Systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences
► We did systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences. ► The level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. ► Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7me...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2011-07, Vol.411 (2), p.393-396 |
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| creator | Lee, Han-Chia Chen, Chih-Ying Au, Lo-Chun |
| description | ► We did systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences. ► The level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. ► Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7mer-1A. ► A mismatch in one seed dramatically decreasing repressive activity of the dimer indicating cooperation of two monomers. ► Strong repression of miR-155 and siRNA-155 (siRNA carrying miR-155 sequence) with perfectly matched target was due primarily to translational attenuation.
Most miRNA target sites are determined by Watson–Crick pairing via the seed region (positions ∼2–7nt of the mature miRNA). The binding sites of target mRNAs are categorized primarily as 7mer-m8, the 7mer-1A and the 8mer sites. This study analyzed post-transcriptional repression as a function of associations among various seed/target sequences. The target sequence of miR-155 from TP53INP1 was modified such that their various monomers and dimers could be inserted into the 3′UTR of a reporter gene for monitoring repression activity of miR-155. Results revealed that the level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. For dimeric targets, the order is 2×8mer>2×7mer-m8>2×7mer-1A. Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7mer-1A. A mismatch in one seed dramatically decreasing repressive activity of the dimer. This indicated that the degree of repression could be synergistically enhanced through the cooperation of the two miRISC-loaded monomers. The siRNA-155 (siRNA carrying miR-155 sequence) elicited higher repressive activity than miR-155, as they bound to the perfectly matched target. However, strong repression of miR-155 and siRNA-155 with a perfectly matched target was due primarily to translational attenuation. Cleavage/degradation of the target mRNA was not a major cause of the observed repression. |
| doi_str_mv | 10.1016/j.bbrc.2011.06.159 |
| format | Article |
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Most miRNA target sites are determined by Watson–Crick pairing via the seed region (positions ∼2–7nt of the mature miRNA). The binding sites of target mRNAs are categorized primarily as 7mer-m8, the 7mer-1A and the 8mer sites. This study analyzed post-transcriptional repression as a function of associations among various seed/target sequences. The target sequence of miR-155 from TP53INP1 was modified such that their various monomers and dimers could be inserted into the 3′UTR of a reporter gene for monitoring repression activity of miR-155. Results revealed that the level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. For dimeric targets, the order is 2×8mer>2×7mer-m8>2×7mer-1A. Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7mer-1A. A mismatch in one seed dramatically decreasing repressive activity of the dimer. This indicated that the degree of repression could be synergistically enhanced through the cooperation of the two miRISC-loaded monomers. The siRNA-155 (siRNA carrying miR-155 sequence) elicited higher repressive activity than miR-155, as they bound to the perfectly matched target. However, strong repression of miR-155 and siRNA-155 with a perfectly matched target was due primarily to translational attenuation. Cleavage/degradation of the target mRNA was not a major cause of the observed repression.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2011.06.159</identifier><identifier>PMID: 21749858</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Base Sequence ; Carrier Proteins - genetics ; Cell Line, Tumor ; Gene Expression Regulation ; Genes, Reporter ; Heat-Shock Proteins - genetics ; Humans ; Luciferases - genetics ; MicroRNAs - genetics ; miR-155 ; miRNA target ; Nucleic Acid Conformation ; Reporter assay ; Repressive activity ; RNA, Small Interfering - genetics ; siRNA</subject><ispartof>Biochemical and biophysical research communications, 2011-07, Vol.411 (2), p.393-396</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-aedf89740301e1944a16c6e6cb8e4b90dfe8d2f9418fe90122ebf2d7183af3433</citedby><cites>FETCH-LOGICAL-c388t-aedf89740301e1944a16c6e6cb8e4b90dfe8d2f9418fe90122ebf2d7183af3433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2011.06.159$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21749858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Han-Chia</creatorcontrib><creatorcontrib>Chen, Chih-Ying</creatorcontrib><creatorcontrib>Au, Lo-Chun</creatorcontrib><title>Systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► We did systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences. ► The level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. ► Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7mer-1A. ► A mismatch in one seed dramatically decreasing repressive activity of the dimer indicating cooperation of two monomers. ► Strong repression of miR-155 and siRNA-155 (siRNA carrying miR-155 sequence) with perfectly matched target was due primarily to translational attenuation.
Most miRNA target sites are determined by Watson–Crick pairing via the seed region (positions ∼2–7nt of the mature miRNA). The binding sites of target mRNAs are categorized primarily as 7mer-m8, the 7mer-1A and the 8mer sites. This study analyzed post-transcriptional repression as a function of associations among various seed/target sequences. The target sequence of miR-155 from TP53INP1 was modified such that their various monomers and dimers could be inserted into the 3′UTR of a reporter gene for monitoring repression activity of miR-155. Results revealed that the level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. For dimeric targets, the order is 2×8mer>2×7mer-m8>2×7mer-1A. Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7mer-1A. A mismatch in one seed dramatically decreasing repressive activity of the dimer. This indicated that the degree of repression could be synergistically enhanced through the cooperation of the two miRISC-loaded monomers. The siRNA-155 (siRNA carrying miR-155 sequence) elicited higher repressive activity than miR-155, as they bound to the perfectly matched target. However, strong repression of miR-155 and siRNA-155 with a perfectly matched target was due primarily to translational attenuation. Cleavage/degradation of the target mRNA was not a major cause of the observed repression.</description><subject>Base Sequence</subject><subject>Carrier Proteins - genetics</subject><subject>Cell Line, Tumor</subject><subject>Gene Expression Regulation</subject><subject>Genes, Reporter</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Humans</subject><subject>Luciferases - genetics</subject><subject>MicroRNAs - genetics</subject><subject>miR-155</subject><subject>miRNA target</subject><subject>Nucleic Acid Conformation</subject><subject>Reporter assay</subject><subject>Repressive activity</subject><subject>RNA, Small Interfering - genetics</subject><subject>siRNA</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo7rj6BzxIbnrptpLOdCfgZVn8gkXBD_AW0klFM0x3j6nMyvx708zqcU9VBU-9BfUw9lxAK0D0r3ftOGbfShCihb4VW_OAbQQYaKQA9ZBtAKBvpBE_LtgToh1UUPXmMbuQYlBGb_WG5a8nKjglz_0yHVxOtMx8iTzjISNRqpPzJd2mcuJxyXxKXz5dcTcHTueOaPHJFQz8Tyq_eEgxYsa58HI6IK1RxeWfWDjh7yPOHukpexTdnvDZXb1k39-9_Xb9obn5_P7j9dVN4zutS-MwRG0GBR0IFEYpJ3rfY-9HjWo0ECLqIKNRQkc0IKTEMcowCN252Kmuu2Qvz7mHvNTTVOyUyON-72ZcjmS1hkEoqaGSr-4lBVRqGKTaVlSeUZ8XoozRHnKaXD5VyK5W7M6uVuxqxUJvq5W69OIu_zhOGP6v_NNQgTdnAOs_bhNmSz6tzwopoy82LOm-_L8Wr59r</recordid><startdate>20110729</startdate><enddate>20110729</enddate><creator>Lee, Han-Chia</creator><creator>Chen, Chih-Ying</creator><creator>Au, Lo-Chun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20110729</creationdate><title>Systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences</title><author>Lee, Han-Chia ; Chen, Chih-Ying ; Au, Lo-Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-aedf89740301e1944a16c6e6cb8e4b90dfe8d2f9418fe90122ebf2d7183af3433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Base Sequence</topic><topic>Carrier Proteins - genetics</topic><topic>Cell Line, Tumor</topic><topic>Gene Expression Regulation</topic><topic>Genes, Reporter</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Humans</topic><topic>Luciferases - genetics</topic><topic>MicroRNAs - genetics</topic><topic>miR-155</topic><topic>miRNA target</topic><topic>Nucleic Acid Conformation</topic><topic>Reporter assay</topic><topic>Repressive activity</topic><topic>RNA, Small Interfering - genetics</topic><topic>siRNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Han-Chia</creatorcontrib><creatorcontrib>Chen, Chih-Ying</creatorcontrib><creatorcontrib>Au, Lo-Chun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Han-Chia</au><au>Chen, Chih-Ying</au><au>Au, Lo-Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2011-07-29</date><risdate>2011</risdate><volume>411</volume><issue>2</issue><spage>393</spage><epage>396</epage><pages>393-396</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► We did systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences. ► The level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. ► Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7mer-1A. ► A mismatch in one seed dramatically decreasing repressive activity of the dimer indicating cooperation of two monomers. ► Strong repression of miR-155 and siRNA-155 (siRNA carrying miR-155 sequence) with perfectly matched target was due primarily to translational attenuation.
Most miRNA target sites are determined by Watson–Crick pairing via the seed region (positions ∼2–7nt of the mature miRNA). The binding sites of target mRNAs are categorized primarily as 7mer-m8, the 7mer-1A and the 8mer sites. This study analyzed post-transcriptional repression as a function of associations among various seed/target sequences. The target sequence of miR-155 from TP53INP1 was modified such that their various monomers and dimers could be inserted into the 3′UTR of a reporter gene for monitoring repression activity of miR-155. Results revealed that the level of repression could be ordered as follows: perfect-matched target≫dimeric targets≫monomeric targets. For dimeric targets, the order is 2×8mer>2×7mer-m8>2×7mer-1A. Fold repression of 8mer+7mer-1A lay between 2×8mer and 2×7mer-1A. A mismatch in one seed dramatically decreasing repressive activity of the dimer. This indicated that the degree of repression could be synergistically enhanced through the cooperation of the two miRISC-loaded monomers. The siRNA-155 (siRNA carrying miR-155 sequence) elicited higher repressive activity than miR-155, as they bound to the perfectly matched target. However, strong repression of miR-155 and siRNA-155 with a perfectly matched target was due primarily to translational attenuation. Cleavage/degradation of the target mRNA was not a major cause of the observed repression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21749858</pmid><doi>10.1016/j.bbrc.2011.06.159</doi><tpages>4</tpages></addata></record> |
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| subjects | Base Sequence Carrier Proteins - genetics Cell Line, Tumor Gene Expression Regulation Genes, Reporter Heat-Shock Proteins - genetics Humans Luciferases - genetics MicroRNAs - genetics miR-155 miRNA target Nucleic Acid Conformation Reporter assay Repressive activity RNA, Small Interfering - genetics siRNA |
| title | Systemic comparison of repression activity for miRNA and siRNA associated with different types of target sequences |
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