Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G.hirsutum L. by SSR and GISH analyses
With 2 figures and 3 tables Gossypium australe F. Muell (2n=2x=26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n=4x=52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. austra...
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| Veröffentlicht in: | Plant breeding 2011-02, Vol.130 (1), p.60-66 |
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| creator | Sarr, D Lacape, J-M Rodier-Goud, M Jacquemin, J-M Benbouza, H Toussaint, A Palm, R Ahoton, L Baudoin, J-P Mergeai, G |
| description | With 2 figures and 3 tables Gossypium australe F. Muell (2n=2x=26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n=4x=52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. australe in G. hirsutum, the [2(G. hirsutumG. australe) G. hirsutum] pentaploid (2n=5x=65) was backcrossed as male parent to G. hirsutum. Analysis of 42 BC1 plants and seven alien addition lines, already available, with 150 SSR markers developed from G. hirsutum revealed a cross-species amplification rate of 100% and a polymorphism rate of 56%. Eighty polymorphic SSR markers generated 87G. australe-specific loci that have been assigned by a hierarchical cluster analysis to 13 linkage groups corresponding to the 13 chromosomes of G. australe. Analysis by SSR markers and genomic in situ hybridization of the self-progeny of disomic alien addition lines, backcross progeny of the pentaploid, allowed the isolation of five new MAALs. |
| doi_str_mv | 10.1111/j.1439-0523.2010.01819.x |
| format | Article |
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| ispartof | Plant breeding, 2011-02, Vol.130 (1), p.60-66 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Genetic crosses Genomic in situ hybridization Gossypium Linkage analysis Plant breeding |
| title | Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G.hirsutum L. by SSR and GISH analyses |
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