Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells
Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic...
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Veröffentlicht in: | Molecular immunology 2008-09, Vol.45 (15), p.3965-3973 |
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container_title | Molecular immunology |
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creator | Papadimitriou, Lina Morianos, Ioannis Michailidou, Valentina Dionyssopoulou, Eva Vassiliadis, Simon Athanassakis, Irene |
description | Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic cells were examined as to intracellular HLA-DR, -DM and -DO expression, if any. Immunofluorescence scored by UV-microscopy, flow cytometry or confocal microscope analysis detected intracellular pools of HLA-DR, -DO and to a lesser degree HLA-DM, whereas sub-cellular fractionation localized these molecules within endosomes. RT-PCR experiments revealed the presence of HLA-DRαβ, HLA-DMαβ and HLA-DOβ but not HLA-DOα transcripts. Despite the absence of the HLA-DOα chain, stable transfectants of K562 with a full length HLA-DOβ-EGFP construct showed that DOβ chain could be translocated to endosomes and form stable complexes with HLA-DR. Such complexes could be responsible for arresting HLA-DR molecules within endosomes, maintaining their surface class-II negative state. |
doi_str_mv | 10.1016/j.molimm.2008.06.017 |
format | Article |
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The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic cells were examined as to intracellular HLA-DR, -DM and -DO expression, if any. Immunofluorescence scored by UV-microscopy, flow cytometry or confocal microscope analysis detected intracellular pools of HLA-DR, -DO and to a lesser degree HLA-DM, whereas sub-cellular fractionation localized these molecules within endosomes. RT-PCR experiments revealed the presence of HLA-DRαβ, HLA-DMαβ and HLA-DOβ but not HLA-DOα transcripts. Despite the absence of the HLA-DOα chain, stable transfectants of K562 with a full length HLA-DOβ-EGFP construct showed that DOβ chain could be translocated to endosomes and form stable complexes with HLA-DR. Such complexes could be responsible for arresting HLA-DR molecules within endosomes, maintaining their surface class-II negative state.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/j.molimm.2008.06.017</identifier><identifier>PMID: 18657863</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Endosomes - metabolism ; HL-60 Cells ; HL-60/K562 cells ; HLA-D Antigens - metabolism ; HLA-DM ; HLA-DO ; HLA-DR ; HLA-DR Antigens - metabolism ; Humans ; K562 Cells ; Leukemia, Myeloid ; Reverse Transcriptase Polymerase Chain Reaction</subject><ispartof>Molecular immunology, 2008-09, Vol.45 (15), p.3965-3973</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-4b56dd92a42b26bca7d4923552134d8eae8c010f67884d05a1ba7b5864e8c0c93</citedby><cites>FETCH-LOGICAL-c423t-4b56dd92a42b26bca7d4923552134d8eae8c010f67884d05a1ba7b5864e8c0c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.molimm.2008.06.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18657863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Papadimitriou, Lina</creatorcontrib><creatorcontrib>Morianos, Ioannis</creatorcontrib><creatorcontrib>Michailidou, Valentina</creatorcontrib><creatorcontrib>Dionyssopoulou, Eva</creatorcontrib><creatorcontrib>Vassiliadis, Simon</creatorcontrib><creatorcontrib>Athanassakis, Irene</creatorcontrib><title>Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic cells were examined as to intracellular HLA-DR, -DM and -DO expression, if any. Immunofluorescence scored by UV-microscopy, flow cytometry or confocal microscope analysis detected intracellular pools of HLA-DR, -DO and to a lesser degree HLA-DM, whereas sub-cellular fractionation localized these molecules within endosomes. RT-PCR experiments revealed the presence of HLA-DRαβ, HLA-DMαβ and HLA-DOβ but not HLA-DOα transcripts. Despite the absence of the HLA-DOα chain, stable transfectants of K562 with a full length HLA-DOβ-EGFP construct showed that DOβ chain could be translocated to endosomes and form stable complexes with HLA-DR. Such complexes could be responsible for arresting HLA-DR molecules within endosomes, maintaining their surface class-II negative state.</description><subject>Endosomes - metabolism</subject><subject>HL-60 Cells</subject><subject>HL-60/K562 cells</subject><subject>HLA-D Antigens - metabolism</subject><subject>HLA-DM</subject><subject>HLA-DO</subject><subject>HLA-DR</subject><subject>HLA-DR Antigens - metabolism</subject><subject>Humans</subject><subject>K562 Cells</subject><subject>Leukemia, Myeloid</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7uzqPxDJSS_bbSWdpNMXYZlRRxxZEL0JIZ1UY8b-WJNuQX-9aWfA23oKVJ63qqiHkGcMSgZMvTqWw9SHYSg5gC5BlcDqB2TDdM2Lhgn-kGwyxgqpG7gglykdAUCBko_JBdNK1lpVG_J1-81G62aM4bedwzTSqaNhnHMN-37pbaT7w02x-3RNdx-pHT3d3dK7OHWhx8zRD1Lxv-X9oVBAe1y-4xAcXdPpCXnU2T7h0_N7Rb68ffN5uy8Ot-_eb28OhRO8mgvRSuV9w63gLVets7UXDa-k5KwSXqNF7YBBp2qthQdpWWvrVmol1g_XVFfk5alvXuzHgmk2Q0jrBnbEaUlG6yqfomIr-eJeUjVCMQH8vyCHpq4F1xkUJ9DFKaWInbmLYbDxl2FgVlHmaE6izCrKgDJZVI49P_df2gH9v9DZTAZenwDMh_sZMJrkAo4OfYjoZuOncP-EPylTor0</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Papadimitriou, Lina</creator><creator>Morianos, Ioannis</creator><creator>Michailidou, Valentina</creator><creator>Dionyssopoulou, Eva</creator><creator>Vassiliadis, Simon</creator><creator>Athanassakis, Irene</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells</title><author>Papadimitriou, Lina ; Morianos, Ioannis ; Michailidou, Valentina ; Dionyssopoulou, Eva ; Vassiliadis, Simon ; Athanassakis, Irene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-4b56dd92a42b26bca7d4923552134d8eae8c010f67884d05a1ba7b5864e8c0c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Endosomes - metabolism</topic><topic>HL-60 Cells</topic><topic>HL-60/K562 cells</topic><topic>HLA-D Antigens - metabolism</topic><topic>HLA-DM</topic><topic>HLA-DO</topic><topic>HLA-DR</topic><topic>HLA-DR Antigens - metabolism</topic><topic>Humans</topic><topic>K562 Cells</topic><topic>Leukemia, Myeloid</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Papadimitriou, Lina</creatorcontrib><creatorcontrib>Morianos, Ioannis</creatorcontrib><creatorcontrib>Michailidou, Valentina</creatorcontrib><creatorcontrib>Dionyssopoulou, Eva</creatorcontrib><creatorcontrib>Vassiliadis, Simon</creatorcontrib><creatorcontrib>Athanassakis, Irene</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Papadimitriou, Lina</au><au>Morianos, Ioannis</au><au>Michailidou, Valentina</au><au>Dionyssopoulou, Eva</au><au>Vassiliadis, Simon</au><au>Athanassakis, Irene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>45</volume><issue>15</issue><spage>3965</spage><epage>3973</epage><pages>3965-3973</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><abstract>Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic cells were examined as to intracellular HLA-DR, -DM and -DO expression, if any. Immunofluorescence scored by UV-microscopy, flow cytometry or confocal microscope analysis detected intracellular pools of HLA-DR, -DO and to a lesser degree HLA-DM, whereas sub-cellular fractionation localized these molecules within endosomes. RT-PCR experiments revealed the presence of HLA-DRαβ, HLA-DMαβ and HLA-DOβ but not HLA-DOα transcripts. Despite the absence of the HLA-DOα chain, stable transfectants of K562 with a full length HLA-DOβ-EGFP construct showed that DOβ chain could be translocated to endosomes and form stable complexes with HLA-DR. Such complexes could be responsible for arresting HLA-DR molecules within endosomes, maintaining their surface class-II negative state.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>18657863</pmid><doi>10.1016/j.molimm.2008.06.017</doi><tpages>9</tpages></addata></record> |
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subjects | Endosomes - metabolism HL-60 Cells HL-60/K562 cells HLA-D Antigens - metabolism HLA-DM HLA-DO HLA-DR HLA-DR Antigens - metabolism Humans K562 Cells Leukemia, Myeloid Reverse Transcriptase Polymerase Chain Reaction |
title | Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells |
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