Neuroprotective effect of mesna (2-mercaptoethane sulfonate) against spinal cord ischemia/reperfusion injury in rabbits
Abstract Although the precise mechanism by which ischemia/reperfusion injury occurs in the spinal cord remains unclear, it is evident that free oxygen radicals and apoptosis play major roles in the destruction of membrane lipids, damage to DNA and cell death. The apoptotic process involves activatio...
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| Veröffentlicht in: | Journal of clinical neuroscience 2010-04, Vol.17 (4), p.486-489 |
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| creator | Dolgun, Habibullah Sekerci, Zeki Turkoglu, Erhan Kertmen, Hayri Yilmaz, Erdal R Anlar, Murat Erguder, Imge B Tuna, Hakan |
| description | Abstract Although the precise mechanism by which ischemia/reperfusion injury occurs in the spinal cord remains unclear, it is evident that free oxygen radicals and apoptosis play major roles in the destruction of membrane lipids, damage to DNA and cell death. The apoptotic process involves activation of the caspase-3 cascade. Although it is widely used as a protective agent against cell injury, it is unknown whether mesna (2-mercaptoethane sulfonate) ameliorates neuronal ischemic injury. The aim of this study was to determine the effect of mesna on caspase-3 activity in a rabbit model. Adult rabbits underwent spinal cord ischemic injury via occlusion of the abdominal aorta for 20 min. Twenty-four hours after ischemia, spinal cord samples were obtained and tissue caspase-3 activity was measured. Rabbits that had been given a single dose of 150 mg/kg mesna had decreased caspase-3 activity in the spinal cord following ischemia/reperfusion injury, indicating a protective effect. However, caspase-3 activity was lower in rabbits given methylprednisolone than in those given mesna, indicating that methylprednisolone has the stronger protective effect of the two agents. |
| doi_str_mv | 10.1016/j.jocn.2009.07.108 |
| format | Article |
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The apoptotic process involves activation of the caspase-3 cascade. Although it is widely used as a protective agent against cell injury, it is unknown whether mesna (2-mercaptoethane sulfonate) ameliorates neuronal ischemic injury. The aim of this study was to determine the effect of mesna on caspase-3 activity in a rabbit model. Adult rabbits underwent spinal cord ischemic injury via occlusion of the abdominal aorta for 20 min. Twenty-four hours after ischemia, spinal cord samples were obtained and tissue caspase-3 activity was measured. Rabbits that had been given a single dose of 150 mg/kg mesna had decreased caspase-3 activity in the spinal cord following ischemia/reperfusion injury, indicating a protective effect. However, caspase-3 activity was lower in rabbits given methylprednisolone than in those given mesna, indicating that methylprednisolone has the stronger protective effect of the two agents.</description><identifier>ISSN: 0967-5868</identifier><identifier>EISSN: 1532-2653</identifier><identifier>DOI: 10.1016/j.jocn.2009.07.108</identifier><identifier>PMID: 20117935</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>2-Mercaptoethane sulfonate ; Animals ; Apoptosis ; Apoptosis - drug effects ; Caspase ; Caspase 3 - drug effects ; Female ; Ischemia/reperfusion injury ; Mesna ; Mesna - pharmacology ; Methylprednisolone - pharmacology ; Neurology ; Neuroprotective Agents - pharmacology ; Rabbits ; Reperfusion Injury - prevention & control ; Spinal Cord Injuries - prevention & control ; Spinal Cord Ischemia - prevention & control</subject><ispartof>Journal of clinical neuroscience, 2010-04, Vol.17 (4), p.486-489</ispartof><rights>2009</rights><rights>(c) 2009 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-bfe2fe03471f14461d93b21ba707a43e4cc5252b24464e6800ee5c1dbda20c6f3</citedby><cites>FETCH-LOGICAL-c442t-bfe2fe03471f14461d93b21ba707a43e4cc5252b24464e6800ee5c1dbda20c6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jocn.2009.07.108$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20117935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dolgun, Habibullah</creatorcontrib><creatorcontrib>Sekerci, Zeki</creatorcontrib><creatorcontrib>Turkoglu, Erhan</creatorcontrib><creatorcontrib>Kertmen, Hayri</creatorcontrib><creatorcontrib>Yilmaz, Erdal R</creatorcontrib><creatorcontrib>Anlar, Murat</creatorcontrib><creatorcontrib>Erguder, Imge B</creatorcontrib><creatorcontrib>Tuna, Hakan</creatorcontrib><title>Neuroprotective effect of mesna (2-mercaptoethane sulfonate) against spinal cord ischemia/reperfusion injury in rabbits</title><title>Journal of clinical neuroscience</title><addtitle>J Clin Neurosci</addtitle><description>Abstract Although the precise mechanism by which ischemia/reperfusion injury occurs in the spinal cord remains unclear, it is evident that free oxygen radicals and apoptosis play major roles in the destruction of membrane lipids, damage to DNA and cell death. The apoptotic process involves activation of the caspase-3 cascade. Although it is widely used as a protective agent against cell injury, it is unknown whether mesna (2-mercaptoethane sulfonate) ameliorates neuronal ischemic injury. The aim of this study was to determine the effect of mesna on caspase-3 activity in a rabbit model. Adult rabbits underwent spinal cord ischemic injury via occlusion of the abdominal aorta for 20 min. Twenty-four hours after ischemia, spinal cord samples were obtained and tissue caspase-3 activity was measured. Rabbits that had been given a single dose of 150 mg/kg mesna had decreased caspase-3 activity in the spinal cord following ischemia/reperfusion injury, indicating a protective effect. However, caspase-3 activity was lower in rabbits given methylprednisolone than in those given mesna, indicating that methylprednisolone has the stronger protective effect of the two agents.</description><subject>2-Mercaptoethane sulfonate</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Caspase</subject><subject>Caspase 3 - drug effects</subject><subject>Female</subject><subject>Ischemia/reperfusion injury</subject><subject>Mesna</subject><subject>Mesna - pharmacology</subject><subject>Methylprednisolone - pharmacology</subject><subject>Neurology</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Rabbits</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Spinal Cord Injuries - prevention & control</subject><subject>Spinal Cord Ischemia - prevention & control</subject><issn>0967-5868</issn><issn>1532-2653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstu1TAQhi0EoofCC7BA3gGLnI4vuUmoEqq4VKrKAlhbjjOmDokd7KTovH0dncKCRVnNaPzPL898Q8hLBnsGrDob9kMwfs8B2j3UudY8IjtWCl7wqhSPyQ7aqi7KpmpOyLOUBshCKeApOeHAWN2Kckd-X-MawxzDgmZxt0jR2pzRYOmEyWv6hhcTRqPnJeByoz3StI42eL3gW6p_aOfTQtPsvB6pCbGnLpkbnJw-izhjtGtywVPnhzUecqBRd51b0nPyxOox4Yv7eEq-f_zw7eJzcfXl0-XF-6vCSMmXorPILYKQNbNMyor1reg463QNtZYCpTElL3nH85vEqgFALA3ru15zMJUVp-T10TeP-GvFtKgpfxDHMU8S1qSaRgBrG9n8V1kLwVvOSp6V_Kg0MaQU0ao5uknHg2KgNjJqUBsZtZFRUOfaZv_q3n7tJuz_tvxBkQXvjgLM67h1GFUyDr3B3sVMRPXBPex__k-7GZ13Ro8_8YBpCGvMiJJiKnEF6ut2G9tpQAtQsbYUd0qbtiQ</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Dolgun, Habibullah</creator><creator>Sekerci, Zeki</creator><creator>Turkoglu, Erhan</creator><creator>Kertmen, Hayri</creator><creator>Yilmaz, Erdal R</creator><creator>Anlar, Murat</creator><creator>Erguder, Imge B</creator><creator>Tuna, Hakan</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20100401</creationdate><title>Neuroprotective effect of mesna (2-mercaptoethane sulfonate) against spinal cord ischemia/reperfusion injury in rabbits</title><author>Dolgun, Habibullah ; 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The apoptotic process involves activation of the caspase-3 cascade. Although it is widely used as a protective agent against cell injury, it is unknown whether mesna (2-mercaptoethane sulfonate) ameliorates neuronal ischemic injury. The aim of this study was to determine the effect of mesna on caspase-3 activity in a rabbit model. Adult rabbits underwent spinal cord ischemic injury via occlusion of the abdominal aorta for 20 min. Twenty-four hours after ischemia, spinal cord samples were obtained and tissue caspase-3 activity was measured. Rabbits that had been given a single dose of 150 mg/kg mesna had decreased caspase-3 activity in the spinal cord following ischemia/reperfusion injury, indicating a protective effect. 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| subjects | 2-Mercaptoethane sulfonate Animals Apoptosis Apoptosis - drug effects Caspase Caspase 3 - drug effects Female Ischemia/reperfusion injury Mesna Mesna - pharmacology Methylprednisolone - pharmacology Neurology Neuroprotective Agents - pharmacology Rabbits Reperfusion Injury - prevention & control Spinal Cord Injuries - prevention & control Spinal Cord Ischemia - prevention & control |
| title | Neuroprotective effect of mesna (2-mercaptoethane sulfonate) against spinal cord ischemia/reperfusion injury in rabbits |
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