Polarized hydroxyapatite promotes spread and motility of osteoblastic cells
Osteoblast adhesion to surfaces of implant substrates is recognized as playing a fundamental role in the process of osteoconduction. The purpose of this study was to evaluate the in vitro adhesion of osteoblasts cultured on polarized hydroxyapatite (HA), which provides two kinds of surfaces; negativ...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2010-02, Vol.92A (2), p.783-790 |
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| creator | Nakamura, Miho Nagai, Akiko Tanaka, Yumi Sekijima, Yasutaka Yamashita, Kimihiro |
| description | Osteoblast adhesion to surfaces of implant substrates is recognized as playing a fundamental role in the process of osteoconduction. The purpose of this study was to evaluate the in vitro adhesion of osteoblasts cultured on polarized hydroxyapatite (HA), which provides two kinds of surfaces; negatively charged HA (N‐HA) and positively charged HA (P‐HA). Those surfaces have been proved to enhance the osteobonding capabilities. Osteoblastic cells were seeded onto normal and polarized HA; adhesion and motility of each was observed. Polarization did not affect the percentage of the spread cells against all the adhered cells, but had a significant effect on the spreading of each cell as shown by the measured elongation of the adhered cells by fluorescence observation. The elongation of each cell was especially enhanced on the N‐HA and P‐HA, when compared with normal HA (O‐HA). In addition, the polarization affected cell motility shown by wound healing. Motility analysis showed that the same number of cells started to migrate toward the wound areas on each type of surface. However, the migration of each cell type towards the wound area was accelerated on the N‐HA and P‐HA. The charges induced on the HA surface accelerated the cytoskeleton reorganization of the adhered cells. The acceleration was appeared as cell shape, actin filament pattern such as stress fiber formation, and prolongation of cell motility distance. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010 |
| doi_str_mv | 10.1002/jbm.a.32404 |
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The purpose of this study was to evaluate the in vitro adhesion of osteoblasts cultured on polarized hydroxyapatite (HA), which provides two kinds of surfaces; negatively charged HA (N‐HA) and positively charged HA (P‐HA). Those surfaces have been proved to enhance the osteobonding capabilities. Osteoblastic cells were seeded onto normal and polarized HA; adhesion and motility of each was observed. Polarization did not affect the percentage of the spread cells against all the adhered cells, but had a significant effect on the spreading of each cell as shown by the measured elongation of the adhered cells by fluorescence observation. The elongation of each cell was especially enhanced on the N‐HA and P‐HA, when compared with normal HA (O‐HA). In addition, the polarization affected cell motility shown by wound healing. Motility analysis showed that the same number of cells started to migrate toward the wound areas on each type of surface. However, the migration of each cell type towards the wound area was accelerated on the N‐HA and P‐HA. The charges induced on the HA surface accelerated the cytoskeleton reorganization of the adhered cells. The acceleration was appeared as cell shape, actin filament pattern such as stress fiber formation, and prolongation of cell motility distance. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010</description><identifier>ISSN: 1549-3296</identifier><identifier>ISSN: 1552-4965</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.32404</identifier><identifier>PMID: 19274714</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>3T3 Cells ; Animals ; Biocompatible Materials - pharmacology ; Biological and medical sciences ; Bone Regeneration - drug effects ; cell adhesion ; Cell Adhesion - drug effects ; cell motility ; Cell Movement - drug effects ; Cell Proliferation - drug effects ; Cytoskeleton - drug effects ; Cytoskeleton - ultrastructure ; Durapatite - pharmacology ; Fluorescent Dyes ; hydroxyapatite ; Medical sciences ; Mice ; Orthopedic surgery ; osteoblast ; Osteoblasts - drug effects ; osteoconduction ; Surface Properties ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Osteoblast adhesion to surfaces of implant substrates is recognized as playing a fundamental role in the process of osteoconduction. The purpose of this study was to evaluate the in vitro adhesion of osteoblasts cultured on polarized hydroxyapatite (HA), which provides two kinds of surfaces; negatively charged HA (N‐HA) and positively charged HA (P‐HA). Those surfaces have been proved to enhance the osteobonding capabilities. Osteoblastic cells were seeded onto normal and polarized HA; adhesion and motility of each was observed. Polarization did not affect the percentage of the spread cells against all the adhered cells, but had a significant effect on the spreading of each cell as shown by the measured elongation of the adhered cells by fluorescence observation. The elongation of each cell was especially enhanced on the N‐HA and P‐HA, when compared with normal HA (O‐HA). In addition, the polarization affected cell motility shown by wound healing. Motility analysis showed that the same number of cells started to migrate toward the wound areas on each type of surface. However, the migration of each cell type towards the wound area was accelerated on the N‐HA and P‐HA. The charges induced on the HA surface accelerated the cytoskeleton reorganization of the adhered cells. The acceleration was appeared as cell shape, actin filament pattern such as stress fiber formation, and prolongation of cell motility distance. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Bone Regeneration - drug effects</subject><subject>cell adhesion</subject><subject>Cell Adhesion - drug effects</subject><subject>cell motility</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton - ultrastructure</subject><subject>Durapatite - pharmacology</subject><subject>Fluorescent Dyes</subject><subject>hydroxyapatite</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Orthopedic surgery</subject><subject>osteoblast</subject><subject>Osteoblasts - drug effects</subject><subject>osteoconduction</subject><subject>Surface Properties</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><issn>1549-3296</issn><issn>1552-4965</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUha0K1Besuq-yQSyqDH47XpYKyqMtLEZiad3Yjuo2GQ92RjT8ehxmaHd0Zcv67jnn-iB0QvCCYEzf3bXDAhaMcsz30CERgtZcS_FivnNdM6rlATrK-a7AEgu6jw6Ipoorwg_R1--xhxR-e1fdTi7FhwnWMIbRV-sUhzj6XOV18uAqWLmqPIQ-jFMVuyrm0ce2hzwGW1nf9_kVetlBn_3r3XmMlh8_LC8-1VffLj9fnF_VVtCG1520Hrq2bbB0nljhPHUMLLONUE6DpUQT0EKyRsmyAJdaMFuyc6GgUYwdo7db2ZLw58bn0QwhzwFg5eMmm6ZhmGKO5fNkkVYKU_UsWWxVM_9qIc-2pE0x5-Q7s05hgDQZgs3chyl9GDB_-yj06U530w7ePbG7AgrwZgdAttB3CVY25EeOFhXBybwK2XK_Qu-n_3maL--v_5nX25lQqnp4nIF0b6RiSpgfN5fmZqmVEOzaMPYH5eyw-A</recordid><startdate>201002</startdate><enddate>201002</enddate><creator>Nakamura, Miho</creator><creator>Nagai, Akiko</creator><creator>Tanaka, Yumi</creator><creator>Sekijima, Yasutaka</creator><creator>Yamashita, Kimihiro</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7QP</scope></search><sort><creationdate>201002</creationdate><title>Polarized hydroxyapatite promotes spread and motility of osteoblastic cells</title><author>Nakamura, Miho ; Nagai, Akiko ; Tanaka, Yumi ; Sekijima, Yasutaka ; Yamashita, Kimihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5284-f6ceafbb806de1c5de2d3ac3c857d9ac2191a956387654946953c002457a8733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Bone Regeneration - drug effects</topic><topic>cell adhesion</topic><topic>Cell Adhesion - drug effects</topic><topic>cell motility</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cytoskeleton - drug effects</topic><topic>Cytoskeleton - ultrastructure</topic><topic>Durapatite - pharmacology</topic><topic>Fluorescent Dyes</topic><topic>hydroxyapatite</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Orthopedic surgery</topic><topic>osteoblast</topic><topic>Osteoblasts - drug effects</topic><topic>osteoconduction</topic><topic>Surface Properties</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. 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The purpose of this study was to evaluate the in vitro adhesion of osteoblasts cultured on polarized hydroxyapatite (HA), which provides two kinds of surfaces; negatively charged HA (N‐HA) and positively charged HA (P‐HA). Those surfaces have been proved to enhance the osteobonding capabilities. Osteoblastic cells were seeded onto normal and polarized HA; adhesion and motility of each was observed. Polarization did not affect the percentage of the spread cells against all the adhered cells, but had a significant effect on the spreading of each cell as shown by the measured elongation of the adhered cells by fluorescence observation. The elongation of each cell was especially enhanced on the N‐HA and P‐HA, when compared with normal HA (O‐HA). In addition, the polarization affected cell motility shown by wound healing. Motility analysis showed that the same number of cells started to migrate toward the wound areas on each type of surface. However, the migration of each cell type towards the wound area was accelerated on the N‐HA and P‐HA. The charges induced on the HA surface accelerated the cytoskeleton reorganization of the adhered cells. The acceleration was appeared as cell shape, actin filament pattern such as stress fiber formation, and prolongation of cell motility distance. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19274714</pmid><doi>10.1002/jbm.a.32404</doi><tpages>8</tpages></addata></record> |
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| subjects | 3T3 Cells Animals Biocompatible Materials - pharmacology Biological and medical sciences Bone Regeneration - drug effects cell adhesion Cell Adhesion - drug effects cell motility Cell Movement - drug effects Cell Proliferation - drug effects Cytoskeleton - drug effects Cytoskeleton - ultrastructure Durapatite - pharmacology Fluorescent Dyes hydroxyapatite Medical sciences Mice Orthopedic surgery osteoblast Osteoblasts - drug effects osteoconduction Surface Properties Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments |
| title | Polarized hydroxyapatite promotes spread and motility of osteoblastic cells |
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