Studies on immobilizd lipase in hydrophobic sol-gel
The hydrolysis of tetraethoxysilane using the sol-gel process was used to produce silica matrices, and these were tested for the immobilization of lipase from Candida rugosa by three methods: physical adsorption, covalent binding, and gel entrapment in the presence and absence of polyethylene glycol...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2004-03, Vol.113 (1-3), p.307-319 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Soares, Cleide M F Dos Santos, Onelia A de Castro, Heizir F de Moraes, Flavio F Zanin, Gisella M |
| description | The hydrolysis of tetraethoxysilane using the sol-gel process was used to produce silica matrices, and these were tested for the immobilization of lipase from Candida rugosa by three methods: physical adsorption, covalent binding, and gel entrapment in the presence and absence of polyethylene glycol (PEG-1450). The silica matrices and their derivatives were characterized regarding particle size distribution, specific surface area, pore size distribution (Brunauer, Emmett, and Teller [B.E.T.] method), yield of grafting (thermogravimetric analyzer [TGA]), and chemical composition (Fourier transform infrared). Immobilization yields based on recovered lipase activity varied from 3.0 to 32.0%, and the highest efficiency was attained when lipase was encapsulated in the presence of PEG. |
| doi_str_mv | 10.1385/ABAB:113:1-3:307 |
| format | Article |
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The silica matrices and their derivatives were characterized regarding particle size distribution, specific surface area, pore size distribution (Brunauer, Emmett, and Teller [B.E.T.] method), yield of grafting (thermogravimetric analyzer [TGA]), and chemical composition (Fourier transform infrared). 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The silica matrices and their derivatives were characterized regarding particle size distribution, specific surface area, pore size distribution (Brunauer, Emmett, and Teller [B.E.T.] method), yield of grafting (thermogravimetric analyzer [TGA]), and chemical composition (Fourier transform infrared). 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| subjects | Biochemistry Candida rugosa Chemical composition Entrapment Enzymes Fourier transforms Fungi Hydrophobicity Immobilization Infrared analysis Lipase Particle size distribution Polyethylene glycol Pore size Pore size distribution Silica Silicon dioxide Size distribution Sol-gel processes Studies Tetraethoxysilane Tetraethyl orthosilicate |
| title | Studies on immobilizd lipase in hydrophobic sol-gel |
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