Short wavelength fundus autofluorescence versus near-infrared fundus autofluorescence, with microperimetric correspondence, in patients with geographic atrophy due to age-related macular degeneration

AimTo compare standard short-wavelength fundus autofluorescence (SW-FAF) and near infrared-wavelength fundus autofluorescence (NIR-FAF) in detecting geographic atrophy (GA) secondary to age-related macular degeneration, and its retinal sensitivity impairment.MethodsTwenty-five consecutive patients (...

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Veröffentlicht in:British journal of ophthalmology 2011-08, Vol.95 (8), p.1140-1144
Hauptverfasser: Pilotto, Elisabetta, Vujosevic, Stela, Melis, Riccardo, Convento, Enrica, Sportiello, Patrik, Alemany-Rubio, Ernesto, Segalina, Sara, Midena, Edoardo
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container_end_page 1144
container_issue 8
container_start_page 1140
container_title British journal of ophthalmology
container_volume 95
creator Pilotto, Elisabetta
Vujosevic, Stela
Melis, Riccardo
Convento, Enrica
Sportiello, Patrik
Alemany-Rubio, Ernesto
Segalina, Sara
Midena, Edoardo
description AimTo compare standard short-wavelength fundus autofluorescence (SW-FAF) and near infrared-wavelength fundus autofluorescence (NIR-FAF) in detecting geographic atrophy (GA) secondary to age-related macular degeneration, and its retinal sensitivity impairment.MethodsTwenty-five consecutive patients (36 eyes) affected by GA were studied by means of fundus autofluorescence imaging, using both SW-FAF (excitation: 488 nm, emission >500 nm) and NIR-FAF (excitation: 787 nm, emission >800 nm). All patients underwent microperimetry to assess fixation characteristics and retinal sensitivity.ResultsIn the extrafoveal region, the total hypoautofluorescent (hypo-FAF) area was significantly wider with NIR-FAF than with SW-FAF (8.03±6.68 mm2 vs 7.37±6.34 mm2 respectively; p=0.005). In the foveal area, the total hypo-FAF area was smaller with NIR-FAF than with SW-FAF (0.19±0.03 mm2 versus 0.42±0.12 mm2 respectively; p=0.008). Foveal sparing was larger at NIR-FAF compared with SW-FAF (p=0.021). In nine cases (25%) the site of fixation was hypoautofluorescent on SW-FAF, but normal on NIR-FAF with preserved retinal sensitivity.ConclusionsStandard SW-FAF may overestimate GA in the foveal area, correctly detected by NIR-FAF. In the extrafoveal area, SW-FAF may underestimate GA. Standard SW-FAF should be integrated with NIR FAF when detecting and following GA to avoid inconsistent results and misinterpretation, from both a morphological and functional perspective. Microperimetry helps to quantify retinal sensitivity in GA.
doi_str_mv 10.1136/bjo.2010.187344
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All patients underwent microperimetry to assess fixation characteristics and retinal sensitivity.ResultsIn the extrafoveal region, the total hypoautofluorescent (hypo-FAF) area was significantly wider with NIR-FAF than with SW-FAF (8.03±6.68 mm2 vs 7.37±6.34 mm2 respectively; p=0.005). In the foveal area, the total hypo-FAF area was smaller with NIR-FAF than with SW-FAF (0.19±0.03 mm2 versus 0.42±0.12 mm2 respectively; p=0.008). Foveal sparing was larger at NIR-FAF compared with SW-FAF (p=0.021). In nine cases (25%) the site of fixation was hypoautofluorescent on SW-FAF, but normal on NIR-FAF with preserved retinal sensitivity.ConclusionsStandard SW-FAF may overestimate GA in the foveal area, correctly detected by NIR-FAF. In the extrafoveal area, SW-FAF may underestimate GA. Standard SW-FAF should be integrated with NIR FAF when detecting and following GA to avoid inconsistent results and misinterpretation, from both a morphological and functional perspective. Microperimetry helps to quantify retinal sensitivity in GA.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.2010.187344</identifier><identifier>PMID: 20974627</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>age-related macular degeneration ; Aged ; Aged, 80 and over ; Biological and medical sciences ; degeneration ; Diabetes ; Diabetic retinopathy ; diagnostic tests/investigation ; Female ; Fluorescence ; Fovea Centralis - metabolism ; Fovea Centralis - pathology ; fundus autofluorescence ; Geographic atrophy ; Geographic Atrophy - etiology ; Geographic Atrophy - metabolism ; Geographic Atrophy - pathology ; Humans ; imaging ; Infrared Rays ; macula ; Macular degeneration ; Macular Degeneration - complications ; Macular Degeneration - metabolism ; Macular Degeneration - pathology ; Male ; Medical sciences ; microperimetry ; Middle Aged ; Miscellaneous ; Ophthalmology ; Ophthalmoscopy - methods ; Ophthalmoscopy - standards ; Reproducibility of Results ; Retinopathies ; Sensory Thresholds ; Software ; vision ; Visual Acuity ; Visual Field Tests - methods ; Visual Field Tests - standards ; Visual Fields</subject><ispartof>British journal of ophthalmology, 2011-08, Vol.95 (8), p.1140-1144</ispartof><rights>2011, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright: 2011 (c) 2011, Published by the BMJ Publishing Group Limited. 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All patients underwent microperimetry to assess fixation characteristics and retinal sensitivity.ResultsIn the extrafoveal region, the total hypoautofluorescent (hypo-FAF) area was significantly wider with NIR-FAF than with SW-FAF (8.03±6.68 mm2 vs 7.37±6.34 mm2 respectively; p=0.005). In the foveal area, the total hypo-FAF area was smaller with NIR-FAF than with SW-FAF (0.19±0.03 mm2 versus 0.42±0.12 mm2 respectively; p=0.008). Foveal sparing was larger at NIR-FAF compared with SW-FAF (p=0.021). In nine cases (25%) the site of fixation was hypoautofluorescent on SW-FAF, but normal on NIR-FAF with preserved retinal sensitivity.ConclusionsStandard SW-FAF may overestimate GA in the foveal area, correctly detected by NIR-FAF. In the extrafoveal area, SW-FAF may underestimate GA. Standard SW-FAF should be integrated with NIR FAF when detecting and following GA to avoid inconsistent results and misinterpretation, from both a morphological and functional perspective. Microperimetry helps to quantify retinal sensitivity in GA.</description><subject>age-related macular degeneration</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>degeneration</subject><subject>Diabetes</subject><subject>Diabetic retinopathy</subject><subject>diagnostic tests/investigation</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Fovea Centralis - metabolism</subject><subject>Fovea Centralis - pathology</subject><subject>fundus autofluorescence</subject><subject>Geographic atrophy</subject><subject>Geographic Atrophy - etiology</subject><subject>Geographic Atrophy - metabolism</subject><subject>Geographic Atrophy - pathology</subject><subject>Humans</subject><subject>imaging</subject><subject>Infrared Rays</subject><subject>macula</subject><subject>Macular degeneration</subject><subject>Macular Degeneration - complications</subject><subject>Macular Degeneration - metabolism</subject><subject>Macular Degeneration - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>microperimetry</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Ophthalmology</subject><subject>Ophthalmoscopy - methods</subject><subject>Ophthalmoscopy - standards</subject><subject>Reproducibility of Results</subject><subject>Retinopathies</subject><subject>Sensory Thresholds</subject><subject>Software</subject><subject>vision</subject><subject>Visual Acuity</subject><subject>Visual Field Tests - methods</subject><subject>Visual Field Tests - standards</subject><subject>Visual Fields</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0V2L1DAUBuAiijuuXnsnARFB7G6Spkl7qYPuCosK6noZ0vZ0pmOb1Hzsur_Qv-UZOq4giFch6XNOT_Jm2WNGTxgr5Gmzcyec7neVKoS4k62YkFXOqarvZitKqcoZk-woexDCDrdcMnU_O-K0VkJytcp-fto6H8m1uYIR7CZuSZ9slwIxKbp-TM5DaMG2QK7ABzy3YHw-2N4bD92_8EtyPWCraWi9m8EPE0Q_tKR1HsXsbLegwZLZxAFsDEvBBtzGm3mL1kQs3d6QLgGJjpgN5B5GE_Gnk2nTaDzpYAMWPHZw9mF2rzdjgEeH9Tj78vbN5_V5fvHh7N361UXeCK5irqBpmYSaglAlb4zARfRVU5QVF7zqVVOJXhVSMk6Lri-ELFXHGwaUCyGKrjjOni99Z---JwhRTwPeeRyNBZeCrlTFleJ1jfLpX3Lnkrc4nGZKVbWQopaoTheFLxWCh17P-FzG32hG9T5ijRHrfcR6iRgrnhz6pmaC7tb_zhTBswMwoTUjJmXbIfxxokBV7gfMFzeECD9uvxv_TUtVqFK_v1zrj5ev6_Ozr6Uu0b9YfDPt_jvlL--x0FQ</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Pilotto, Elisabetta</creator><creator>Vujosevic, Stela</creator><creator>Melis, Riccardo</creator><creator>Convento, Enrica</creator><creator>Sportiello, Patrik</creator><creator>Alemany-Rubio, Ernesto</creator><creator>Segalina, Sara</creator><creator>Midena, Edoardo</creator><general>BMJ Publishing Group Ltd</general><general>BMJ Publishing Group</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20110801</creationdate><title>Short wavelength fundus autofluorescence versus near-infrared fundus autofluorescence, with microperimetric correspondence, in patients with geographic atrophy due to age-related macular degeneration</title><author>Pilotto, Elisabetta ; Vujosevic, Stela ; Melis, Riccardo ; Convento, Enrica ; Sportiello, Patrik ; Alemany-Rubio, Ernesto ; Segalina, Sara ; Midena, Edoardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b427t-7ebc16e90e4752ba44754f8b3582428f7b84f73661203df34657d2b1e024443d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>age-related macular degeneration</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>degeneration</topic><topic>Diabetes</topic><topic>Diabetic retinopathy</topic><topic>diagnostic tests/investigation</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Fovea Centralis - metabolism</topic><topic>Fovea Centralis - pathology</topic><topic>fundus autofluorescence</topic><topic>Geographic atrophy</topic><topic>Geographic Atrophy - etiology</topic><topic>Geographic Atrophy - metabolism</topic><topic>Geographic Atrophy - pathology</topic><topic>Humans</topic><topic>imaging</topic><topic>Infrared Rays</topic><topic>macula</topic><topic>Macular degeneration</topic><topic>Macular Degeneration - complications</topic><topic>Macular Degeneration - metabolism</topic><topic>Macular Degeneration - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>microperimetry</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Ophthalmology</topic><topic>Ophthalmoscopy - methods</topic><topic>Ophthalmoscopy - standards</topic><topic>Reproducibility of Results</topic><topic>Retinopathies</topic><topic>Sensory Thresholds</topic><topic>Software</topic><topic>vision</topic><topic>Visual Acuity</topic><topic>Visual Field Tests - methods</topic><topic>Visual Field Tests - standards</topic><topic>Visual Fields</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pilotto, Elisabetta</creatorcontrib><creatorcontrib>Vujosevic, Stela</creatorcontrib><creatorcontrib>Melis, Riccardo</creatorcontrib><creatorcontrib>Convento, Enrica</creatorcontrib><creatorcontrib>Sportiello, Patrik</creatorcontrib><creatorcontrib>Alemany-Rubio, Ernesto</creatorcontrib><creatorcontrib>Segalina, Sara</creatorcontrib><creatorcontrib>Midena, Edoardo</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pilotto, Elisabetta</au><au>Vujosevic, Stela</au><au>Melis, Riccardo</au><au>Convento, Enrica</au><au>Sportiello, Patrik</au><au>Alemany-Rubio, Ernesto</au><au>Segalina, Sara</au><au>Midena, Edoardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short wavelength fundus autofluorescence versus near-infrared fundus autofluorescence, with microperimetric correspondence, in patients with geographic atrophy due to age-related macular degeneration</atitle><jtitle>British journal of ophthalmology</jtitle><addtitle>Br J Ophthalmol</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>95</volume><issue>8</issue><spage>1140</spage><epage>1144</epage><pages>1140-1144</pages><issn>0007-1161</issn><eissn>1468-2079</eissn><coden>BJOPAL</coden><abstract>AimTo compare standard short-wavelength fundus autofluorescence (SW-FAF) and near infrared-wavelength fundus autofluorescence (NIR-FAF) in detecting geographic atrophy (GA) secondary to age-related macular degeneration, and its retinal sensitivity impairment.MethodsTwenty-five consecutive patients (36 eyes) affected by GA were studied by means of fundus autofluorescence imaging, using both SW-FAF (excitation: 488 nm, emission &gt;500 nm) and NIR-FAF (excitation: 787 nm, emission &gt;800 nm). All patients underwent microperimetry to assess fixation characteristics and retinal sensitivity.ResultsIn the extrafoveal region, the total hypoautofluorescent (hypo-FAF) area was significantly wider with NIR-FAF than with SW-FAF (8.03±6.68 mm2 vs 7.37±6.34 mm2 respectively; p=0.005). In the foveal area, the total hypo-FAF area was smaller with NIR-FAF than with SW-FAF (0.19±0.03 mm2 versus 0.42±0.12 mm2 respectively; p=0.008). Foveal sparing was larger at NIR-FAF compared with SW-FAF (p=0.021). In nine cases (25%) the site of fixation was hypoautofluorescent on SW-FAF, but normal on NIR-FAF with preserved retinal sensitivity.ConclusionsStandard SW-FAF may overestimate GA in the foveal area, correctly detected by NIR-FAF. In the extrafoveal area, SW-FAF may underestimate GA. Standard SW-FAF should be integrated with NIR FAF when detecting and following GA to avoid inconsistent results and misinterpretation, from both a morphological and functional perspective. Microperimetry helps to quantify retinal sensitivity in GA.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>20974627</pmid><doi>10.1136/bjo.2010.187344</doi><tpages>5</tpages></addata></record>
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subjects age-related macular degeneration
Aged
Aged, 80 and over
Biological and medical sciences
degeneration
Diabetes
Diabetic retinopathy
diagnostic tests/investigation
Female
Fluorescence
Fovea Centralis - metabolism
Fovea Centralis - pathology
fundus autofluorescence
Geographic atrophy
Geographic Atrophy - etiology
Geographic Atrophy - metabolism
Geographic Atrophy - pathology
Humans
imaging
Infrared Rays
macula
Macular degeneration
Macular Degeneration - complications
Macular Degeneration - metabolism
Macular Degeneration - pathology
Male
Medical sciences
microperimetry
Middle Aged
Miscellaneous
Ophthalmology
Ophthalmoscopy - methods
Ophthalmoscopy - standards
Reproducibility of Results
Retinopathies
Sensory Thresholds
Software
vision
Visual Acuity
Visual Field Tests - methods
Visual Field Tests - standards
Visual Fields
title Short wavelength fundus autofluorescence versus near-infrared fundus autofluorescence, with microperimetric correspondence, in patients with geographic atrophy due to age-related macular degeneration
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