A model for studying human articular cartilage integration in vitro
One of the major obstacles hindering cartilage repair is the integration of the reparative cartilage with the recipient cartilage. The purpose of this study was to develop an in vitro model that can be conveniently applied to simulate and improve the integration of tissue engineered cartilage with n...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2010-08, Vol.94A (2), p.509-514 |
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| container_title | Journal of biomedical materials research. Part A |
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| creator | Enders, J. Tyler Otto, Thomas J. Peters, H. Charlie Wu, Jin Hardouin, Scott Moed, Berton R. Zhang, Zijun |
| description | One of the major obstacles hindering cartilage repair is the integration of the reparative cartilage with the recipient cartilage. The purpose of this study was to develop an in
vitro model that can be conveniently applied to simulate and improve the integration of tissue engineered cartilage with native articular cartilage. This model, a cartilage integration construct, consists of a cartilage explant and isolated chondrocytes. The explant was anchored to agarose gel on a culture plate as agarose gelation at 4°C to seal the gap between the bottom of the explant and culture plate surface. Isolated chondrocytes were added and confined in the defect created in the center of the explant. After 4 weeks of culture, neocartilage containing proteoglycans and type II collagen was formed. Minimal integration occurred between the neocartilage and the cartilage explant, resembling the failure of cartilage integration manifested in experimental and clinical cartilage repair. In this model, agarose gel anchors the explant onto culture plate by altering temperatures and effectively prevents “leakage” of the isolated chondrocytes from the defect of the explant. This model provides a convenient simulation of the cartilage integration process in vitro and has applications in studies of cartilage integration and cartilage tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res part A, 2010 |
| doi_str_mv | 10.1002/jbm.a.32719 |
| format | Article |
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vitro model that can be conveniently applied to simulate and improve the integration of tissue engineered cartilage with native articular cartilage. This model, a cartilage integration construct, consists of a cartilage explant and isolated chondrocytes. The explant was anchored to agarose gel on a culture plate as agarose gelation at 4°C to seal the gap between the bottom of the explant and culture plate surface. Isolated chondrocytes were added and confined in the defect created in the center of the explant. After 4 weeks of culture, neocartilage containing proteoglycans and type II collagen was formed. Minimal integration occurred between the neocartilage and the cartilage explant, resembling the failure of cartilage integration manifested in experimental and clinical cartilage repair. In this model, agarose gel anchors the explant onto culture plate by altering temperatures and effectively prevents “leakage” of the isolated chondrocytes from the defect of the explant. This model provides a convenient simulation of the cartilage integration process in vitro and has applications in studies of cartilage integration and cartilage tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res part A, 2010</description><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cartilage</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - physiology</subject><subject>Cells, Cultured</subject><subject>chondrocytes</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - metabolism</subject><subject>Computerized, statistical medical data processing and models in biomedicine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>Humans</subject><subject>in vitro</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>integration</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Models and simulation</subject><subject>Models, Biological</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tissue Culture Techniques</subject><subject>tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1549-3296</issn><issn>1552-4965</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0btP5DAQB2ALHQIOqOhRmhPFKYtf8aOEFcdDC0iIR2lNHGcx5AF2crD_PVl2gQ4q_4pvZqwZhHYIHhGM6f5DXo9gxKgkegVtkCyjKdci-zXPXKeMarGOfsf4MGCBM7qG1ikmSkihN9D4IKnbwlVJ2YYkdn0x8800ue9raBIInbd9BSGx81jB1CW-6dw0QOfbZsjJf9-FdgutllBFt718N9HNv6Pr8Uk6uTw-HR9MUssF1SkhuCgyqjUplHVYEcscsTnjyuYCci3BKiqELEEwqzkoSwsrNJQCiMolZ5tob9H3KbTPvYudqX20rqqgcW0fjVIM04xo_LMUOlOcS_qjlIxxjBVmg_y7kDa0MQZXmqfgawgzQ7CZH8IMhzBg3g8x6N1l3z6vXfFpPzY_gD9LANFCVQZorI9fjmGsNZt_kCzci6_c7LuZ5uzw_GN4uqjxsXOvnzUQHo2QTGbm7uLYnNPbySGdjM0VewMeLK5q</recordid><startdate>201008</startdate><enddate>201008</enddate><creator>Enders, J. Tyler</creator><creator>Otto, Thomas J.</creator><creator>Peters, H. Charlie</creator><creator>Wu, Jin</creator><creator>Hardouin, Scott</creator><creator>Moed, Berton R.</creator><creator>Zhang, Zijun</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7QP</scope></search><sort><creationdate>201008</creationdate><title>A model for studying human articular cartilage integration in vitro</title><author>Enders, J. Tyler ; Otto, Thomas J. ; Peters, H. Charlie ; Wu, Jin ; Hardouin, Scott ; Moed, Berton R. ; Zhang, Zijun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4629-110dd52991d8ce081c3e1cb348cb6ab97ac82667fa63c94a8c2dc69af6a18b743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cartilage</topic><topic>Cartilage, Articular - cytology</topic><topic>Cartilage, Articular - physiology</topic><topic>Cells, Cultured</topic><topic>chondrocytes</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - metabolism</topic><topic>Computerized, statistical medical data processing and models in biomedicine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>in vitro</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>integration</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Models and simulation</topic><topic>Models, Biological</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tissue Culture Techniques</topic><topic>tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Enders, J. Tyler</creatorcontrib><creatorcontrib>Otto, Thomas J.</creatorcontrib><creatorcontrib>Peters, H. 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vitro model that can be conveniently applied to simulate and improve the integration of tissue engineered cartilage with native articular cartilage. This model, a cartilage integration construct, consists of a cartilage explant and isolated chondrocytes. The explant was anchored to agarose gel on a culture plate as agarose gelation at 4°C to seal the gap between the bottom of the explant and culture plate surface. Isolated chondrocytes were added and confined in the defect created in the center of the explant. After 4 weeks of culture, neocartilage containing proteoglycans and type II collagen was formed. Minimal integration occurred between the neocartilage and the cartilage explant, resembling the failure of cartilage integration manifested in experimental and clinical cartilage repair. In this model, agarose gel anchors the explant onto culture plate by altering temperatures and effectively prevents “leakage” of the isolated chondrocytes from the defect of the explant. This model provides a convenient simulation of the cartilage integration process in vitro and has applications in studies of cartilage integration and cartilage tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res part A, 2010</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20186769</pmid><doi>10.1002/jbm.a.32719</doi><tpages>6</tpages></addata></record> |
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| subjects | Aged Biological and medical sciences Biotechnology cartilage Cartilage, Articular - cytology Cartilage, Articular - physiology Cells, Cultured chondrocytes Chondrocytes - cytology Chondrocytes - metabolism Computerized, statistical medical data processing and models in biomedicine Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Humans in vitro Industrial applications and implications. Economical aspects integration Medical sciences Middle Aged Miscellaneous Models and simulation Models, Biological Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Tissue Culture Techniques tissue engineering Tissue Engineering - methods |
| title | A model for studying human articular cartilage integration in vitro |
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