Identification of Semiochemicals Released by Cotton, Gossypium hirsutum, Upon Infestation by the Cotton Aphid, Aphis gossypii

The cotton aphid, Aphis gossypii (Homoptera: Aphididae), is increasing in importance as a pest worldwide since the introduction of Bt-cotton, which controls lepidopteran but not homopteran pests. The chemical ecology of interactions between cotton, Gossypium hirsutum (Malvaceae), A. gossypii , and t...

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Veröffentlicht in:Journal of chemical ecology 2011-07, Vol.37 (7), p.741-750
Hauptverfasser: Hegde, Mahabaleshwar, Oliveira, Janser N., da Costa, Joao G., Bleicher, Ervino, Santana, Antonio E. G., Bruce, Toby J. A., Caulfield, John, Dewhirst, Sarah Y., Woodcock, Christine M., Pickett, John A., Birkett, Michael A.
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container_end_page 750
container_issue 7
container_start_page 741
container_title Journal of chemical ecology
container_volume 37
creator Hegde, Mahabaleshwar
Oliveira, Janser N.
da Costa, Joao G.
Bleicher, Ervino
Santana, Antonio E. G.
Bruce, Toby J. A.
Caulfield, John
Dewhirst, Sarah Y.
Woodcock, Christine M.
Pickett, John A.
Birkett, Michael A.
description The cotton aphid, Aphis gossypii (Homoptera: Aphididae), is increasing in importance as a pest worldwide since the introduction of Bt-cotton, which controls lepidopteran but not homopteran pests. The chemical ecology of interactions between cotton, Gossypium hirsutum (Malvaceae), A. gossypii , and the predatory lacewing Chrysoperla lucasina (Neuroptera: Chrysopidae), was investigated with a view to providing new pest management strategies. Behavioral tests using a four-arm (Pettersson) olfactometer showed that alate A. gossypii spent significantly more time in the presence of odor from uninfested cotton seedlings compared to clean air, but significantly less time in the presence of odor from A. gossypii infested plants. A. gossypii also spent significantly more time in the presence of headspace samples of volatile organic compounds (VOCs) obtained from uninfested cotton seedlings, but significantly less time with those from A. gossypii infested plants. VOCs from uninfested and A. gossypii infested cotton seedlings were analyzed by gas chromatography (GC) and coupled GC-mass spectrometry (GC-MS), leading to the identification of ( Z )-3-hexenyl acetate, ( E )-4,8-dimethyl-1,3,7-nonatriene (DMNT), methyl salicylate, and ( E,E )-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), which were produced in larger amounts from A. gossypii infested plants compared to uninfested plants. In behavioral tests, A. gossypii spent significantly more time in the control (solvent) arms when presented with a synthetic blend of these four compounds, with and without the presence of VOCs from uninfested cotton. Coupled GC-electroantennogram (EAG) recordings with the lacewing C. lucasina showed significant antennal responses to VOCs from A. gossypii infested cotton, suggesting they have a role in indirect defense and indicating a likely behavioral role for these compounds for the predator as well as the aphid.
doi_str_mv 10.1007/s10886-011-9980-x
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The chemical ecology of interactions between cotton, Gossypium hirsutum (Malvaceae), A. gossypii , and the predatory lacewing Chrysoperla lucasina (Neuroptera: Chrysopidae), was investigated with a view to providing new pest management strategies. Behavioral tests using a four-arm (Pettersson) olfactometer showed that alate A. gossypii spent significantly more time in the presence of odor from uninfested cotton seedlings compared to clean air, but significantly less time in the presence of odor from A. gossypii infested plants. A. gossypii also spent significantly more time in the presence of headspace samples of volatile organic compounds (VOCs) obtained from uninfested cotton seedlings, but significantly less time with those from A. gossypii infested plants. VOCs from uninfested and A. gossypii infested cotton seedlings were analyzed by gas chromatography (GC) and coupled GC-mass spectrometry (GC-MS), leading to the identification of ( Z )-3-hexenyl acetate, ( E )-4,8-dimethyl-1,3,7-nonatriene (DMNT), methyl salicylate, and ( E,E )-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), which were produced in larger amounts from A. gossypii infested plants compared to uninfested plants. In behavioral tests, A. gossypii spent significantly more time in the control (solvent) arms when presented with a synthetic blend of these four compounds, with and without the presence of VOCs from uninfested cotton. Coupled GC-electroantennogram (EAG) recordings with the lacewing C. lucasina showed significant antennal responses to VOCs from A. gossypii infested cotton, suggesting they have a role in indirect defense and indicating a likely behavioral role for these compounds for the predator as well as the aphid.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>21671083</pmid><doi>10.1007/s10886-011-9980-x</doi><tpages>10</tpages></addata></record>
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subjects Acetates - isolation & purification
Acetic acid
Agriculture
Alkenes - isolation & purification
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Aphididae
Aphids - physiology
Aquatic insects
Behavior, Animal - physiology
Biochemistry
Biological and medical sciences
Biological Microscopy
Biomedical and Life Sciences
Chemical ecology
Cotton
Ecology
Entomology
Fundamental and applied biological sciences. Psychology
Gas chromatography
Gas Chromatography-Mass Spectrometry
General aspects
Gossypium - chemistry
Gossypium - physiology
Insects
Life Sciences
Mass spectrometry
Odors
Organic compounds
Pest control
Pests
Pheromones - isolation & purification
Seedlings
Terpenes - isolation & purification
VOCs
Volatile organic compounds
Volatile Organic Compounds - isolation & purification
title Identification of Semiochemicals Released by Cotton, Gossypium hirsutum, Upon Infestation by the Cotton Aphid, Aphis gossypii
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