First steps towards a Zn/Co(III)sep-driven P450 BM3 reactor

Cytochrome P450s are synthetically attractive hydroxylation catalysts. For cell-free applications, a constant supply of NAD(P)H can be very costly. Mediators such as Zn/Co(III)sep can be an alternative cofactor system to NAD(P)H. Several mutants of cytochrome P450 BM3 with improved electron transfer...

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Veröffentlicht in:	Applied microbiology and biotechnology 2011-08, Vol.91 (4), p.989-999
Hauptverfasser:	Zhao, Liqing, Güven, Güray, Li, Yin, Schwaneberg, Ulrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Amino Acid Substitution - genetics Analysis Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Bioreactors Biotechnological Products and Process Engineering Biotechnology Chloroplasts Coenzymes - metabolism Cytochrome Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Dehydrogenases E coli Electrons Enzymes Enzymes, Immobilized - genetics Enzymes, Immobilized - metabolism Fatty acids Fundamental and applied biological sciences. Psychology Hydrogen peroxide Life Sciences Membrane reactors Methods Microbial Genetics and Genomics Microbiology Mutant Proteins - genetics Mutant Proteins - metabolism NADPH-Ferrihemoprotein Reductase - genetics NADPH-Ferrihemoprotein Reductase - metabolism Phenyl Ethers - metabolism Plug flow Reactors Studies
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