Transfection of cells using flow-through electroporation based on constant voltage

Electroporation is a high-efficiency and low-toxicity physical gene transfer method. Classical electroporation protocols are limited by the small volume of cell samples processed (less than 10 7 cells per reaction) and low DNA uptake due to partial permeabilization of the cell membrane. Here we desc...

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Veröffentlicht in:	Nature protocols 2011-08, Vol.6 (8), p.1192-1208
Hauptverfasser:	Geng, Tao, Zhan, Yihong, Wang, Jun, Lu, Chang
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2204 631/1647/2300/1851 Analytical Chemistry Animals Biological Techniques Biomedical and Life Sciences Cell Membrane Permeability Cells, Cultured CHO Cells Computational Biology/Bioinformatics Cricetinae Cricetulus Deoxyribonucleic acid DNA DNA - chemistry DNA - metabolism Electric potential Electroporation Electroporation - instrumentation Electroporation - methods Equipment Design Fabrication Gene therapy Gene transfer Gene Transfer Techniques Genetically modified organisms Infection Life Sciences Microarrays Organic Chemistry Plasmids - genetics Power supplies protocol Toxicity Transfection Transfection - instrumentation Transfection - methods Voltage
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