Improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/ isooctane reverse micellar system
The activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-l-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (PEG 400) is added...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2003-08, Vol.110 (2), p.101-112 |
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| creator | TALUKDER, M. M. R TAKEYAMA, T HAYASHI, Y WU, J. C KAWANISHI, T SHIMIZU, N OGINO, C |
| description | The activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-l-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (PEG 400) is added to the reverse micelles. To understand the effect of PEG 400 on the phase behavior of the reverse micellar system, the phase diagram of AOT/ PEG 400/water/isooctane system was studied. The influences of relevant parameters on the catalytic activity in AOT/PEG 400 reverse micelles were investigated and compared with the results in the simple AOT reverse micelles. In the presence of PEG 400, the linear decreasing trend of the lipase activity with AOT concentration, which is observed in the simple AOT reverse micelles, disappeared. Enzyme entrapped in AOT/PEG reverse micelles was very stable, retaining >75% of its initial activity after 60 d, whereas the half-life in simple AOT reverse micelles was 38 d. The kinetics parameter maximum velocity (Vmax) exhibiting the temperature dependence and the activation energy obtained by Arrhenius plot was suppressed significantly by the addition of PEG 400. |
| doi_str_mv | 10.1385/ABAB:110:2:101 |
| format | Article |
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M. R ; TAKEYAMA, T ; HAYASHI, Y ; WU, J. C ; KAWANISHI, T ; SHIMIZU, N ; OGINO, C</creator><creatorcontrib>TALUKDER, M. M. R ; TAKEYAMA, T ; HAYASHI, Y ; WU, J. C ; KAWANISHI, T ; SHIMIZU, N ; OGINO, C</creatorcontrib><description>The activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-l-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (PEG 400) is added to the reverse micelles. To understand the effect of PEG 400 on the phase behavior of the reverse micellar system, the phase diagram of AOT/ PEG 400/water/isooctane system was studied. The influences of relevant parameters on the catalytic activity in AOT/PEG 400 reverse micelles were investigated and compared with the results in the simple AOT reverse micelles. In the presence of PEG 400, the linear decreasing trend of the lipase activity with AOT concentration, which is observed in the simple AOT reverse micelles, disappeared. Enzyme entrapped in AOT/PEG reverse micelles was very stable, retaining >75% of its initial activity after 60 d, whereas the half-life in simple AOT reverse micelles was 38 d. The kinetics parameter maximum velocity (Vmax) exhibiting the temperature dependence and the activation energy obtained by Arrhenius plot was suppressed significantly by the addition of PEG 400.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>EISSN: 0273-2289</identifier><identifier>DOI: 10.1385/ABAB:110:2:101</identifier><identifier>PMID: 14515025</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; Biotechnology ; Chromobacterium - enzymology ; Chromobacterium viscosum ; Dioctyl Sulfosuccinic Acid - metabolism ; Enzymatic activity ; Enzyme Activation ; Enzyme Stability ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Lipase - metabolism ; Micelles ; Molecular Weight ; Octanes - chemistry ; Olea ; Olive oil ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; Polyethylene Glycols - pharmacology ; Sodium ; Structure-Activity Relationship ; Studies</subject><ispartof>Applied biochemistry and biotechnology, 2003-08, Vol.110 (2), p.101-112</ispartof><rights>2004 INIST-CNRS</rights><rights>Humana Press Inc. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-11816919bb83b249f89967669e180d5b4a1e0380550f5f7bec9ffb7faa3958a83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15120447$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14515025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TALUKDER, M. M. R</creatorcontrib><creatorcontrib>TAKEYAMA, T</creatorcontrib><creatorcontrib>HAYASHI, Y</creatorcontrib><creatorcontrib>WU, J. C</creatorcontrib><creatorcontrib>KAWANISHI, T</creatorcontrib><creatorcontrib>SHIMIZU, N</creatorcontrib><creatorcontrib>OGINO, C</creatorcontrib><title>Improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/ isooctane reverse micellar system</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><description>The activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-l-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (PEG 400) is added to the reverse micelles. To understand the effect of PEG 400 on the phase behavior of the reverse micellar system, the phase diagram of AOT/ PEG 400/water/isooctane system was studied. The influences of relevant parameters on the catalytic activity in AOT/PEG 400 reverse micelles were investigated and compared with the results in the simple AOT reverse micelles. In the presence of PEG 400, the linear decreasing trend of the lipase activity with AOT concentration, which is observed in the simple AOT reverse micelles, disappeared. Enzyme entrapped in AOT/PEG reverse micelles was very stable, retaining >75% of its initial activity after 60 d, whereas the half-life in simple AOT reverse micelles was 38 d. The kinetics parameter maximum velocity (Vmax) exhibiting the temperature dependence and the activation energy obtained by Arrhenius plot was suppressed significantly by the addition of PEG 400.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromobacterium - enzymology</subject><subject>Chromobacterium viscosum</subject><subject>Dioctyl Sulfosuccinic Acid - metabolism</subject><subject>Enzymatic activity</subject><subject>Enzyme Activation</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lipase - metabolism</subject><subject>Micelles</subject><subject>Molecular Weight</subject><subject>Octanes - chemistry</subject><subject>Olea</subject><subject>Olive oil</subject><subject>Polyethylene glycol</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Sodium</subject><subject>Structure-Activity Relationship</subject><subject>Studies</subject><issn>0273-2289</issn><issn>1559-0291</issn><issn>0273-2289</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0ktv1DAQAOAIgehSuHJEFhKvQ1qPEyf23rYVj0orcYFz5DjjrisnXmJnS_hd_ECcdqVKHOBkefzNjG1Nlr0EegaF4Oebi83FGoCu2RooPMpWwLnMKZPwOFtRVhc5Y0KeZM9CuKEUmOD10-wESg6cMr7Kfl_1-9EfsMchEjsQHH7NPRKloz3YOBM1dCRE1Vq37NoU6DobrR-IN8T5W9J7h3pyaiS3aK93key9mzHuZocDkms3a-9I9CT4zk49aW14z_K783yb7_Dn7D6EyRkfJq3toCKeExu811Gl9BEPOAYkvdXolh5hDhH759kTo1zAF8f1NPv-6eO3yy_59uvnq8vNNtdlKWIOIKCSINtWFC0rpRFSVnVVSQRBO96WCpAWgnJODTd1i1oa09ZGqUJyoURxmr27r5u-6MeEITa9DXc3GdBPoRGioFDSqk7y7T9lzWvgglf_hSAZ5RUUCb7-C974aRzSc5OpIbUVCzq7R3r0IYxomv1oezXODdBmmY9mmY8mzUfDUghSwqtj1antsXvgx4FI4M0RqKCVM6MatA0PjgOjZVkXfwD8IsXm</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>TALUKDER, M. 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Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lipase - metabolism</topic><topic>Micelles</topic><topic>Molecular Weight</topic><topic>Octanes - chemistry</topic><topic>Olea</topic><topic>Olive oil</topic><topic>Polyethylene glycol</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Sodium</topic><topic>Structure-Activity Relationship</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TALUKDER, M. M. R</creatorcontrib><creatorcontrib>TAKEYAMA, T</creatorcontrib><creatorcontrib>HAYASHI, Y</creatorcontrib><creatorcontrib>WU, J. 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M. R</au><au>TAKEYAMA, T</au><au>HAYASHI, Y</au><au>WU, J. C</au><au>KAWANISHI, T</au><au>SHIMIZU, N</au><au>OGINO, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/ isooctane reverse micellar system</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>110</volume><issue>2</issue><spage>101</spage><epage>112</epage><pages>101-112</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><eissn>0273-2289</eissn><coden>ABIBDL</coden><abstract>The activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-l-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (PEG 400) is added to the reverse micelles. To understand the effect of PEG 400 on the phase behavior of the reverse micellar system, the phase diagram of AOT/ PEG 400/water/isooctane system was studied. The influences of relevant parameters on the catalytic activity in AOT/PEG 400 reverse micelles were investigated and compared with the results in the simple AOT reverse micelles. In the presence of PEG 400, the linear decreasing trend of the lipase activity with AOT concentration, which is observed in the simple AOT reverse micelles, disappeared. Enzyme entrapped in AOT/PEG reverse micelles was very stable, retaining >75% of its initial activity after 60 d, whereas the half-life in simple AOT reverse micelles was 38 d. The kinetics parameter maximum velocity (Vmax) exhibiting the temperature dependence and the activation energy obtained by Arrhenius plot was suppressed significantly by the addition of PEG 400.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>14515025</pmid><doi>10.1385/ABAB:110:2:101</doi><tpages>12</tpages></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2003-08, Vol.110 (2), p.101-112 |
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| subjects | Biological and medical sciences Biotechnology Chromobacterium - enzymology Chromobacterium viscosum Dioctyl Sulfosuccinic Acid - metabolism Enzymatic activity Enzyme Activation Enzyme Stability Enzymes Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Lipase - metabolism Micelles Molecular Weight Octanes - chemistry Olea Olive oil Polyethylene glycol Polyethylene Glycols - chemistry Polyethylene Glycols - pharmacology Sodium Structure-Activity Relationship Studies |
| title | Improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/ isooctane reverse micellar system |
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