Oriented Reconstitution of a Membrane Protein in a Giant Unilamellar Vesicle: Experimental Verification with the Potassium Channel KcsA

We report a method for the successful reconstitution of the KcsA potassium channel with either an outside-out or inside-out orientation in giant unilamellar vesicles, using the droplet-transfer technique. The procedure is rather simple. First, we prepared water-in-oil droplets lined with a lipid mon...

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Veröffentlicht in:	Journal of the American Chemical Society 2011-08, Vol.133 (30), p.11774-11779
Hauptverfasser:	Yanagisawa, Miho, Iwamoto, Masayuki, Kato, Ayako, Yoshikawa, Kenichi, Oiki, Shigetoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Membrane Proteins - chemistry Potassium Channels - chemistry Streptomyces lividans - chemistry Unilamellar Liposomes - chemistry
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