In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells
In South America, visceral leishmaniasis is a zoonosis caused by the protozoan species Leishmania infantum (syn. L. chagasi) and is primarily transmitted through the bite of the female Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommende...
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| Veröffentlicht in: | Veterinary parasitology 2011-06, Vol.178 (3), p.235-240 |
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| creator | Rondon, Fernanda C.M. Bevilaqua, Claudia M.L. Accioly, Marina P. Morais, Selene M. Andrade-Junior, Heitor F. Machado, Lyeghyna K.A. Cardoso, Roselaine P.A. Almeida, Camila A. Queiroz-Junior, Eudson M. Rodrigues, Ana Caroline M. |
| description | In South America, visceral leishmaniasis is a zoonosis caused by the protozoan species
Leishmania infantum (syn.
L. chagasi) and is primarily transmitted through the bite of the female
Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommended by health agencies have not achieved significant results in reducing the incidence of human cases, and the lack of effective drugs to treat dogs resulted in the prohibition of this course of action in Brazil. Therefore, it is necessary to search new alternatives for the treatment of canine and human visceral leishmaniasis. The objectives of this study were to evaluate the
in vitro effect of fractions from
Aloe vera (aloe),
Coriandrum sativum (coriander), and
Ricinus communis (castor) on promastigotes and amastigotes of
L. infantum and to analyze the toxicity against the murine monocytic cells RAW 264.7. To determine the viability of these substances on 50% parasites (IC50), we used a tetrazolium dye (MTT) colorimetric assay (bromide 3-4.5-dimethylthiazol-2-yl-2,5-dephenyltetrazolium), and on amastigotes we performed an
in situ ELISA. All fractions were effective against
L. infantum promastigotes and did not differ from the positive control pentamidine (
p
>
0.05). However, the
R. communis ethyl acetate and chloroform fractions, as well as the
C. sativum methanol fraction, were the most effective against amastigotes and did not differ from the positive control amphotericin B (
p
>
0.05). The
R. communis ethyl acetate fraction was the least toxic, presenting 83.5% viability of RAW 264.7 cells, which was similar to the results obtained with amphotericin B (
p
>
0.05). Based on these results, we intend to undertake
in vivo studies with
R. communis ethyl acetate fractions due the high effectiveness against amastigotes and promastigotes of
L. infantum and the low cytotoxicity towards murine monocytic cells. |
| doi_str_mv | 10.1016/j.vetpar.2011.01.007 |
| format | Article |
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Leishmania infantum (syn.
L. chagasi) and is primarily transmitted through the bite of the female
Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommended by health agencies have not achieved significant results in reducing the incidence of human cases, and the lack of effective drugs to treat dogs resulted in the prohibition of this course of action in Brazil. Therefore, it is necessary to search new alternatives for the treatment of canine and human visceral leishmaniasis. The objectives of this study were to evaluate the
in vitro effect of fractions from
Aloe vera (aloe),
Coriandrum sativum (coriander), and
Ricinus communis (castor) on promastigotes and amastigotes of
L. infantum and to analyze the toxicity against the murine monocytic cells RAW 264.7. To determine the viability of these substances on 50% parasites (IC50), we used a tetrazolium dye (MTT) colorimetric assay (bromide 3-4.5-dimethylthiazol-2-yl-2,5-dephenyltetrazolium), and on amastigotes we performed an
in situ ELISA. All fractions were effective against
L. infantum promastigotes and did not differ from the positive control pentamidine (
p
>
0.05). However, the
R. communis ethyl acetate and chloroform fractions, as well as the
C. sativum methanol fraction, were the most effective against amastigotes and did not differ from the positive control amphotericin B (
p
>
0.05). The
R. communis ethyl acetate fraction was the least toxic, presenting 83.5% viability of RAW 264.7 cells, which was similar to the results obtained with amphotericin B (
p
>
0.05). Based on these results, we intend to undertake
in vivo studies with
R. communis ethyl acetate fractions due the high effectiveness against amastigotes and promastigotes of
L. infantum and the low cytotoxicity towards murine monocytic cells.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2011.01.007</identifier><identifier>PMID: 21320755</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aloe ; Aloe - chemistry ; Aloe vera ; Amastigotes ; Animals ; Cell Line ; Cell Survival - drug effects ; Coriandrum - chemistry ; Coriandrum sativum ; Cytotoxicity ; Inhibitory Concentration 50 ; Leishmania infantum ; Leishmania infantum - drug effects ; Leishmania infantum - growth & development ; Leishmaniasis, Visceral - drug therapy ; Leishmanicidal activity ; Lutzomyia longipalpis ; Mice ; Monocytes - drug effects ; Plant Extracts - pharmacology ; Promastigotes ; Regression Analysis ; Ricinus - chemistry ; Ricinus communis</subject><ispartof>Veterinary parasitology, 2011-06, Vol.178 (3), p.235-240</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-680add392086317a65179a137a6aa99a204a323eda4e6ff68c415cf1aedfcaac3</citedby><cites>FETCH-LOGICAL-c393t-680add392086317a65179a137a6aa99a204a323eda4e6ff68c415cf1aedfcaac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401711000409$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21320755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rondon, Fernanda C.M.</creatorcontrib><creatorcontrib>Bevilaqua, Claudia M.L.</creatorcontrib><creatorcontrib>Accioly, Marina P.</creatorcontrib><creatorcontrib>Morais, Selene M.</creatorcontrib><creatorcontrib>Andrade-Junior, Heitor F.</creatorcontrib><creatorcontrib>Machado, Lyeghyna K.A.</creatorcontrib><creatorcontrib>Cardoso, Roselaine P.A.</creatorcontrib><creatorcontrib>Almeida, Camila A.</creatorcontrib><creatorcontrib>Queiroz-Junior, Eudson M.</creatorcontrib><creatorcontrib>Rodrigues, Ana Caroline M.</creatorcontrib><title>In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>In South America, visceral leishmaniasis is a zoonosis caused by the protozoan species
Leishmania infantum (syn.
L. chagasi) and is primarily transmitted through the bite of the female
Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommended by health agencies have not achieved significant results in reducing the incidence of human cases, and the lack of effective drugs to treat dogs resulted in the prohibition of this course of action in Brazil. Therefore, it is necessary to search new alternatives for the treatment of canine and human visceral leishmaniasis. The objectives of this study were to evaluate the
in vitro effect of fractions from
Aloe vera (aloe),
Coriandrum sativum (coriander), and
Ricinus communis (castor) on promastigotes and amastigotes of
L. infantum and to analyze the toxicity against the murine monocytic cells RAW 264.7. To determine the viability of these substances on 50% parasites (IC50), we used a tetrazolium dye (MTT) colorimetric assay (bromide 3-4.5-dimethylthiazol-2-yl-2,5-dephenyltetrazolium), and on amastigotes we performed an
in situ ELISA. All fractions were effective against
L. infantum promastigotes and did not differ from the positive control pentamidine (
p
>
0.05). However, the
R. communis ethyl acetate and chloroform fractions, as well as the
C. sativum methanol fraction, were the most effective against amastigotes and did not differ from the positive control amphotericin B (
p
>
0.05). The
R. communis ethyl acetate fraction was the least toxic, presenting 83.5% viability of RAW 264.7 cells, which was similar to the results obtained with amphotericin B (
p
>
0.05). Based on these results, we intend to undertake
in vivo studies with
R. communis ethyl acetate fractions due the high effectiveness against amastigotes and promastigotes of
L. infantum and the low cytotoxicity towards murine monocytic cells.</description><subject>Aloe</subject><subject>Aloe - chemistry</subject><subject>Aloe vera</subject><subject>Amastigotes</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Coriandrum - chemistry</subject><subject>Coriandrum sativum</subject><subject>Cytotoxicity</subject><subject>Inhibitory Concentration 50</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - drug effects</subject><subject>Leishmania infantum - growth & development</subject><subject>Leishmaniasis, Visceral - drug therapy</subject><subject>Leishmanicidal activity</subject><subject>Lutzomyia longipalpis</subject><subject>Mice</subject><subject>Monocytes - drug effects</subject><subject>Plant Extracts - pharmacology</subject><subject>Promastigotes</subject><subject>Regression Analysis</subject><subject>Ricinus - chemistry</subject><subject>Ricinus communis</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE-LFDEQxYMo7rj6DURy82KPlU53p_siLIN_FgYE0XMo0xXMMEnGJN2w9_3gZpjRo1BQKfJeveTH2GsBWwFieH_YrlROmLYtCLGFWqCesI0YlWzavoenbAMSuqYDoW7Yi5wPANDBoJ6zm1bIFlTfb9jjfeCrKylyspZM4dHyu2MkvlLCd3wXk8Mwp8XzjMWttdeRf3PGhSVzE71fgsvcJjTFxZB5DHxPLv_yGBxyFyyGcnXVK78kF4j7GKJ5KM5wQ8djfsmeWTxmenXtt-zHp4_fd1-a_dfP97u7fWPkJEszjIDzLKcWxkEKhUMv1IRC1hPiNGELHcpW0owdDdYOo-lEb6xAmq1BNPKWvb3sPaX4e6FctHf5_AIMFJesRzV1qhe9qMruojQp5pzI6lNyHtODFqDP-PVBX_DrM34NtUBV25trwPLT0_zP9Jd3FXy4CKh-c3WUdDaOgqHZpUpfz9H9P-EPmVaazw</recordid><startdate>20110610</startdate><enddate>20110610</enddate><creator>Rondon, Fernanda C.M.</creator><creator>Bevilaqua, Claudia M.L.</creator><creator>Accioly, Marina P.</creator><creator>Morais, Selene M.</creator><creator>Andrade-Junior, Heitor F.</creator><creator>Machado, Lyeghyna K.A.</creator><creator>Cardoso, Roselaine P.A.</creator><creator>Almeida, Camila A.</creator><creator>Queiroz-Junior, Eudson M.</creator><creator>Rodrigues, Ana Caroline M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20110610</creationdate><title>In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells</title><author>Rondon, Fernanda C.M. ; Bevilaqua, Claudia M.L. ; Accioly, Marina P. ; Morais, Selene M. ; Andrade-Junior, Heitor F. ; Machado, Lyeghyna K.A. ; Cardoso, Roselaine P.A. ; Almeida, Camila A. ; Queiroz-Junior, Eudson M. ; Rodrigues, Ana Caroline M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-680add392086317a65179a137a6aa99a204a323eda4e6ff68c415cf1aedfcaac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aloe</topic><topic>Aloe - chemistry</topic><topic>Aloe vera</topic><topic>Amastigotes</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Coriandrum - chemistry</topic><topic>Coriandrum sativum</topic><topic>Cytotoxicity</topic><topic>Inhibitory Concentration 50</topic><topic>Leishmania infantum</topic><topic>Leishmania infantum - drug effects</topic><topic>Leishmania infantum - growth & development</topic><topic>Leishmaniasis, Visceral - drug therapy</topic><topic>Leishmanicidal activity</topic><topic>Lutzomyia longipalpis</topic><topic>Mice</topic><topic>Monocytes - drug effects</topic><topic>Plant Extracts - pharmacology</topic><topic>Promastigotes</topic><topic>Regression Analysis</topic><topic>Ricinus - chemistry</topic><topic>Ricinus communis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rondon, Fernanda C.M.</creatorcontrib><creatorcontrib>Bevilaqua, Claudia M.L.</creatorcontrib><creatorcontrib>Accioly, Marina P.</creatorcontrib><creatorcontrib>Morais, Selene M.</creatorcontrib><creatorcontrib>Andrade-Junior, Heitor F.</creatorcontrib><creatorcontrib>Machado, Lyeghyna K.A.</creatorcontrib><creatorcontrib>Cardoso, Roselaine P.A.</creatorcontrib><creatorcontrib>Almeida, Camila A.</creatorcontrib><creatorcontrib>Queiroz-Junior, Eudson M.</creatorcontrib><creatorcontrib>Rodrigues, Ana Caroline M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rondon, Fernanda C.M.</au><au>Bevilaqua, Claudia M.L.</au><au>Accioly, Marina P.</au><au>Morais, Selene M.</au><au>Andrade-Junior, Heitor F.</au><au>Machado, Lyeghyna K.A.</au><au>Cardoso, Roselaine P.A.</au><au>Almeida, Camila A.</au><au>Queiroz-Junior, Eudson M.</au><au>Rodrigues, Ana Caroline M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2011-06-10</date><risdate>2011</risdate><volume>178</volume><issue>3</issue><spage>235</spage><epage>240</epage><pages>235-240</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>In South America, visceral leishmaniasis is a zoonosis caused by the protozoan species
Leishmania infantum (syn.
L. chagasi) and is primarily transmitted through the bite of the female
Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommended by health agencies have not achieved significant results in reducing the incidence of human cases, and the lack of effective drugs to treat dogs resulted in the prohibition of this course of action in Brazil. Therefore, it is necessary to search new alternatives for the treatment of canine and human visceral leishmaniasis. The objectives of this study were to evaluate the
in vitro effect of fractions from
Aloe vera (aloe),
Coriandrum sativum (coriander), and
Ricinus communis (castor) on promastigotes and amastigotes of
L. infantum and to analyze the toxicity against the murine monocytic cells RAW 264.7. To determine the viability of these substances on 50% parasites (IC50), we used a tetrazolium dye (MTT) colorimetric assay (bromide 3-4.5-dimethylthiazol-2-yl-2,5-dephenyltetrazolium), and on amastigotes we performed an
in situ ELISA. All fractions were effective against
L. infantum promastigotes and did not differ from the positive control pentamidine (
p
>
0.05). However, the
R. communis ethyl acetate and chloroform fractions, as well as the
C. sativum methanol fraction, were the most effective against amastigotes and did not differ from the positive control amphotericin B (
p
>
0.05). The
R. communis ethyl acetate fraction was the least toxic, presenting 83.5% viability of RAW 264.7 cells, which was similar to the results obtained with amphotericin B (
p
>
0.05). Based on these results, we intend to undertake
in vivo studies with
R. communis ethyl acetate fractions due the high effectiveness against amastigotes and promastigotes of
L. infantum and the low cytotoxicity towards murine monocytic cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21320755</pmid><doi>10.1016/j.vetpar.2011.01.007</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2011-06, Vol.178 (3), p.235-240 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_879475151 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aloe Aloe - chemistry Aloe vera Amastigotes Animals Cell Line Cell Survival - drug effects Coriandrum - chemistry Coriandrum sativum Cytotoxicity Inhibitory Concentration 50 Leishmania infantum Leishmania infantum - drug effects Leishmania infantum - growth & development Leishmaniasis, Visceral - drug therapy Leishmanicidal activity Lutzomyia longipalpis Mice Monocytes - drug effects Plant Extracts - pharmacology Promastigotes Regression Analysis Ricinus - chemistry Ricinus communis |
| title | In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells |
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