The Ag+–G interaction inhibits the electrocatalytic oxidation of guanine – A novel mechanism for Ag+ detection
The heavy metal ions–nucleobases interaction is an important research topic in environmental and biochemical analysis. The presence of the silver ion (Ag+) may influence the formation of oxidation intermediate and the electrocatalytic oxidation activity of guanine (G), since Ag+ can interact with gu...
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Veröffentlicht in: | Talanta (Oxford) 2011-09, Vol.85 (3), p.1603-1608 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The heavy metal ions–nucleobases interaction is an important research topic in environmental and biochemical analysis. The presence of the silver ion (Ag+) may influence the formation of oxidation intermediate and the electrocatalytic oxidation activity of guanine (G), since Ag+ can interact with guanine at the binding sites which are involved in the electrocatalytic oxidation reaction of guanine. According to this principle, a new electrochemical sensor for indirectly detecting Ag+ based on the interaction of Ag+ with isolated guanine base using differential pulse voltammetry (DPV) was constructed. Among the heavy metal ions examined, only Ag+ showed the strongest inhibitory effect on the electrocatalytic oxidation of guanine at the multi-walled carbon nanotubes modified glassy carbon electrode (CNTs/GC). And the quantitative study of Ag+ based on Ag+–G sensing system gave a linear range from 100nM to 2.5μM with a detection limit of 30nM. In addition, this modified electrode had very good reproducibility and stability. The developed electrochemical method is an ideal tool for Ag+ detection with some merits including remarkable simplicity, low-cost, and no requirement for probe preparation. |
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ISSN: | 0039-9140 1873-3573 |
DOI: | 10.1016/j.talanta.2011.06.061 |