Improved technique for stereotactic placement of nerve grafts between two locations inside the rat brain
Peripheral nerve grafts have shown the ability to facilitate central axonal growth and regenerate the adult central nervous system. However, the detailed description of a technique for atraumatic graft placement within the brain is lacking. We present a stereotactic procedure to implant a peripheral...
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| Veröffentlicht in: | Journal of neuroscience methods 2008-09, Vol.174 (2), p.194-201 |
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| container_title | Journal of neuroscience methods |
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| creator | Gómez-Pinedo, Ulises Félez, M. Carmen Sancho-Bielsa, Francisco J. Vidueira, Sandra Cabanes, Carmen Soriano, Mario García-Verdugo, José M. Barcia, Juan A. |
| description | Peripheral nerve grafts have shown the ability to facilitate central axonal growth and regenerate the adult central nervous system. However, the detailed description of a technique for atraumatic graft placement within the brain is lacking. We present a stereotactic procedure to implant a peripheral nerve graft within a rat's brain with minimal brain tissue damage. The procedure permits a correct graft placement joining two chosen points, and the survival and integration of the graft in the host tissue with a light glial reaction, with evidence of central axonal growth inside the graft, at least up to 8 weeks after its implantation. |
| doi_str_mv | 10.1016/j.jneumeth.2008.07.008 |
| format | Article |
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| ispartof | Journal of neuroscience methods, 2008-09, Vol.174 (2), p.194-201 |
| issn | 0165-0270 1872-678X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Brain - surgery Brain - ultrastructure Grafting stereotactic cannula Microscopy, Electron, Transmission Nerve Regeneration - physiology Nigrostriatal pathway Peripheral nerve grafts Rats Regeneration Sciatic Nerve - transplantation Stereotactic placement Stereotaxic Techniques |
| title | Improved technique for stereotactic placement of nerve grafts between two locations inside the rat brain |
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