Method for monitoring of the protein amino group changes during carbonylation
Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. The method is based on the reac...
Gespeichert in:
| Veröffentlicht in: | Clinical biochemistry 2011-08, Vol.44 (12), p.994-999 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 999 |
|---|---|
| container_issue | 12 |
| container_start_page | 994 |
| container_title | Clinical biochemistry |
| container_volume | 44 |
| creator | ACIMOVIC, Jelena M JOVANOVIC, Vesna B VESELINOVIC, Milica R SRECKOVIC, Vesna Dimitrijevic MANDIC, Ljuba M |
| description | Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation.
The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation
in vitro with methylglyoxal and
in vivo in 13 patients with type 2 diabetes and 20 healthy persons.
The method is simple, fast, precise (RSD in the range of 1.2–1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p
<
0.01) in comparison with a control group.
The method developed is suitable for quantification of protein amino groups during
in vitro carbonylation as well as for clinical practice. |
| doi_str_mv | 10.1016/j.clinbiochem.2011.05.019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_878030503</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009912011003705</els_id><sourcerecordid>878030503</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-40608e24c7bfe617c18a54ee283483f55a4151115c38e6479cf9a6d3c6584933</originalsourceid><addsrcrecordid>eNqNkEuP0zAQgC0EYsvCX0DmgDgljBPbcY6o4iXtisveLXcyaV0ldrETpP33uLQ8jlxmNPI345mPsTcCagFCvz_WOPmw8xEPNNcNCFGDqkH0T9hGmK6tmr5tn7INAPRVLxq4YS9yPpaykUY_ZzeN0LK8wYbd39NyiAMfY-JzDH6JyYc9jyNfDsRPKS7kA3ezD5HvU1xPHA8u7CnzYf1Foku7GB4nt_gYXrJno5syvbrmW_bw6ePD9kt19-3z1-2Huwol6KUqAQw1ErvdSFp0KIxTkqgxrTTtqJSTQgkhFLaGtOx6HHunhxa1MrKcdsveXcaW_b6vlBc7-4w0TS5QXLM1nYEWFJzJ_kJiijknGu0p-dmlRyvAnl3ao_3HpT27tKBscVl6X19_WXczDX86f8srwNsr4DK6aUwuoM9_OSnLDbor3PbCUVHyw1OyGT0FpMEnwsUO0f_HOj8BxRKXsA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>878030503</pqid></control><display><type>article</type><title>Method for monitoring of the protein amino group changes during carbonylation</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>ACIMOVIC, Jelena M ; JOVANOVIC, Vesna B ; VESELINOVIC, Milica R ; SRECKOVIC, Vesna Dimitrijevic ; MANDIC, Ljuba M</creator><creatorcontrib>ACIMOVIC, Jelena M ; JOVANOVIC, Vesna B ; VESELINOVIC, Milica R ; SRECKOVIC, Vesna Dimitrijevic ; MANDIC, Ljuba M</creatorcontrib><description>Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation.
The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation
in vitro with methylglyoxal and
in vivo in 13 patients with type 2 diabetes and 20 healthy persons.
The method is simple, fast, precise (RSD in the range of 1.2–1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p
<
0.01) in comparison with a control group.
The method developed is suitable for quantification of protein amino groups during
in vitro carbonylation as well as for clinical practice.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2011.05.019</identifier><identifier>PMID: 21640090</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Benzoquinones - chemistry ; Biological and medical sciences ; Carbonylation ; Case-Control Studies ; Diabetes ; Diabetes Mellitus, Type 2 - metabolism ; Diabetes. Impaired glucose tolerance ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Female ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Methylglyoxal ; Protein amino group ; Protein Carbonylation ; Proteins - analysis ; Proteins - chemistry ; Proteins - metabolism ; Pyruvaldehyde - chemistry ; Pyruvaldehyde - metabolism ; Serum Albumin - analysis ; Serum Albumin - chemistry ; Serum Albumin - metabolism ; Spectrophotometric determination with p-benzoquinone ; Spectrophotometry</subject><ispartof>Clinical biochemistry, 2011-08, Vol.44 (12), p.994-999</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-40608e24c7bfe617c18a54ee283483f55a4151115c38e6479cf9a6d3c6584933</citedby><cites>FETCH-LOGICAL-c406t-40608e24c7bfe617c18a54ee283483f55a4151115c38e6479cf9a6d3c6584933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0009912011003705$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24411167$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21640090$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ACIMOVIC, Jelena M</creatorcontrib><creatorcontrib>JOVANOVIC, Vesna B</creatorcontrib><creatorcontrib>VESELINOVIC, Milica R</creatorcontrib><creatorcontrib>SRECKOVIC, Vesna Dimitrijevic</creatorcontrib><creatorcontrib>MANDIC, Ljuba M</creatorcontrib><title>Method for monitoring of the protein amino group changes during carbonylation</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation.
The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation
in vitro with methylglyoxal and
in vivo in 13 patients with type 2 diabetes and 20 healthy persons.
The method is simple, fast, precise (RSD in the range of 1.2–1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p
<
0.01) in comparison with a control group.
The method developed is suitable for quantification of protein amino groups during
in vitro carbonylation as well as for clinical practice.</description><subject>Benzoquinones - chemistry</subject><subject>Biological and medical sciences</subject><subject>Carbonylation</subject><subject>Case-Control Studies</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Female</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methylglyoxal</subject><subject>Protein amino group</subject><subject>Protein Carbonylation</subject><subject>Proteins - analysis</subject><subject>Proteins - chemistry</subject><subject>Proteins - metabolism</subject><subject>Pyruvaldehyde - chemistry</subject><subject>Pyruvaldehyde - metabolism</subject><subject>Serum Albumin - analysis</subject><subject>Serum Albumin - chemistry</subject><subject>Serum Albumin - metabolism</subject><subject>Spectrophotometric determination with p-benzoquinone</subject><subject>Spectrophotometry</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEuP0zAQgC0EYsvCX0DmgDgljBPbcY6o4iXtisveLXcyaV0ldrETpP33uLQ8jlxmNPI345mPsTcCagFCvz_WOPmw8xEPNNcNCFGDqkH0T9hGmK6tmr5tn7INAPRVLxq4YS9yPpaykUY_ZzeN0LK8wYbd39NyiAMfY-JzDH6JyYc9jyNfDsRPKS7kA3ezD5HvU1xPHA8u7CnzYf1Foku7GB4nt_gYXrJno5syvbrmW_bw6ePD9kt19-3z1-2Huwol6KUqAQw1ErvdSFp0KIxTkqgxrTTtqJSTQgkhFLaGtOx6HHunhxa1MrKcdsveXcaW_b6vlBc7-4w0TS5QXLM1nYEWFJzJ_kJiijknGu0p-dmlRyvAnl3ao_3HpT27tKBscVl6X19_WXczDX86f8srwNsr4DK6aUwuoM9_OSnLDbor3PbCUVHyw1OyGT0FpMEnwsUO0f_HOj8BxRKXsA</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>ACIMOVIC, Jelena M</creator><creator>JOVANOVIC, Vesna B</creator><creator>VESELINOVIC, Milica R</creator><creator>SRECKOVIC, Vesna Dimitrijevic</creator><creator>MANDIC, Ljuba M</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110801</creationdate><title>Method for monitoring of the protein amino group changes during carbonylation</title><author>ACIMOVIC, Jelena M ; JOVANOVIC, Vesna B ; VESELINOVIC, Milica R ; SRECKOVIC, Vesna Dimitrijevic ; MANDIC, Ljuba M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-40608e24c7bfe617c18a54ee283483f55a4151115c38e6479cf9a6d3c6584933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Benzoquinones - chemistry</topic><topic>Biological and medical sciences</topic><topic>Carbonylation</topic><topic>Case-Control Studies</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Type 2 - metabolism</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Female</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methylglyoxal</topic><topic>Protein amino group</topic><topic>Protein Carbonylation</topic><topic>Proteins - analysis</topic><topic>Proteins - chemistry</topic><topic>Proteins - metabolism</topic><topic>Pyruvaldehyde - chemistry</topic><topic>Pyruvaldehyde - metabolism</topic><topic>Serum Albumin - analysis</topic><topic>Serum Albumin - chemistry</topic><topic>Serum Albumin - metabolism</topic><topic>Spectrophotometric determination with p-benzoquinone</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ACIMOVIC, Jelena M</creatorcontrib><creatorcontrib>JOVANOVIC, Vesna B</creatorcontrib><creatorcontrib>VESELINOVIC, Milica R</creatorcontrib><creatorcontrib>SRECKOVIC, Vesna Dimitrijevic</creatorcontrib><creatorcontrib>MANDIC, Ljuba M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ACIMOVIC, Jelena M</au><au>JOVANOVIC, Vesna B</au><au>VESELINOVIC, Milica R</au><au>SRECKOVIC, Vesna Dimitrijevic</au><au>MANDIC, Ljuba M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Method for monitoring of the protein amino group changes during carbonylation</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>44</volume><issue>12</issue><spage>994</spage><epage>999</epage><pages>994-999</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation.
The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation
in vitro with methylglyoxal and
in vivo in 13 patients with type 2 diabetes and 20 healthy persons.
The method is simple, fast, precise (RSD in the range of 1.2–1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p
<
0.01) in comparison with a control group.
The method developed is suitable for quantification of protein amino groups during
in vitro carbonylation as well as for clinical practice.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>21640090</pmid><doi>10.1016/j.clinbiochem.2011.05.019</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9120 |
| ispartof | Clinical biochemistry, 2011-08, Vol.44 (12), p.994-999 |
| issn | 0009-9120 1873-2933 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_878030503 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Benzoquinones - chemistry Biological and medical sciences Carbonylation Case-Control Studies Diabetes Diabetes Mellitus, Type 2 - metabolism Diabetes. Impaired glucose tolerance Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Female Humans Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Methylglyoxal Protein amino group Protein Carbonylation Proteins - analysis Proteins - chemistry Proteins - metabolism Pyruvaldehyde - chemistry Pyruvaldehyde - metabolism Serum Albumin - analysis Serum Albumin - chemistry Serum Albumin - metabolism Spectrophotometric determination with p-benzoquinone Spectrophotometry |
| title | Method for monitoring of the protein amino group changes during carbonylation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T07%3A33%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Method%20for%20monitoring%20of%20the%20protein%20amino%20group%20changes%20during%20carbonylation&rft.jtitle=Clinical%20biochemistry&rft.au=ACIMOVIC,%20Jelena%20M&rft.date=2011-08-01&rft.volume=44&rft.issue=12&rft.spage=994&rft.epage=999&rft.pages=994-999&rft.issn=0009-9120&rft.eissn=1873-2933&rft_id=info:doi/10.1016/j.clinbiochem.2011.05.019&rft_dat=%3Cproquest_cross%3E878030503%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=878030503&rft_id=info:pmid/21640090&rft_els_id=S0009912011003705&rfr_iscdi=true |