolds via heparinization with improved cell functions for bone tissue engineering applications. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010</description><identifier>ISSN: 1549-3296</identifier><identifier>ISSN: 1552-4965</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.32615</identifier><identifier>PMID: 19777575</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adsorption - drug effects ; Alkaline Phosphatase - metabolism ; Animals ; bioactive scaffold ; Biological and medical sciences ; Biotechnology ; Bone and Bones - drug effects ; bone tissue engineering ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; chitosan ; Chitosan - chemistry ; Chitosan - pharmacology ; Compressive Strength - drug effects ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; heparin ; Heparin - chemistry ; Heparin - pharmacology ; Immobilized Proteins - pharmacology ; Industrial applications and implications. Economical aspects ; Lactic Acid - chemistry ; Lactic Acid - pharmacology ; Medical sciences ; Mice ; Microscopy, Electron, Scanning ; Microspheres ; Miscellaneous ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - enzymology ; Osteocalcin - metabolism ; Photoelectron Spectroscopy ; poly(lactic acid-glycolic acid) ; Polyglycolic Acid - chemistry ; Polyglycolic Acid - pharmacology ; Porosity - drug effects ; Solubility - drug effects ; Surface Properties - drug effects ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Scaffolds exhibiting biological recognition and specificity play an important role in tissue engineering and regenerative medicine. The bioactivity of scaffolds in turn influences, directs, or manipulates cellular responses. In this study, chitosan/poly(lactic acid‐co‐glycolic acid) (chitosan/PLAGA) sintered microsphere scaffolds were functionalized via heparin immobilization. Heparin was successfully immobilized on chitosan/PLAGA scaffolds with controllable loading efficiency. Mechanical testing showed that heparinization of chitosan/PLAGA scaffolds did not significantly alter the mechanical properties and porous structures. In addition, the heparinized chitosan/PLAGA scaffolds possessed a compressive modulus of 403.98 ± 19.53 MPa and a compressive strength of 9.83 ± 0.94 MPa, which are in the range of human trabecular bone. Furthermore, the heparinized chitosan/PLAGA scaffolds had an interconnected porous structure with a total pore volume of 30.93 ± 0.90% and a median pore size of 172.33 ± 5.89 μm. The effect of immobilized heparin on osteoblast‐like MC3T3‐E1 cell growth was investigated. MC3T3‐E1 cells proliferated three dimensionally throughout the porous structure of the scaffolds. Heparinized chitosan/PLAGA scaffolds with low heparin loading (1.7 μg/scaffold) were shown to be capable of stimulating MC3T3‐E1 cell proliferation by MTS assay and cell differentiation as evidenced by elevated osteocalcin expression when compared with nonheparinized chitosan/PLAGA scaffold and chitosan/PLAGA scaffold with high heparin loading (14.1 μg/scaffold). This study demonstrated the potential of functionalizing chitosan/PLAGA scaffolds via heparinization with improved cell functions for bone tissue engineering applications. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010</description><subject>Adsorption - drug effects</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>bioactive scaffold</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Bone and Bones - drug effects</subject><subject>bone tissue engineering</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>Compressive Strength - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>heparin</subject><subject>Heparin - chemistry</subject><subject>Heparin - pharmacology</subject><subject>Immobilized Proteins - pharmacology</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Lactic Acid - chemistry</subject><subject>Lactic Acid - pharmacology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microspheres</subject><subject>Miscellaneous</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - enzymology</subject><subject>Osteocalcin - metabolism</subject><subject>Photoelectron Spectroscopy</subject><subject>poly(lactic acid-glycolic acid)</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Polyglycolic Acid - pharmacology</subject><subject>Porosity - drug effects</subject><subject>Solubility - drug effects</subject><subject>Surface Properties - drug effects</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><issn>1549-3296</issn><issn>1552-4965</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UtvEzEQAOAVAtEHnLgjXxAgtOn6HR9L1BZoKRIqQuJieb3jxMVZB3sXCH-DP4xD0nKjJ89I38zIM1X1BDcT3DTk6LpdTsyEEoH5vWofc05qpgS_v4mZqilRYq86yPm6YNFw8rDaw0pKySXfr36fjr0dfOxN8L_MJkDRIbvwQ8ymP1rFsH4RTBEWGeu7eh7WNoZd9hJl3w-QoENLb1PMq0VJULbGuRi6jL57g_KYnLGAFrAyyfc3U1xMqI09oMHnPAKCfu57gCLmj6oHzoQMj3fvYfXp9ORq9qa--HD2dnZ8UVsmCK8NF8Bc-alsnXVTO6USHHdAOi4aJg1lmHWWt1a0uKNgu1YxglssZSs7jjE9rJ5v-65S_DZCHvTSZwshmB7imPV0ShvChVR3S6G4YkqSO6WkVDCBFSvy1VZuFpcTOL1KfmnSWuNGbw6ry2G10X8PW_TTXd-xXUL3z-4uWcCzHTBl_8El01ufbx0hUjaNoMXhrfvhA6z_N1O_e_3-Zni9rfF5gJ-3NSZ91UJSyfXnyzN9qb58vJqdS31O_wBRms3r</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Jiang, Tao</creator><creator>Khan, Yusuf</creator><creator>Nair, Lakshmi S.</creator><creator>Abdel-Fattah, Wafa I.</creator><creator>Laurencin, Cato T.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7QP</scope></search><sort><creationdate>20100601</creationdate><title>Functionalization of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds via surface heparinization for bone tissue engineering</title><author>Jiang, Tao ; Khan, Yusuf ; Nair, Lakshmi S. ; Abdel-Fattah, Wafa I. ; Laurencin, Cato T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4625-a56e4f9657bfcf8c837ef5fe2d56047a3414dc5bc6b1d3ecdb9421b177b7d5113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adsorption - drug effects</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>bioactive scaffold</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Bone and Bones - drug effects</topic><topic>bone tissue engineering</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - pharmacology</topic><topic>Compressive Strength - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>heparin</topic><topic>Heparin - chemistry</topic><topic>Heparin - pharmacology</topic><topic>Immobilized Proteins - pharmacology</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Lactic Acid - chemistry</topic><topic>Lactic Acid - pharmacology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microspheres</topic><topic>Miscellaneous</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - enzymology</topic><topic>Osteocalcin - metabolism</topic><topic>Photoelectron Spectroscopy</topic><topic>poly(lactic acid-glycolic acid)</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polyglycolic Acid - pharmacology</topic><topic>Porosity - drug effects</topic><topic>Solubility - drug effects</topic><topic>Surface Properties - drug effects</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Tao</au><au>Khan, Yusuf</au><au>Nair, Lakshmi S.</au><au>Abdel-Fattah, Wafa I.</au><au>Laurencin, Cato T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functionalization of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds via surface heparinization for bone tissue engineering</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>93A</volume><issue>3</issue><spage>1193</spage><epage>1208</epage><pages>1193-1208</pages><issn>1549-3296</issn><issn>1552-4965</issn><eissn>1552-4965</eissn><abstract>Scaffolds exhibiting biological recognition and specificity play an important role in tissue engineering and regenerative medicine. The bioactivity of scaffolds in turn influences, directs, or manipulates cellular responses. In this study, chitosan/poly(lactic acid‐co‐glycolic acid) (chitosan/PLAGA) sintered microsphere scaffolds were functionalized via heparin immobilization. Heparin was successfully immobilized on chitosan/PLAGA scaffolds with controllable loading efficiency. Mechanical testing showed that heparinization of chitosan/PLAGA scaffolds did not significantly alter the mechanical properties and porous structures. In addition, the heparinized chitosan/PLAGA scaffolds possessed a compressive modulus of 403.98 ± 19.53 MPa and a compressive strength of 9.83 ± 0.94 MPa, which are in the range of human trabecular bone. Furthermore, the heparinized chitosan/PLAGA scaffolds had an interconnected porous structure with a total pore volume of 30.93 ± 0.90% and a median pore size of 172.33 ± 5.89 μm. The effect of immobilized heparin on osteoblast‐like MC3T3‐E1 cell growth was investigated. MC3T3‐E1 cells proliferated three dimensionally throughout the porous structure of the scaffolds. Heparinized chitosan/PLAGA scaffolds with low heparin loading (1.7 μg/scaffold) were shown to be capable of stimulating MC3T3‐E1 cell proliferation by MTS assay and cell differentiation as evidenced by elevated osteocalcin expression when compared with nonheparinized chitosan/PLAGA scaffold and chitosan/PLAGA scaffold with high heparin loading (14.1 μg/scaffold). This study demonstrated the potential of functionalizing chitosan/PLAGA scaffolds via heparinization with improved cell functions for bone tissue engineering applications. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19777575</pmid><doi>10.1002/jbm.a.32615</doi><tpages>16</tpages></addata></record> |
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| ispartof | Journal of biomedical materials research. Part A, 2010-06, Vol.93A (3), p.1193-1208 |
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| subjects | Adsorption - drug effects Alkaline Phosphatase - metabolism Animals bioactive scaffold Biological and medical sciences Biotechnology Bone and Bones - drug effects bone tissue engineering Cell Proliferation - drug effects Cell Survival - drug effects chitosan Chitosan - chemistry Chitosan - pharmacology Compressive Strength - drug effects Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry heparin Heparin - chemistry Heparin - pharmacology Immobilized Proteins - pharmacology Industrial applications and implications. Economical aspects Lactic Acid - chemistry Lactic Acid - pharmacology Medical sciences Mice Microscopy, Electron, Scanning Microspheres Miscellaneous Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - enzymology Osteocalcin - metabolism Photoelectron Spectroscopy poly(lactic acid-glycolic acid) Polyglycolic Acid - chemistry Polyglycolic Acid - pharmacology Porosity - drug effects Solubility - drug effects Surface Properties - drug effects Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Tissue Engineering - methods Tissue Scaffolds - chemistry |
| title | Functionalization of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds via surface heparinization for bone tissue engineering |
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