Altered expression of Dscam in temporal lobe tissue from human and experimental animals
Down syndrome cell adhesion molecule (Dscam) is a neural adhesion molecule that plays an essential role in the establishment of neural circuits. Considerable evidence suggests that Dscam is required for axon guidance and dendritic arborization. Our aim was to investigate the expression of Dscam in t...
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| Veröffentlicht in: | Synapse (New York, N.Y.) N.Y.), 2011-10, Vol.65 (10), p.975-982 |
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| creator | Shen, Lan Xiao, Zheng Pan, Yumin Fang, Min Li, Chengshan Chen, Dan Wang, Liang Xi, Zhiqin Xiao, Fei Wang, Xuefeng |
| description | Down syndrome cell adhesion molecule (Dscam) is a neural adhesion molecule that plays an essential role in the establishment of neural circuits. Considerable evidence suggests that Dscam is required for axon guidance and dendritic arborization. Our aim was to investigate the expression of Dscam in the temporal lobes of patients with intractable epilepsy (IE) and of experimental animals. In this study, we used immunohistochemistry, immunofluorescence, and western blotting to examine Dscam expression in thirty‐five surgical samples from brains of IE patients and 15 control brain samples. We also measured the levels of Dscam during the entire epileptic process in a rat model of temporal lobe epilepsy. Dscam expression in IE patients was significantly higher compared with that in the controls. In addition, Dscam was also highly expressed in the rat brain during the different phases of the epileptic process. It is the first time to find abnormal expression of Dscam in the brain tissues in patients with IE. And this finding provides an experimental evidence for the study of neuronal circuit remodeling and synaptic plasticity in IE, furthermore, our results also suggest that Dscam may be involved in the generation and the development of IE. Synapse, 2011. © 2011 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/syn.20924 |
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Considerable evidence suggests that Dscam is required for axon guidance and dendritic arborization. Our aim was to investigate the expression of Dscam in the temporal lobes of patients with intractable epilepsy (IE) and of experimental animals. In this study, we used immunohistochemistry, immunofluorescence, and western blotting to examine Dscam expression in thirty‐five surgical samples from brains of IE patients and 15 control brain samples. We also measured the levels of Dscam during the entire epileptic process in a rat model of temporal lobe epilepsy. Dscam expression in IE patients was significantly higher compared with that in the controls. In addition, Dscam was also highly expressed in the rat brain during the different phases of the epileptic process. It is the first time to find abnormal expression of Dscam in the brain tissues in patients with IE. And this finding provides an experimental evidence for the study of neuronal circuit remodeling and synaptic plasticity in IE, furthermore, our results also suggest that Dscam may be involved in the generation and the development of IE. Synapse, 2011. © 2011 Wiley‐Liss, Inc.</description><identifier>ISSN: 0887-4476</identifier><identifier>ISSN: 1098-2396</identifier><identifier>EISSN: 1098-2396</identifier><identifier>DOI: 10.1002/syn.20924</identifier><identifier>PMID: 21360594</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adolescent ; Adult ; Animal models ; Animals ; Axon guidance ; Brain ; Cell adhesion molecules ; Cell Adhesion Molecules - metabolism ; Child ; Circuits ; Dendritic branching ; Disease Models, Animal ; Dscam ; DSCAM protein ; Epilepsy ; Epilepsy, Temporal Lobe - chemically induced ; Epilepsy, Temporal Lobe - etiology ; Epilepsy, Temporal Lobe - metabolism ; Female ; Humans ; Immunofluorescence ; Immunohistochemistry ; intractable epilepsy ; Male ; Middle Aged ; mossy fiber sprouting ; Muscarinic Agonists - toxicity ; Nerve Tissue Proteins - biosynthesis ; Nerve Tissue Proteins - genetics ; Neural cell adhesion molecule ; Neural networks ; Neuronal Plasticity - genetics ; Pilocarpine - toxicity ; Plasticity (synaptic) ; Rats ; Rats, Sprague-Dawley ; Synapses - genetics ; Synaptogenesis ; Temporal lobe ; Temporal Lobe - metabolism ; Temporal Lobe - physiopathology ; Western blotting ; Young Adult</subject><ispartof>Synapse (New York, N.Y.), 2011-10, Vol.65 (10), p.975-982</ispartof><rights>Copyright © 2011 Wiley‐Liss, Inc.</rights><rights>Copyright © 2011 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3954-8ca891a1cc22fefc1d10475b2db24aeadb1a152512d10266b279cde9fb5fdd293</citedby><cites>FETCH-LOGICAL-c3954-8ca891a1cc22fefc1d10475b2db24aeadb1a152512d10266b279cde9fb5fdd293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fsyn.20924$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fsyn.20924$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21360594$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Lan</creatorcontrib><creatorcontrib>Xiao, Zheng</creatorcontrib><creatorcontrib>Pan, Yumin</creatorcontrib><creatorcontrib>Fang, Min</creatorcontrib><creatorcontrib>Li, Chengshan</creatorcontrib><creatorcontrib>Chen, Dan</creatorcontrib><creatorcontrib>Wang, Liang</creatorcontrib><creatorcontrib>Xi, Zhiqin</creatorcontrib><creatorcontrib>Xiao, Fei</creatorcontrib><creatorcontrib>Wang, Xuefeng</creatorcontrib><title>Altered expression of Dscam in temporal lobe tissue from human and experimental animals</title><title>Synapse (New York, N.Y.)</title><addtitle>Synapse</addtitle><description>Down syndrome cell adhesion molecule (Dscam) is a neural adhesion molecule that plays an essential role in the establishment of neural circuits. Considerable evidence suggests that Dscam is required for axon guidance and dendritic arborization. Our aim was to investigate the expression of Dscam in the temporal lobes of patients with intractable epilepsy (IE) and of experimental animals. In this study, we used immunohistochemistry, immunofluorescence, and western blotting to examine Dscam expression in thirty‐five surgical samples from brains of IE patients and 15 control brain samples. We also measured the levels of Dscam during the entire epileptic process in a rat model of temporal lobe epilepsy. Dscam expression in IE patients was significantly higher compared with that in the controls. In addition, Dscam was also highly expressed in the rat brain during the different phases of the epileptic process. It is the first time to find abnormal expression of Dscam in the brain tissues in patients with IE. And this finding provides an experimental evidence for the study of neuronal circuit remodeling and synaptic plasticity in IE, furthermore, our results also suggest that Dscam may be involved in the generation and the development of IE. Synapse, 2011. © 2011 Wiley‐Liss, Inc.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Animal models</subject><subject>Animals</subject><subject>Axon guidance</subject><subject>Brain</subject><subject>Cell adhesion molecules</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Child</subject><subject>Circuits</subject><subject>Dendritic branching</subject><subject>Disease Models, Animal</subject><subject>Dscam</subject><subject>DSCAM protein</subject><subject>Epilepsy</subject><subject>Epilepsy, Temporal Lobe - chemically induced</subject><subject>Epilepsy, Temporal Lobe - etiology</subject><subject>Epilepsy, Temporal Lobe - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Immunohistochemistry</subject><subject>intractable epilepsy</subject><subject>Male</subject><subject>Middle Aged</subject><subject>mossy fiber sprouting</subject><subject>Muscarinic Agonists - toxicity</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Neural cell adhesion molecule</subject><subject>Neural networks</subject><subject>Neuronal Plasticity - genetics</subject><subject>Pilocarpine - toxicity</subject><subject>Plasticity (synaptic)</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Synapses - genetics</subject><subject>Synaptogenesis</subject><subject>Temporal lobe</subject><subject>Temporal Lobe - metabolism</subject><subject>Temporal Lobe - physiopathology</subject><subject>Western blotting</subject><subject>Young Adult</subject><issn>0887-4476</issn><issn>1098-2396</issn><issn>1098-2396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EgvJY8APIO2CR4kdeXqJCW6QIhABVsLEcZyICeWEnov17DKHdwWoWc-7RzEXomJIxJYRd2FU9ZkQwfwuNKBGxx7gIt9GIxHHk-X4U7qF9a98IIZwSfxftMcpDEgh_hBaXZQcGMgzL1oC1RVPjJsdXVqsKFzXuoGobo0pcNingrrC2B5ybpsKvfaVqrOqfKJiigrpznKqLSpX2EO3kbsDR7zxAT9Prx8ncS-5mN5PLxNNcBL4XaxULqqjWjOWQa5q5A6MgZVnKfAUqS90yYAFlbsHCMGWR0BmIPA3yLGOCH6DTwdua5qMH28mqsBrKUtXQ9FbG8XdDfkAdefYvSQmNRMgI5w49H1BtGmsN5LJ1_ymzcpD89knXuPxp3LEnv9o-rSDbkOuKHXAxAJ9FCau_TfLh-Xat9IZEYTtYbhLKvMsw4lEgF7czef8ipvMkmUjGvwAsnZpy</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Shen, Lan</creator><creator>Xiao, Zheng</creator><creator>Pan, Yumin</creator><creator>Fang, Min</creator><creator>Li, Chengshan</creator><creator>Chen, Dan</creator><creator>Wang, Liang</creator><creator>Xi, Zhiqin</creator><creator>Xiao, Fei</creator><creator>Wang, Xuefeng</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>201110</creationdate><title>Altered expression of Dscam in temporal lobe tissue from human and experimental animals</title><author>Shen, Lan ; Xiao, Zheng ; Pan, Yumin ; Fang, Min ; Li, Chengshan ; Chen, Dan ; Wang, Liang ; Xi, Zhiqin ; Xiao, Fei ; Wang, Xuefeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3954-8ca891a1cc22fefc1d10475b2db24aeadb1a152512d10266b279cde9fb5fdd293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Animal models</topic><topic>Animals</topic><topic>Axon guidance</topic><topic>Brain</topic><topic>Cell adhesion molecules</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Child</topic><topic>Circuits</topic><topic>Dendritic branching</topic><topic>Disease Models, Animal</topic><topic>Dscam</topic><topic>DSCAM protein</topic><topic>Epilepsy</topic><topic>Epilepsy, Temporal Lobe - chemically induced</topic><topic>Epilepsy, Temporal Lobe - etiology</topic><topic>Epilepsy, Temporal Lobe - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Immunohistochemistry</topic><topic>intractable epilepsy</topic><topic>Male</topic><topic>Middle Aged</topic><topic>mossy fiber sprouting</topic><topic>Muscarinic Agonists - toxicity</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Neural cell adhesion molecule</topic><topic>Neural networks</topic><topic>Neuronal Plasticity - genetics</topic><topic>Pilocarpine - toxicity</topic><topic>Plasticity (synaptic)</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Synapses - genetics</topic><topic>Synaptogenesis</topic><topic>Temporal lobe</topic><topic>Temporal Lobe - metabolism</topic><topic>Temporal Lobe - physiopathology</topic><topic>Western blotting</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Lan</creatorcontrib><creatorcontrib>Xiao, Zheng</creatorcontrib><creatorcontrib>Pan, Yumin</creatorcontrib><creatorcontrib>Fang, Min</creatorcontrib><creatorcontrib>Li, Chengshan</creatorcontrib><creatorcontrib>Chen, Dan</creatorcontrib><creatorcontrib>Wang, Liang</creatorcontrib><creatorcontrib>Xi, Zhiqin</creatorcontrib><creatorcontrib>Xiao, Fei</creatorcontrib><creatorcontrib>Wang, Xuefeng</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Synapse (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Lan</au><au>Xiao, Zheng</au><au>Pan, Yumin</au><au>Fang, Min</au><au>Li, Chengshan</au><au>Chen, Dan</au><au>Wang, Liang</au><au>Xi, Zhiqin</au><au>Xiao, Fei</au><au>Wang, Xuefeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered expression of Dscam in temporal lobe tissue from human and experimental animals</atitle><jtitle>Synapse (New York, N.Y.)</jtitle><addtitle>Synapse</addtitle><date>2011-10</date><risdate>2011</risdate><volume>65</volume><issue>10</issue><spage>975</spage><epage>982</epage><pages>975-982</pages><issn>0887-4476</issn><issn>1098-2396</issn><eissn>1098-2396</eissn><abstract>Down syndrome cell adhesion molecule (Dscam) is a neural adhesion molecule that plays an essential role in the establishment of neural circuits. Considerable evidence suggests that Dscam is required for axon guidance and dendritic arborization. Our aim was to investigate the expression of Dscam in the temporal lobes of patients with intractable epilepsy (IE) and of experimental animals. In this study, we used immunohistochemistry, immunofluorescence, and western blotting to examine Dscam expression in thirty‐five surgical samples from brains of IE patients and 15 control brain samples. We also measured the levels of Dscam during the entire epileptic process in a rat model of temporal lobe epilepsy. Dscam expression in IE patients was significantly higher compared with that in the controls. In addition, Dscam was also highly expressed in the rat brain during the different phases of the epileptic process. It is the first time to find abnormal expression of Dscam in the brain tissues in patients with IE. And this finding provides an experimental evidence for the study of neuronal circuit remodeling and synaptic plasticity in IE, furthermore, our results also suggest that Dscam may be involved in the generation and the development of IE. Synapse, 2011. © 2011 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>21360594</pmid><doi>10.1002/syn.20924</doi><tpages>8</tpages></addata></record> |
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| subjects | Adolescent Adult Animal models Animals Axon guidance Brain Cell adhesion molecules Cell Adhesion Molecules - metabolism Child Circuits Dendritic branching Disease Models, Animal Dscam DSCAM protein Epilepsy Epilepsy, Temporal Lobe - chemically induced Epilepsy, Temporal Lobe - etiology Epilepsy, Temporal Lobe - metabolism Female Humans Immunofluorescence Immunohistochemistry intractable epilepsy Male Middle Aged mossy fiber sprouting Muscarinic Agonists - toxicity Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Neural cell adhesion molecule Neural networks Neuronal Plasticity - genetics Pilocarpine - toxicity Plasticity (synaptic) Rats Rats, Sprague-Dawley Synapses - genetics Synaptogenesis Temporal lobe Temporal Lobe - metabolism Temporal Lobe - physiopathology Western blotting Young Adult |
| title | Altered expression of Dscam in temporal lobe tissue from human and experimental animals |
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