Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection
Streptomycin (STR) and dihydrostreptomycin (DHSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. The physicochemical properties of both substances, make their determination challenging. In the present study the development of methods based on ion-pair chromatogra...
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| Veröffentlicht in: | Journal of Chromatography A 2010-10, Vol.1217 (43), p.6646-6651 |
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| creator | Gremilogianni, Aikaterini M. Megoulas, Nikolaos C. Koupparis, Michael A. |
| description | Streptomycin (STR) and
dihydrostreptomycin (DHSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. The physicochemical properties of both substances, make their determination challenging. In the present study the development of methods based on ion-pair chromatography (IPC) and on hydrophilic interaction chromatography (HILIC), for the determination of the above mentioned aminoglycosides in the range of 100–1000
μg
L
−1 is described. The two methods were validated according to EU requirements for residues in food. The recoveries for the IPC method were 69.3% and 56.5% of STR and DHSTR, respectively, and for HILIC method 85.5% and 72.3%, respectively. The intra- and inter-day precision, studied at 100, 200 and 300
μg
kg
−1 levels in milk samples, gave %RSD
≤
13 for both methods. LOQs for the HILIC method were 14
μg
kg
−1 for both analytes and for the IPC method were 109 and 31
μg
kg
−1, for STR and DHSTR, respectively. The sensitivity of the HILIC method is 80 and 210 times greater than that of the ICP method, for STR and DHSTR, respectively. |
| doi_str_mv | 10.1016/j.chroma.2010.04.059 |
| format | Article |
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dihydrostreptomycin (DHSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. The physicochemical properties of both substances, make their determination challenging. In the present study the development of methods based on ion-pair chromatography (IPC) and on hydrophilic interaction chromatography (HILIC), for the determination of the above mentioned aminoglycosides in the range of 100–1000
μg
L
−1 is described. The two methods were validated according to EU requirements for residues in food. The recoveries for the IPC method were 69.3% and 56.5% of STR and DHSTR, respectively, and for HILIC method 85.5% and 72.3%, respectively. The intra- and inter-day precision, studied at 100, 200 and 300
μg
kg
−1 levels in milk samples, gave %RSD
≤
13 for both methods. LOQs for the HILIC method were 14
μg
kg
−1 for both analytes and for the IPC method were 109 and 31
μg
kg
−1, for STR and DHSTR, respectively. The sensitivity of the HILIC method is 80 and 210 times greater than that of the ICP method, for STR and DHSTR, respectively.</description><identifier>ISSN: 0021-9673</identifier><identifier>EISSN: 1873-3778</identifier><identifier>DOI: 10.1016/j.chroma.2010.04.059</identifier><identifier>PMID: 20546761</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Chromatography, Liquid - methods ; Dihydrostreptomycin ; Dihydrostreptomycin Sulfate - analysis ; Dihydrostreptomycin Sulfate - chemistry ; Food Analysis - methods ; Food industries ; Fundamental and applied biological sciences. Psychology ; HILIC ; Hydrophobic and Hydrophilic Interactions ; Ion pair liquid chromatography ; LC–MS ; Mass Spectrometry - methods ; Milk ; Milk - chemistry ; Milk and cheese industries. Ice creams ; Reproducibility of Results ; Sensitivity and Specificity ; Streptomycin ; Streptomycin - analysis ; Streptomycin - chemistry</subject><ispartof>Journal of Chromatography A, 2010-10, Vol.1217 (43), p.6646-6651</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-359ad1eef92a6bd15776c09efed0a62a1a3f1f32cbda0167e45d7e8478e7945c3</citedby><cites>FETCH-LOGICAL-c513t-359ad1eef92a6bd15776c09efed0a62a1a3f1f32cbda0167e45d7e8478e7945c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021967310005625$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23340818$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20546761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gremilogianni, Aikaterini M.</creatorcontrib><creatorcontrib>Megoulas, Nikolaos C.</creatorcontrib><creatorcontrib>Koupparis, Michael A.</creatorcontrib><title>Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>Streptomycin (STR) and
dihydrostreptomycin (DHSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. The physicochemical properties of both substances, make their determination challenging. In the present study the development of methods based on ion-pair chromatography (IPC) and on hydrophilic interaction chromatography (HILIC), for the determination of the above mentioned aminoglycosides in the range of 100–1000
μg
L
−1 is described. The two methods were validated according to EU requirements for residues in food. The recoveries for the IPC method were 69.3% and 56.5% of STR and DHSTR, respectively, and for HILIC method 85.5% and 72.3%, respectively. The intra- and inter-day precision, studied at 100, 200 and 300
μg
kg
−1 levels in milk samples, gave %RSD
≤
13 for both methods. LOQs for the HILIC method were 14
μg
kg
−1 for both analytes and for the IPC method were 109 and 31
μg
kg
−1, for STR and DHSTR, respectively. The sensitivity of the HILIC method is 80 and 210 times greater than that of the ICP method, for STR and DHSTR, respectively.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Dihydrostreptomycin</subject><subject>Dihydrostreptomycin Sulfate - analysis</subject><subject>Dihydrostreptomycin Sulfate - chemistry</subject><subject>Food Analysis - methods</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HILIC</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Ion pair liquid chromatography</subject><subject>LC–MS</subject><subject>Mass Spectrometry - methods</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Streptomycin</subject><subject>Streptomycin - analysis</subject><subject>Streptomycin - chemistry</subject><issn>0021-9673</issn><issn>1873-3778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFv1DAQhS0EokvhHyDwBfWUxY7jOLkgoYpSpEocoGdr1h43XpI4tbOV9k_xG3GaBcSFk6XRN_Oe3yPkNWdbznj9fr81XQwDbEuWR6zaMtk-IRveKFEIpZqnZMNYyYu2VuKMvEhpzxhXTJXPyVnJZFWrmm_Iz-ujjWHqfO8N9eOMEczsw0gfEl2eCXykvb8_eEtXvTncRZi6I3Uh0rlDajFvDX6Ex73gaJojTnMYjsaPFEZLre8WlX_mEZO3B8wqIx18_4PuIKGl-cIAKdE0oZmzHM4xG1skHm29JM8c9Alfnd5zcnv16fvldXHz9fOXy483hZFczIWQLViO6NoS6p3lUqnasBYdWgZ1CRyE406UZmchZ6mwklZhU6kGVVtJI87JxXp3iuE-u5z14JPBvocRwyHpphGMl1LKTFYrafIHU0Snp-gHiEfNmV6K0nu9BqeXojSrdC4qr705CRx2A9o_S7-bycC7EwDJQO8ijManv5wQFWt4k7m3K-cgaLiLmbn9lpWyv6ZVdS0y8WElMAf24DHqZDyOBq2POVVtg_-_118EkMLP</recordid><startdate>20101022</startdate><enddate>20101022</enddate><creator>Gremilogianni, Aikaterini M.</creator><creator>Megoulas, Nikolaos C.</creator><creator>Koupparis, Michael A.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20101022</creationdate><title>Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection</title><author>Gremilogianni, Aikaterini M. ; Megoulas, Nikolaos C. ; Koupparis, Michael A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-359ad1eef92a6bd15776c09efed0a62a1a3f1f32cbda0167e45d7e8478e7945c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>Dihydrostreptomycin</topic><topic>Dihydrostreptomycin Sulfate - analysis</topic><topic>Dihydrostreptomycin Sulfate - chemistry</topic><topic>Food Analysis - methods</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HILIC</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Ion pair liquid chromatography</topic><topic>LC–MS</topic><topic>Mass Spectrometry - methods</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Streptomycin</topic><topic>Streptomycin - analysis</topic><topic>Streptomycin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gremilogianni, Aikaterini M.</creatorcontrib><creatorcontrib>Megoulas, Nikolaos C.</creatorcontrib><creatorcontrib>Koupparis, Michael A.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gremilogianni, Aikaterini M.</au><au>Megoulas, Nikolaos C.</au><au>Koupparis, Michael A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2010-10-22</date><risdate>2010</risdate><volume>1217</volume><issue>43</issue><spage>6646</spage><epage>6651</epage><pages>6646-6651</pages><issn>0021-9673</issn><eissn>1873-3778</eissn><coden>JOCRAM</coden><abstract>Streptomycin (STR) and
dihydrostreptomycin (DHSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. The physicochemical properties of both substances, make their determination challenging. In the present study the development of methods based on ion-pair chromatography (IPC) and on hydrophilic interaction chromatography (HILIC), for the determination of the above mentioned aminoglycosides in the range of 100–1000
μg
L
−1 is described. The two methods were validated according to EU requirements for residues in food. The recoveries for the IPC method were 69.3% and 56.5% of STR and DHSTR, respectively, and for HILIC method 85.5% and 72.3%, respectively. The intra- and inter-day precision, studied at 100, 200 and 300
μg
kg
−1 levels in milk samples, gave %RSD
≤
13 for both methods. LOQs for the HILIC method were 14
μg
kg
−1 for both analytes and for the IPC method were 109 and 31
μg
kg
−1, for STR and DHSTR, respectively. The sensitivity of the HILIC method is 80 and 210 times greater than that of the ICP method, for STR and DHSTR, respectively.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20546761</pmid><doi>10.1016/j.chroma.2010.04.059</doi><tpages>6</tpages></addata></record> |
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| issn | 0021-9673 1873-3778 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Chromatography, Liquid - methods Dihydrostreptomycin Dihydrostreptomycin Sulfate - analysis Dihydrostreptomycin Sulfate - chemistry Food Analysis - methods Food industries Fundamental and applied biological sciences. Psychology HILIC Hydrophobic and Hydrophilic Interactions Ion pair liquid chromatography LC–MS Mass Spectrometry - methods Milk Milk - chemistry Milk and cheese industries. Ice creams Reproducibility of Results Sensitivity and Specificity Streptomycin Streptomycin - analysis Streptomycin - chemistry |
| title | Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection |
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