Sodium nitrite induces epithelial-mesenchymal transition of SMMC-7721 cells
This study is to find out the induction by sodium nitrite of epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cells, SMMC-7721. After treatment of SMMC-7721 with 0.25 - 25 mmol.L-1 sodium nitrite for 48 h, the assays used include enzyme-linked immunosorbent assay (ELISA) for...
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| Veröffentlicht in: | Yao hsüeh hsüeh pao 2011-05, Vol.46 (5), p.507-512 |
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| creator | Wang, Yu-dong Fu, Jian-min Shi, Qi Li, Yan-hong Huang-Fu, Chao-shen |
| description | This study is to find out the induction by sodium nitrite of epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cells, SMMC-7721. After treatment of SMMC-7721 with 0.25 - 25 mmol.L-1 sodium nitrite for 48 h, the assays used include enzyme-linked immunosorbent assay (ELISA) for evaluation of TGF-beta1, IL-6 and IL-8 level in the conditioned medium, phase-contrast microscopy for morphology observation, and scratch wound healing as well as transwell migration assays for measurement of migration and metastatic potential. Additionally, the hallmarks of EMT, p-AKT and its downstream signaling molecules were examined by Western blotting. The results showed that TGF-beta1 secreted by SMMC-7721 elevated significantly in a dose-dependent fashion, whereas the increased IL-8 and IL-6 did not show dose-dependent response. The EMT was induced by exposure of SMMC-7721 with 0.25 mmol.L-1 of sodium nitrite, which was characterized by increased level of Vimentin, decreased E-cadherin and elevated activity of migration and metastatic potential. The results suggest that sodium nitrite could induce SMMC-7721 EMT by increased secretion of TGF-beta1 and IL-8. |
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After treatment of SMMC-7721 with 0.25 - 25 mmol.L-1 sodium nitrite for 48 h, the assays used include enzyme-linked immunosorbent assay (ELISA) for evaluation of TGF-beta1, IL-6 and IL-8 level in the conditioned medium, phase-contrast microscopy for morphology observation, and scratch wound healing as well as transwell migration assays for measurement of migration and metastatic potential. Additionally, the hallmarks of EMT, p-AKT and its downstream signaling molecules were examined by Western blotting. The results showed that TGF-beta1 secreted by SMMC-7721 elevated significantly in a dose-dependent fashion, whereas the increased IL-8 and IL-6 did not show dose-dependent response. The EMT was induced by exposure of SMMC-7721 with 0.25 mmol.L-1 of sodium nitrite, which was characterized by increased level of Vimentin, decreased E-cadherin and elevated activity of migration and metastatic potential. The results suggest that sodium nitrite could induce SMMC-7721 EMT by increased secretion of TGF-beta1 and IL-8.</description><identifier>ISSN: 0513-4870</identifier><identifier>PMID: 21800536</identifier><language>chi ; jpn</language><publisher>China</publisher><subject>Cadherins - metabolism ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; Cell Line, Tumor ; Cell Movement ; Dose-Response Relationship, Drug ; Epithelial-Mesenchymal Transition - drug effects ; Humans ; Interleukin-6 - secretion ; Interleukin-8 - secretion ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Neoplasm Invasiveness ; NF-kappa B - metabolism ; Proto-Oncogene Proteins c-akt - metabolism ; Sodium Nitrite - administration & dosage ; Sodium Nitrite - pharmacology ; Transforming Growth Factor beta1 - secretion ; Twist-Related Protein 1 - metabolism ; Vimentin - metabolism</subject><ispartof>Yao hsüeh hsüeh pao, 2011-05, Vol.46 (5), p.507-512</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21800536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yu-dong</creatorcontrib><creatorcontrib>Fu, Jian-min</creatorcontrib><creatorcontrib>Shi, Qi</creatorcontrib><creatorcontrib>Li, Yan-hong</creatorcontrib><creatorcontrib>Huang-Fu, Chao-shen</creatorcontrib><title>Sodium nitrite induces epithelial-mesenchymal transition of SMMC-7721 cells</title><title>Yao hsüeh hsüeh pao</title><addtitle>Yao Xue Xue Bao</addtitle><description>This study is to find out the induction by sodium nitrite of epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cells, SMMC-7721. After treatment of SMMC-7721 with 0.25 - 25 mmol.L-1 sodium nitrite for 48 h, the assays used include enzyme-linked immunosorbent assay (ELISA) for evaluation of TGF-beta1, IL-6 and IL-8 level in the conditioned medium, phase-contrast microscopy for morphology observation, and scratch wound healing as well as transwell migration assays for measurement of migration and metastatic potential. Additionally, the hallmarks of EMT, p-AKT and its downstream signaling molecules were examined by Western blotting. The results showed that TGF-beta1 secreted by SMMC-7721 elevated significantly in a dose-dependent fashion, whereas the increased IL-8 and IL-6 did not show dose-dependent response. The EMT was induced by exposure of SMMC-7721 with 0.25 mmol.L-1 of sodium nitrite, which was characterized by increased level of Vimentin, decreased E-cadherin and elevated activity of migration and metastatic potential. The results suggest that sodium nitrite could induce SMMC-7721 EMT by increased secretion of TGF-beta1 and IL-8.</description><subject>Cadherins - metabolism</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epithelial-Mesenchymal Transition - drug effects</subject><subject>Humans</subject><subject>Interleukin-6 - secretion</subject><subject>Interleukin-8 - secretion</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Neoplasm Invasiveness</subject><subject>NF-kappa B - metabolism</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Sodium Nitrite - administration & dosage</subject><subject>Sodium Nitrite - pharmacology</subject><subject>Transforming Growth Factor beta1 - secretion</subject><subject>Twist-Related Protein 1 - metabolism</subject><subject>Vimentin - metabolism</subject><issn>0513-4870</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1jztPwzAYRT2AaCn8BeSNyZIfceyMqOIlWjEU5siPz6pR4oTYGfrvCaJIV7rL0dW5F2hNJROk0oqu0HXOX5RWrBH6Cq0405RKUa_R22Hwce5ximWKBXBMfnaQMYyxHKGLpiM9ZEjueOpNh8tkUo4lDgkPAR_2-y1RijPsoOvyDboMpstwe-4N-nx6_Ni-kN378-v2YUdGxmUhIUjDVOW8p15oqxvFaR2YVNwu0c7LIBtQQtpgF92qoVArUFwqakXtjNig-7_dcRq-Z8il7WP-NTAJhjm3WlPFqqrhC3l3Jmfbg2_HKfZmOrX__8UPzF1VFw</recordid><startdate>201105</startdate><enddate>201105</enddate><creator>Wang, Yu-dong</creator><creator>Fu, Jian-min</creator><creator>Shi, Qi</creator><creator>Li, Yan-hong</creator><creator>Huang-Fu, Chao-shen</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201105</creationdate><title>Sodium nitrite induces epithelial-mesenchymal transition of SMMC-7721 cells</title><author>Wang, Yu-dong ; Fu, Jian-min ; Shi, Qi ; Li, Yan-hong ; Huang-Fu, Chao-shen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p125t-ff5a174cdd0d38b897206f1572b72b8cd5f59e735bfb938490e67e72570b36ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi ; jpn</language><creationdate>2011</creationdate><topic>Cadherins - metabolism</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epithelial-Mesenchymal Transition - drug effects</topic><topic>Humans</topic><topic>Interleukin-6 - secretion</topic><topic>Interleukin-8 - secretion</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Neoplasm Invasiveness</topic><topic>NF-kappa B - metabolism</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Sodium Nitrite - administration & dosage</topic><topic>Sodium Nitrite - pharmacology</topic><topic>Transforming Growth Factor beta1 - secretion</topic><topic>Twist-Related Protein 1 - metabolism</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yu-dong</creatorcontrib><creatorcontrib>Fu, Jian-min</creatorcontrib><creatorcontrib>Shi, Qi</creatorcontrib><creatorcontrib>Li, Yan-hong</creatorcontrib><creatorcontrib>Huang-Fu, Chao-shen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Yao hsüeh hsüeh pao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yu-dong</au><au>Fu, Jian-min</au><au>Shi, Qi</au><au>Li, Yan-hong</au><au>Huang-Fu, Chao-shen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sodium nitrite induces epithelial-mesenchymal transition of SMMC-7721 cells</atitle><jtitle>Yao hsüeh hsüeh pao</jtitle><addtitle>Yao Xue Xue Bao</addtitle><date>2011-05</date><risdate>2011</risdate><volume>46</volume><issue>5</issue><spage>507</spage><epage>512</epage><pages>507-512</pages><issn>0513-4870</issn><abstract>This study is to find out the induction by sodium nitrite of epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cells, SMMC-7721. After treatment of SMMC-7721 with 0.25 - 25 mmol.L-1 sodium nitrite for 48 h, the assays used include enzyme-linked immunosorbent assay (ELISA) for evaluation of TGF-beta1, IL-6 and IL-8 level in the conditioned medium, phase-contrast microscopy for morphology observation, and scratch wound healing as well as transwell migration assays for measurement of migration and metastatic potential. Additionally, the hallmarks of EMT, p-AKT and its downstream signaling molecules were examined by Western blotting. The results showed that TGF-beta1 secreted by SMMC-7721 elevated significantly in a dose-dependent fashion, whereas the increased IL-8 and IL-6 did not show dose-dependent response. The EMT was induced by exposure of SMMC-7721 with 0.25 mmol.L-1 of sodium nitrite, which was characterized by increased level of Vimentin, decreased E-cadherin and elevated activity of migration and metastatic potential. The results suggest that sodium nitrite could induce SMMC-7721 EMT by increased secretion of TGF-beta1 and IL-8.</abstract><cop>China</cop><pmid>21800536</pmid><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Cadherins - metabolism Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Cell Line, Tumor Cell Movement Dose-Response Relationship, Drug Epithelial-Mesenchymal Transition - drug effects Humans Interleukin-6 - secretion Interleukin-8 - secretion Liver Neoplasms - metabolism Liver Neoplasms - pathology Neoplasm Invasiveness NF-kappa B - metabolism Proto-Oncogene Proteins c-akt - metabolism Sodium Nitrite - administration & dosage Sodium Nitrite - pharmacology Transforming Growth Factor beta1 - secretion Twist-Related Protein 1 - metabolism Vimentin - metabolism |
| title | Sodium nitrite induces epithelial-mesenchymal transition of SMMC-7721 cells |
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