A simple method for producing tetraploid porcine parthenogenetic embryos

The objective was to produce porcine tetraploid parthenogenetic embryos using cytochalasin B, which inhibits polar body extrusion. Porcine cumulus-enclosed oocytes aspirated from antral follicles were cultured for 51 h, and treated with cytochalasin B from 35 h to 42 h after the start of culture. Af...

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Veröffentlicht in:	Theriogenology 2011-09, Vol.76 (4), p.598-606
Hauptverfasser:	Sembon, S, Fuchimoto, D, Iwamoto, M, Suzuki, S, Yoshioka, K, Onishi, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chi-Square Distribution chromosome number chromosomes Cytochalasin cytochalasin B Cytochalasin B - pharmacology Electric Stimulation - methods electrical treatment Embryo Culture Techniques - methods Embryo Culture Techniques - veterinary Embryo development embryogenesis Embryonic Development - physiology Female fluorescence in situ hybridization In Situ Hybridization, Fluorescence - veterinary Meiosis - physiology oocytes Oocytes - cytology Oocytes - physiology Parthenogenesis Parthenogenesis - physiology Pig Pregnancy pronucleus swine Swine - embryology Swine - genetics Swine - physiology Tetraploid Tetraploidy
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