First investigation of spider silk as a braided microsurgical suture
Inhibition of axonal outgrowth accompanied by neuroma formation appears in microsurgical nerve repair as reaction to common microsuture materials like silk, nylon, or polyglycolic acid. In contrast, recent findings revealed advantages of spider silk fibers in guiding Schwann cells in nerve regenerat...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2011-05, Vol.97B (2), p.381-387 |
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| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
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| creator | Kuhbier, Joern W. Reimers, Kerstin Kasper, Cornelia Allmeling, Christina Hillmer, Anja Menger, Björn Vogt, Peter M. Radtke, Christine |
| description | Inhibition of axonal outgrowth accompanied by neuroma formation appears in microsurgical nerve repair as reaction to common microsuture materials like silk, nylon, or polyglycolic acid. In contrast, recent findings revealed advantages of spider silk fibers in guiding Schwann cells in nerve regeneration. Here, we asked if we could braid microsutures from native spider silk fibers. Microsutures braided of native spider dragline silk were manufactured, containing either 2 × 15 or 3 × 10 single fibres strands. Morphologic appearance was studied and tensile strength and stress‐strain ratio (SSR) were calculated. The constructed spider silk sutures showed a median thickness of 25 μm, matching the USP definition of 10‐0. Maximum load and tensile strength for both spider silk microsutures were significantly more than 2‐fold higher than for nylon suture; SSR was 1.5‐fold higher. All values except elasticity were higher in 3 × 10 strand sutures compared to 2 × 15 strand sutures, but not significantly. In this pilot study, we demonstrate the successful manufacture of microsutures from spider silk. With regards to the mechanical properties, these sutures were superior to nylon sutures. As spider silk displays high biocompatibility in nerve regeneration, its usage in microsurgical nerve repair should be considered. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011. |
| doi_str_mv | 10.1002/jbm.b.31825 |
| format | Article |
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In contrast, recent findings revealed advantages of spider silk fibers in guiding Schwann cells in nerve regeneration. Here, we asked if we could braid microsutures from native spider silk fibers. Microsutures braided of native spider dragline silk were manufactured, containing either 2 × 15 or 3 × 10 single fibres strands. Morphologic appearance was studied and tensile strength and stress‐strain ratio (SSR) were calculated. The constructed spider silk sutures showed a median thickness of 25 μm, matching the USP definition of 10‐0. Maximum load and tensile strength for both spider silk microsutures were significantly more than 2‐fold higher than for nylon suture; SSR was 1.5‐fold higher. All values except elasticity were higher in 3 × 10 strand sutures compared to 2 × 15 strand sutures, but not significantly. In this pilot study, we demonstrate the successful manufacture of microsutures from spider silk. With regards to the mechanical properties, these sutures were superior to nylon sutures. As spider silk displays high biocompatibility in nerve regeneration, its usage in microsurgical nerve repair should be considered. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.</description><identifier>ISSN: 1552-4973</identifier><identifier>ISSN: 1552-4981</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.31825</identifier><identifier>PMID: 21432995</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Araneae ; Biocompatible Materials - chemistry ; Biological and medical sciences ; biomaterials ; Braiding ; Elasticity ; Female ; Fibers ; Humans ; Materials Testing ; Medical sciences ; microsurgery ; Microsurgery - instrumentation ; microsuture ; Nephila ; Nerve Regeneration ; Nerves ; Neurosurgery - instrumentation ; Nylons ; Pilot Projects ; Silk ; Silk - chemistry ; spider silk ; Spiders ; Strands ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Sutures ; Technology. Biomaterials. Equipments ; Tensile Strength</subject><ispartof>Journal of biomedical materials research. 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Part B, Applied biomaterials</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Inhibition of axonal outgrowth accompanied by neuroma formation appears in microsurgical nerve repair as reaction to common microsuture materials like silk, nylon, or polyglycolic acid. In contrast, recent findings revealed advantages of spider silk fibers in guiding Schwann cells in nerve regeneration. Here, we asked if we could braid microsutures from native spider silk fibers. Microsutures braided of native spider dragline silk were manufactured, containing either 2 × 15 or 3 × 10 single fibres strands. Morphologic appearance was studied and tensile strength and stress‐strain ratio (SSR) were calculated. The constructed spider silk sutures showed a median thickness of 25 μm, matching the USP definition of 10‐0. Maximum load and tensile strength for both spider silk microsutures were significantly more than 2‐fold higher than for nylon suture; SSR was 1.5‐fold higher. All values except elasticity were higher in 3 × 10 strand sutures compared to 2 × 15 strand sutures, but not significantly. In this pilot study, we demonstrate the successful manufacture of microsutures from spider silk. With regards to the mechanical properties, these sutures were superior to nylon sutures. As spider silk displays high biocompatibility in nerve regeneration, its usage in microsurgical nerve repair should be considered. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.</description><subject>Animals</subject><subject>Araneae</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biological and medical sciences</subject><subject>biomaterials</subject><subject>Braiding</subject><subject>Elasticity</subject><subject>Female</subject><subject>Fibers</subject><subject>Humans</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>microsurgery</subject><subject>Microsurgery - instrumentation</subject><subject>microsuture</subject><subject>Nephila</subject><subject>Nerve Regeneration</subject><subject>Nerves</subject><subject>Neurosurgery - instrumentation</subject><subject>Nylons</subject><subject>Pilot Projects</subject><subject>Silk</subject><subject>Silk - chemistry</subject><subject>spider silk</subject><subject>Spiders</subject><subject>Strands</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. 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| ispartof | Journal of biomedical materials research. Part B, Applied biomaterials, 2011-05, Vol.97B (2), p.381-387 |
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| subjects | Animals Araneae Biocompatible Materials - chemistry Biological and medical sciences biomaterials Braiding Elasticity Female Fibers Humans Materials Testing Medical sciences microsurgery Microsurgery - instrumentation microsuture Nephila Nerve Regeneration Nerves Neurosurgery - instrumentation Nylons Pilot Projects Silk Silk - chemistry spider silk Spiders Strands Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Sutures Technology. Biomaterials. Equipments Tensile Strength |
| title | First investigation of spider silk as a braided microsurgical suture |
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