Effect of a combined treatment with growth hormone and melatonin in the cardiological aging on male SAMP8 mice
The effect of a chronic combined treatment with growth hormone (GH) plus melatonin (Mel) on different age-related processes in cytosolic and nuclear fractions of hearts from SAMP8 mice (2 and 10 months) has been investigated. The parameters studied have been messenger RNA expressions of IL-1, IL-10,...
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| Veröffentlicht in: | The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2011-08, Vol.66 (8), p.823-834 |
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| container_title | The journals of gerontology. Series A, Biological sciences and medical sciences |
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| creator | Forman, Katherine Vara, Elena García, Cruz Kireev, Roman Cuesta, Sara Escames, Germaine Tresguerres, J A F |
| description | The effect of a chronic combined treatment with growth hormone (GH) plus melatonin (Mel) on different age-related processes in cytosolic and nuclear fractions of hearts from SAMP8 mice (2 and 10 months) has been investigated. The parameters studied have been messenger RNA expressions of IL-1, IL-10, NFkBp50, NFkBp52, TNFα, eNOS, iNOS, HO-1, HO-2, BAD, BAX, and Bcl2 and protein expressions of iNOS, eNOS, TNFα, IL-1, IL-10, NFkBp50, NFKbp52, and caspase activity (3 and 9). Our results supported the existence of a proapoptotic and oxidative status together with inflammatory processes in the heart of old mice, with increases of inflammatory cytokines, caspase activity, HO-1, BAX, NFkBp50, and NFkBp52 and decreases of eNOS and Bcl2. Also, we were able to observe the translocation of NFkB to nuclei. The combined treatment was able to partially reduce the incidence of these deleterious changes, showing differences with the separated treatments with GH and Mel as were investigated in previous articles from our group. |
| doi_str_mv | 10.1093/gerona/glr083 |
| format | Article |
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The parameters studied have been messenger RNA expressions of IL-1, IL-10, NFkBp50, NFkBp52, TNFα, eNOS, iNOS, HO-1, HO-2, BAD, BAX, and Bcl2 and protein expressions of iNOS, eNOS, TNFα, IL-1, IL-10, NFkBp50, NFKbp52, and caspase activity (3 and 9). Our results supported the existence of a proapoptotic and oxidative status together with inflammatory processes in the heart of old mice, with increases of inflammatory cytokines, caspase activity, HO-1, BAX, NFkBp50, and NFkBp52 and decreases of eNOS and Bcl2. Also, we were able to observe the translocation of NFkB to nuclei. The combined treatment was able to partially reduce the incidence of these deleterious changes, showing differences with the separated treatments with GH and Mel as were investigated in previous articles from our group.</description><identifier>ISSN: 1079-5006</identifier><identifier>EISSN: 1758-535X</identifier><identifier>DOI: 10.1093/gerona/glr083</identifier><identifier>PMID: 21665987</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Administration, Oral ; Aging ; Aging - metabolism ; Animals ; Apoptosis ; Biomarkers - metabolism ; Cell Nucleus - metabolism ; Cytosol - metabolism ; Drug Administration Schedule ; Drug Synergism ; Growth hormones ; Heart ; Heme Oxygenase (Decyclizing) - genetics ; Heme Oxygenase (Decyclizing) - metabolism ; Human Growth Hormone - administration & dosage ; Injections, Subcutaneous ; Interleukin-1 - genetics ; Interleukin-1 - metabolism ; Interleukin-10 - genetics ; Interleukin-10 - metabolism ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Male ; Melatonin ; Melatonin - administration & dosage ; Mice ; Muscle Proteins - metabolism ; Myocarditis - physiopathology ; Myocardium - metabolism ; NF-kappa B - genetics ; NF-kappa B - metabolism ; Nitric Oxide Synthase - genetics ; Nitric Oxide Synthase - metabolism ; Oxidative Stress ; RNA, Messenger - metabolism ; Rodents ; Time Factors ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>The journals of gerontology. Series A, Biological sciences and medical sciences, 2011-08, Vol.66 (8), p.823-834</ispartof><rights>Copyright Oxford University Press, UK Aug 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-18e7975266d8d3ca91282bab874480bc0ed3d299ca26eeccd9e821c2f9136f33</citedby><cites>FETCH-LOGICAL-c358t-18e7975266d8d3ca91282bab874480bc0ed3d299ca26eeccd9e821c2f9136f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21665987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Forman, Katherine</creatorcontrib><creatorcontrib>Vara, Elena</creatorcontrib><creatorcontrib>García, Cruz</creatorcontrib><creatorcontrib>Kireev, Roman</creatorcontrib><creatorcontrib>Cuesta, Sara</creatorcontrib><creatorcontrib>Escames, Germaine</creatorcontrib><creatorcontrib>Tresguerres, J A F</creatorcontrib><title>Effect of a combined treatment with growth hormone and melatonin in the cardiological aging on male SAMP8 mice</title><title>The journals of gerontology. Series A, Biological sciences and medical sciences</title><addtitle>J Gerontol A Biol Sci Med Sci</addtitle><description>The effect of a chronic combined treatment with growth hormone (GH) plus melatonin (Mel) on different age-related processes in cytosolic and nuclear fractions of hearts from SAMP8 mice (2 and 10 months) has been investigated. The parameters studied have been messenger RNA expressions of IL-1, IL-10, NFkBp50, NFkBp52, TNFα, eNOS, iNOS, HO-1, HO-2, BAD, BAX, and Bcl2 and protein expressions of iNOS, eNOS, TNFα, IL-1, IL-10, NFkBp50, NFKbp52, and caspase activity (3 and 9). Our results supported the existence of a proapoptotic and oxidative status together with inflammatory processes in the heart of old mice, with increases of inflammatory cytokines, caspase activity, HO-1, BAX, NFkBp50, and NFkBp52 and decreases of eNOS and Bcl2. Also, we were able to observe the translocation of NFkB to nuclei. The combined treatment was able to partially reduce the incidence of these deleterious changes, showing differences with the separated treatments with GH and Mel as were investigated in previous articles from our group.</description><subject>Administration, Oral</subject><subject>Aging</subject><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomarkers - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Drug Administration Schedule</subject><subject>Drug Synergism</subject><subject>Growth hormones</subject><subject>Heart</subject><subject>Heme Oxygenase (Decyclizing) - genetics</subject><subject>Heme Oxygenase (Decyclizing) - metabolism</subject><subject>Human Growth Hormone - administration & dosage</subject><subject>Injections, Subcutaneous</subject><subject>Interleukin-1 - genetics</subject><subject>Interleukin-1 - metabolism</subject><subject>Interleukin-10 - genetics</subject><subject>Interleukin-10 - metabolism</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Male</subject><subject>Melatonin</subject><subject>Melatonin - administration & dosage</subject><subject>Mice</subject><subject>Muscle Proteins - metabolism</subject><subject>Myocarditis - physiopathology</subject><subject>Myocardium - metabolism</subject><subject>NF-kappa B - genetics</subject><subject>NF-kappa B - metabolism</subject><subject>Nitric Oxide Synthase - genetics</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Oxidative Stress</subject><subject>RNA, Messenger - metabolism</subject><subject>Rodents</subject><subject>Time Factors</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>1079-5006</issn><issn>1758-535X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtLAzEUhYMovpduJbhxNZpHM5Msi9QHKAp24W7IJHemkUlSMynFf--UVhdeLpy7-DhczkHogpIbShS_7SDFoG-7PhHJ99AxrYQsBBcf--NNKlUIQsojdDIMn2Qzgh2iI0bLUihZHaMwa1swGccWa2yib1wAi3MCnT2EjNcuL3CX4nqURUw-BsA6WOyh1zkGF_C4eQHY6GRd7GPnjO6x7lzocAzY6x7w-_TlTWLvDJyhg1b3A5zv9BTN72fzu8fi-fXh6W76XBguZC6ohEpVgpWllZYbrSiTrNGNrCYTSRpDwHLLlDKalQDGWAWSUcNaRXnZcn6Krre2yxS_VjDk2rvBQN_rAHE11FJSIkvCxEhe_SM_4yqF8bcNJLgik8kIFVvIpDgMCdp6mZzX6bumpN60UG9bqLctjPzlznTVeLB_9G_s_AebKIUl</recordid><startdate>201108</startdate><enddate>201108</enddate><creator>Forman, Katherine</creator><creator>Vara, Elena</creator><creator>García, Cruz</creator><creator>Kireev, Roman</creator><creator>Cuesta, Sara</creator><creator>Escames, Germaine</creator><creator>Tresguerres, J A F</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>201108</creationdate><title>Effect of a combined treatment with growth hormone and melatonin in the cardiological aging on male SAMP8 mice</title><author>Forman, Katherine ; Vara, Elena ; García, Cruz ; Kireev, Roman ; Cuesta, Sara ; Escames, Germaine ; Tresguerres, J A F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-18e7975266d8d3ca91282bab874480bc0ed3d299ca26eeccd9e821c2f9136f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Administration, Oral</topic><topic>Aging</topic><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomarkers - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Drug Administration Schedule</topic><topic>Drug Synergism</topic><topic>Growth hormones</topic><topic>Heart</topic><topic>Heme Oxygenase (Decyclizing) - genetics</topic><topic>Heme Oxygenase (Decyclizing) - metabolism</topic><topic>Human Growth Hormone - administration & dosage</topic><topic>Injections, Subcutaneous</topic><topic>Interleukin-1 - genetics</topic><topic>Interleukin-1 - metabolism</topic><topic>Interleukin-10 - genetics</topic><topic>Interleukin-10 - metabolism</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Male</topic><topic>Melatonin</topic><topic>Melatonin - administration & dosage</topic><topic>Mice</topic><topic>Muscle Proteins - metabolism</topic><topic>Myocarditis - physiopathology</topic><topic>Myocardium - metabolism</topic><topic>NF-kappa B - genetics</topic><topic>NF-kappa B - metabolism</topic><topic>Nitric Oxide Synthase - genetics</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Oxidative Stress</topic><topic>RNA, Messenger - metabolism</topic><topic>Rodents</topic><topic>Time Factors</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forman, Katherine</creatorcontrib><creatorcontrib>Vara, Elena</creatorcontrib><creatorcontrib>García, Cruz</creatorcontrib><creatorcontrib>Kireev, Roman</creatorcontrib><creatorcontrib>Cuesta, Sara</creatorcontrib><creatorcontrib>Escames, Germaine</creatorcontrib><creatorcontrib>Tresguerres, J A F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The journals of gerontology. 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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Administration, Oral Aging Aging - metabolism Animals Apoptosis Biomarkers - metabolism Cell Nucleus - metabolism Cytosol - metabolism Drug Administration Schedule Drug Synergism Growth hormones Heart Heme Oxygenase (Decyclizing) - genetics Heme Oxygenase (Decyclizing) - metabolism Human Growth Hormone - administration & dosage Injections, Subcutaneous Interleukin-1 - genetics Interleukin-1 - metabolism Interleukin-10 - genetics Interleukin-10 - metabolism Isoenzymes - genetics Isoenzymes - metabolism Male Melatonin Melatonin - administration & dosage Mice Muscle Proteins - metabolism Myocarditis - physiopathology Myocardium - metabolism NF-kappa B - genetics NF-kappa B - metabolism Nitric Oxide Synthase - genetics Nitric Oxide Synthase - metabolism Oxidative Stress RNA, Messenger - metabolism Rodents Time Factors Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism |
| title | Effect of a combined treatment with growth hormone and melatonin in the cardiological aging on male SAMP8 mice |
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