Maintaining ATP levels via the suppression of PERK-mediated rRNA synthesis at ER stress

Currently, [3H]uridine is most often used to monitor rRNA synthesis in cultured cells. We show here that radiolabeled ribonucleoside triphosphates, such as [α-33P]UTP, in culture medium were also incorporated efficiently not only into cells but also into de novo RNA, particularly rRNA. Using this me...

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Veröffentlicht in:	Biochemical and biophysical research communications 2010-03, Vol.394 (1), p.42-47
Hauptverfasser:	Okamoto, Akihiko, Koike, Masaaki, Yasuda, Kunihiko, Kakizuka, Akira
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism eIF-2 Kinase - antagonists & inhibitors eIF-2 Kinase - metabolism eIF2α phosphorylation Endoplasmic Reticulum - enzymology ER stress HeLa Cells Humans PERK Protein Kinase Inhibitors - pharmacology Protein synthesis suppression RNA, Ribosomal - antagonists & inhibitors RNA, Ribosomal - biosynthesis rRNA synthesis suppression Stress, Physiological Thapsigargin - pharmacology Tunicamycin - pharmacology
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