Growth factor delivery through electrospun nanofibers in scaffolds for tissue engineering applications
Tissue engineering scaffolds should ideally mimic the natural ECM in structure and function. Electrospun nanofibrous scaffolds are easily fabricated and possess a biomimetic nanostructure. Scaffolds can mimic ECM function by acting as a depot for sustained release of growth factors. bFGF, an importa...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2010-06, Vol.93A (4), p.1539-1550 |
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| creator | Sahoo, Sambit Ang, Lay Teng Goh, James Cho-Hong Toh, Siew-Lok |
| description | Tissue engineering scaffolds should ideally mimic the natural ECM in structure and function. Electrospun nanofibrous scaffolds are easily fabricated and possess a biomimetic nanostructure. Scaffolds can mimic ECM function by acting as a depot for sustained release of growth factors. bFGF, an important growth factor involved in tissue repair and mesenchymal stem cell proliferation and differentiation, is a suitable candidate for sustained delivery from scaffolds. In this study, we present two types of PLGA nanofibers incorporated with bFGF, fabricated using the facile technique of blending and electrospinning (Group I) and by the more complex technique of coaxial electrospinning (Group II). bFGF was randomly dispersed in Group I and distributed as a central core within Group II nanofibers; both scaffolds showed similar protein encapsulation efficiency and release over 1–2 weeks. Although both scaffold groups favored bone marrow stem cell attachment and subsequent proliferation, cells cultured on Group I scaffolds demonstrated increased collagen production and upregulated gene expression of specific ECM proteins, indicating fibroblastic differentiation. The study shows that the electrospinning technique could be used to prolong growth factor release from scaffolds and an appropriately sustained growth factor release profile in combination with a nanofibrous substrate could positively influence stem cell behavior and fate. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010 |
| doi_str_mv | 10.1002/jbm.a.32645 |
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Electrospun nanofibrous scaffolds are easily fabricated and possess a biomimetic nanostructure. Scaffolds can mimic ECM function by acting as a depot for sustained release of growth factors. bFGF, an important growth factor involved in tissue repair and mesenchymal stem cell proliferation and differentiation, is a suitable candidate for sustained delivery from scaffolds. In this study, we present two types of PLGA nanofibers incorporated with bFGF, fabricated using the facile technique of blending and electrospinning (Group I) and by the more complex technique of coaxial electrospinning (Group II). bFGF was randomly dispersed in Group I and distributed as a central core within Group II nanofibers; both scaffolds showed similar protein encapsulation efficiency and release over 1–2 weeks. Although both scaffold groups favored bone marrow stem cell attachment and subsequent proliferation, cells cultured on Group I scaffolds demonstrated increased collagen production and upregulated gene expression of specific ECM proteins, indicating fibroblastic differentiation. The study shows that the electrospinning technique could be used to prolong growth factor release from scaffolds and an appropriately sustained growth factor release profile in combination with a nanofibrous substrate could positively influence stem cell behavior and fate. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010</description><identifier>ISSN: 1549-3296</identifier><identifier>ISSN: 1552-4965</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.32645</identifier><identifier>PMID: 20014288</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; biomimetic scaffolds ; Biomimetics ; Biotechnology ; Cell Proliferation ; Collagen - chemistry ; Electrochemistry - methods ; electrospinning ; Extracellular Matrix - metabolism ; Fibroblast Growth Factor 2 - chemistry ; Fundamental and applied biological sciences. Psychology ; growth factors ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Kinetics ; Medical sciences ; Microscopy, Electron, Scanning - methods ; Miscellaneous ; nanofibers ; Nanofibers - chemistry ; Nanotechnology - methods ; Rabbits ; Reverse Transcriptase Polymerase Chain Reaction ; Stem Cells - cytology ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; tissue engineering ; Tissue Engineering - methods</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Tissue engineering scaffolds should ideally mimic the natural ECM in structure and function. Electrospun nanofibrous scaffolds are easily fabricated and possess a biomimetic nanostructure. Scaffolds can mimic ECM function by acting as a depot for sustained release of growth factors. bFGF, an important growth factor involved in tissue repair and mesenchymal stem cell proliferation and differentiation, is a suitable candidate for sustained delivery from scaffolds. In this study, we present two types of PLGA nanofibers incorporated with bFGF, fabricated using the facile technique of blending and electrospinning (Group I) and by the more complex technique of coaxial electrospinning (Group II). bFGF was randomly dispersed in Group I and distributed as a central core within Group II nanofibers; both scaffolds showed similar protein encapsulation efficiency and release over 1–2 weeks. Although both scaffold groups favored bone marrow stem cell attachment and subsequent proliferation, cells cultured on Group I scaffolds demonstrated increased collagen production and upregulated gene expression of specific ECM proteins, indicating fibroblastic differentiation. The study shows that the electrospinning technique could be used to prolong growth factor release from scaffolds and an appropriately sustained growth factor release profile in combination with a nanofibrous substrate could positively influence stem cell behavior and fate. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>biomimetic scaffolds</subject><subject>Biomimetics</subject><subject>Biotechnology</subject><subject>Cell Proliferation</subject><subject>Collagen - chemistry</subject><subject>Electrochemistry - methods</subject><subject>electrospinning</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibroblast Growth Factor 2 - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>growth factors</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Miscellaneous</subject><subject>nanofibers</subject><subject>Nanofibers - chemistry</subject><subject>Nanotechnology - methods</subject><subject>Rabbits</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Stem Cells - cytology</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1549-3296</issn><issn>1552-4965</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v00AQxS0EoqVw4o72gjggp95Pr4-0ommrUIQUhMRlNbZnky3O2uzalPz3bJu03NrTjDS_N096L8ve0mJGi4IdX9ebGcw4U0I-yw6plCwXlZLPb3dR5ZxV6iB7FeN1glUh2cvsgBUFFUzrw8zOQ38zromFZuwDabFzfzBsybgO_bRaE-ywGUMfh8kTD763rsYQifMkNmBt37WR2CQcXYwTEvQr5xGD8ysCw9C5BkbX-_g6e2Ghi_hmP4-y72efl6fn-eLr_OL00yJvJNMyVyAVtQJ0rdoaeNkKjrUQdbqB1kgrWUJpOQAXnFKuk0ihrupG2dYKXvOj7MPu7xD63xPG0WxcbLDrwGM_RaM1L5hkUjxNqmQmBK2eJEvOKyoKzRL5cUc2KbEY0JohuA2EraGFue3KpK4MmLuuEv1u_3eqN9g-sPflJOD9HoAUdmcD-MbF_xzTtKzubOmOu3Edbh_zNJcnX-7N853GxRH_Pmgg_DKq5KU0P67mZvFNLs-XP6_MGf8HHIq8jw</recordid><startdate>20100615</startdate><enddate>20100615</enddate><creator>Sahoo, Sambit</creator><creator>Ang, Lay Teng</creator><creator>Goh, James Cho-Hong</creator><creator>Toh, Siew-Lok</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100615</creationdate><title>Growth factor delivery through electrospun nanofibers in scaffolds for tissue engineering applications</title><author>Sahoo, Sambit ; Ang, Lay Teng ; Goh, James Cho-Hong ; Toh, Siew-Lok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5285-6a561f4a8b6dba37d43eb44b285a88e1957a7f3aa34311385286e89bc6fdf43b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>biomimetic scaffolds</topic><topic>Biomimetics</topic><topic>Biotechnology</topic><topic>Cell Proliferation</topic><topic>Collagen - chemistry</topic><topic>Electrochemistry - methods</topic><topic>electrospinning</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibroblast Growth Factor 2 - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>growth factors</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Miscellaneous</topic><topic>nanofibers</topic><topic>Nanofibers - chemistry</topic><topic>Nanotechnology - methods</topic><topic>Rabbits</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Stem Cells - cytology</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. 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Scaffolds can mimic ECM function by acting as a depot for sustained release of growth factors. bFGF, an important growth factor involved in tissue repair and mesenchymal stem cell proliferation and differentiation, is a suitable candidate for sustained delivery from scaffolds. In this study, we present two types of PLGA nanofibers incorporated with bFGF, fabricated using the facile technique of blending and electrospinning (Group I) and by the more complex technique of coaxial electrospinning (Group II). bFGF was randomly dispersed in Group I and distributed as a central core within Group II nanofibers; both scaffolds showed similar protein encapsulation efficiency and release over 1–2 weeks. Although both scaffold groups favored bone marrow stem cell attachment and subsequent proliferation, cells cultured on Group I scaffolds demonstrated increased collagen production and upregulated gene expression of specific ECM proteins, indicating fibroblastic differentiation. 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| subjects | Animals Biological and medical sciences biomimetic scaffolds Biomimetics Biotechnology Cell Proliferation Collagen - chemistry Electrochemistry - methods electrospinning Extracellular Matrix - metabolism Fibroblast Growth Factor 2 - chemistry Fundamental and applied biological sciences. Psychology growth factors Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Kinetics Medical sciences Microscopy, Electron, Scanning - methods Miscellaneous nanofibers Nanofibers - chemistry Nanotechnology - methods Rabbits Reverse Transcriptase Polymerase Chain Reaction Stem Cells - cytology Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments tissue engineering Tissue Engineering - methods |
| title | Growth factor delivery through electrospun nanofibers in scaffolds for tissue engineering applications |
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