Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells

Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic...

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Veröffentlicht in:Molecular immunology 2008-09, Vol.45 (15), p.3965-3973
Hauptverfasser: Papadimitriou, Lina, Morianos, Ioannis, Michailidou, Valentina, Dionyssopoulou, Eva, Vassiliadis, Simon, Athanassakis, Irene
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container_end_page 3973
container_issue 15
container_start_page 3965
container_title Molecular immunology
container_volume 45
creator Papadimitriou, Lina
Morianos, Ioannis
Michailidou, Valentina
Dionyssopoulou, Eva
Vassiliadis, Simon
Athanassakis, Irene
description Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic cells were examined as to intracellular HLA-DR, -DM and -DO expression, if any. Immunofluorescence scored by UV-microscopy, flow cytometry or confocal microscope analysis detected intracellular pools of HLA-DR, -DO and to a lesser degree HLA-DM, whereas sub-cellular fractionation localized these molecules within endosomes. RT-PCR experiments revealed the presence of HLA-DRαβ, HLA-DMαβ and HLA-DOβ but not HLA-DOα transcripts. Despite the absence of the HLA-DOα chain, stable transfectants of K562 with a full length HLA-DOβ-EGFP construct showed that DOβ chain could be translocated to endosomes and form stable complexes with HLA-DR. Such complexes could be responsible for arresting HLA-DR molecules within endosomes, maintaining their surface class-II negative state.
doi_str_mv 10.1016/j.molimm.2008.06.017
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subjects Endosomes - metabolism
HL-60 Cells
HL-60/K562 cells
HLA-D Antigens - metabolism
HLA-DM
HLA-DO
HLA-DR
HLA-DR Antigens - metabolism
Humans
K562 Cells
Leukemia, Myeloid
Reverse Transcriptase Polymerase Chain Reaction
title Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells
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