Occurrence and genotyping using automated repetitive-sequence–based PCR of methicillin-resistant Staphylococcus aureus ST398 in Southeast Austria
Abstract In this retrospective study, the occurrence and genetic relatedness of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in Austrian MRSA patients was investigated. From 2002 to 2008, 14 MRSA ST398 were detected. First occurrence of MRSA ST398 was already found in 2004. Spa ribotypin...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2010-02, Vol.66 (2), p.217-221 |
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| creator | Grisold, Andrea J Zarfel, Gernot Hoenigl, Martin Krziwanek, Karina Feierl, Gebhard Masoud, Lilian Leitner, Eva Wagner-Eibel, Ute Badura, Alexandra Marth, Egon |
| description | Abstract In this retrospective study, the occurrence and genetic relatedness of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in Austrian MRSA patients was investigated. From 2002 to 2008, 14 MRSA ST398 were detected. First occurrence of MRSA ST398 was already found in 2004. Spa ribotyping assigned 12 isolates to spa type t011 and 1 each to spa type t034 and spa type t1451. Isolated MRSA ST398 was nontypeable by pulsed-field gel electrophoresis (NT-MRSA) using restriction enzyme Sma I; therefore, genotyping was performed using automated repetitive-sequence–based PCR (rep-PCR) on the DiversiLab system. Rep-PCR results assigned 10 (71%) of the 14 MRSA ST398 into 1 cluster with a similarity >95%; there was 1 cluster consisting of 2 isolates with a similarity >99% and 2 unique MRSA ST398 isolates. In conclusion, MRSA ST398 was continuously detected in Southeast Austria; first in 2004 with up to 5 MRSA ST398 isolates in 2008. Automated rep-PCR proved as a reliable technique in determining genetic relatedness of NT-MRSA ST398 and demonstrates clonal spread of MRSA ST398 in the investigated region. |
| doi_str_mv | 10.1016/j.diagmicrobio.2009.09.006 |
| format | Article |
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From 2002 to 2008, 14 MRSA ST398 were detected. First occurrence of MRSA ST398 was already found in 2004. Spa ribotyping assigned 12 isolates to spa type t011 and 1 each to spa type t034 and spa type t1451. Isolated MRSA ST398 was nontypeable by pulsed-field gel electrophoresis (NT-MRSA) using restriction enzyme Sma I; therefore, genotyping was performed using automated repetitive-sequence–based PCR (rep-PCR) on the DiversiLab system. Rep-PCR results assigned 10 (71%) of the 14 MRSA ST398 into 1 cluster with a similarity >95%; there was 1 cluster consisting of 2 isolates with a similarity >99% and 2 unique MRSA ST398 isolates. In conclusion, MRSA ST398 was continuously detected in Southeast Austria; first in 2004 with up to 5 MRSA ST398 isolates in 2008. Automated rep-PCR proved as a reliable technique in determining genetic relatedness of NT-MRSA ST398 and demonstrates clonal spread of MRSA ST398 in the investigated region.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2009.09.006</identifier><identifier>PMID: 19828273</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Aged ; Animals ; Austria ; Automation ; Bacterial Typing Techniques - methods ; Bacteriology ; Biological and medical sciences ; Child ; Child, Preschool ; Cluster Analysis ; DiversiLab Microbial Typing System ; DNA Fingerprinting - methods ; DNA, Bacterial - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Genotype ; Genotyping ; Humans ; Infant ; Infectious Disease ; Infectious diseases ; Internal Medicine ; Male ; Medical sciences ; Methicillin-resistant Staphylococcus aureus ; Methicillin-Resistant Staphylococcus aureus - classification ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Microbiology ; Middle Aged ; Miscellaneous ; Molecular Epidemiology ; MRSA ST398 ; Pig ; Polymerase Chain Reaction - methods ; Repetitive Sequences, Nucleic Acid ; Repetitive-sequence-based PCR ; Retrospective Studies ; Spa typing ; Staphylococcal Infections - microbiology ; Staphylococcus aureus</subject><ispartof>Diagnostic microbiology and infectious disease, 2010-02, Vol.66 (2), p.217-221</ispartof><rights>Elsevier Inc.</rights><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-1b0806fc85d7bb730c18d6cf247737bbc8b8571681aa928d0c93e25ed42bda6e3</citedby><cites>FETCH-LOGICAL-c496t-1b0806fc85d7bb730c18d6cf247737bbc8b8571681aa928d0c93e25ed42bda6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.diagmicrobio.2009.09.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22366875$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19828273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grisold, Andrea J</creatorcontrib><creatorcontrib>Zarfel, Gernot</creatorcontrib><creatorcontrib>Hoenigl, Martin</creatorcontrib><creatorcontrib>Krziwanek, Karina</creatorcontrib><creatorcontrib>Feierl, Gebhard</creatorcontrib><creatorcontrib>Masoud, Lilian</creatorcontrib><creatorcontrib>Leitner, Eva</creatorcontrib><creatorcontrib>Wagner-Eibel, Ute</creatorcontrib><creatorcontrib>Badura, Alexandra</creatorcontrib><creatorcontrib>Marth, Egon</creatorcontrib><title>Occurrence and genotyping using automated repetitive-sequence–based PCR of methicillin-resistant Staphylococcus aureus ST398 in Southeast Austria</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract In this retrospective study, the occurrence and genetic relatedness of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in Austrian MRSA patients was investigated. From 2002 to 2008, 14 MRSA ST398 were detected. First occurrence of MRSA ST398 was already found in 2004. Spa ribotyping assigned 12 isolates to spa type t011 and 1 each to spa type t034 and spa type t1451. Isolated MRSA ST398 was nontypeable by pulsed-field gel electrophoresis (NT-MRSA) using restriction enzyme Sma I; therefore, genotyping was performed using automated repetitive-sequence–based PCR (rep-PCR) on the DiversiLab system. Rep-PCR results assigned 10 (71%) of the 14 MRSA ST398 into 1 cluster with a similarity >95%; there was 1 cluster consisting of 2 isolates with a similarity >99% and 2 unique MRSA ST398 isolates. In conclusion, MRSA ST398 was continuously detected in Southeast Austria; first in 2004 with up to 5 MRSA ST398 isolates in 2008. Automated rep-PCR proved as a reliable technique in determining genetic relatedness of NT-MRSA ST398 and demonstrates clonal spread of MRSA ST398 in the investigated region.</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Austria</subject><subject>Automation</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cluster Analysis</subject><subject>DiversiLab Microbial Typing System</subject><subject>DNA Fingerprinting - methods</subject><subject>DNA, Bacterial - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Genotyping</subject><subject>Humans</subject><subject>Infant</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methicillin-resistant Staphylococcus aureus</subject><subject>Methicillin-Resistant Staphylococcus aureus - classification</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>MRSA ST398</subject><subject>Pig</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Repetitive-sequence-based PCR</subject><subject>Retrospective Studies</subject><subject>Spa typing</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt-KEzEUxgdR3O7qK8ggiFdTT5J2knghLHX9Awsrdr0OmeRMmzozqUlmoXe-w76hT2KGFhVvFA45kPy-c5J8pyieE5gTIPWr3dw6vemdCb5xfk4B5HwKqB8UMyK4rAA4PCxmwBmthJDsrDiPcQdAqFzA4-KMSEEF5WxW3N8YM4aAg8FSD7bc4ODTYe-GTTnGadVj8r1OaMuAe0wuuTusIn4bJ8mP7_eNjvns0-pz6duyx7R1xnWdG6qA0cWkh1Suk95vD503PveKuWLAnNa3TIrSDeXaj2mLOqbycowpOP2keNTqLuLTU74ovry7ul19qK5v3n9cXV5XZiHrVJEGBNStEUvLm4YzMETY2rR0wTnLO0Y0YslJLYjWkgoLRjKkS7QL2lhdI7soXh7r7oPP74lJ9S4a7Do9oB-jEpxnPVD2T5IzJpaCiIl8fSSzOTEGbNU-uF6HgyKgJvfUTv3pnprcU1NAncXPTm3Gpkf7W3qyKwMvToCORndt0INx8RdHKatrwZeZe3vkMH_fncOgonGTYdYFNElZ7_7vPm_-KmOysy53_ooHjDs_hiEbpIiKVIFaT_M2jRtIAFZLwn4CO_XYWg</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Grisold, Andrea J</creator><creator>Zarfel, Gernot</creator><creator>Hoenigl, Martin</creator><creator>Krziwanek, Karina</creator><creator>Feierl, Gebhard</creator><creator>Masoud, Lilian</creator><creator>Leitner, Eva</creator><creator>Wagner-Eibel, Ute</creator><creator>Badura, Alexandra</creator><creator>Marth, Egon</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20100201</creationdate><title>Occurrence and genotyping using automated repetitive-sequence–based PCR of methicillin-resistant Staphylococcus aureus ST398 in Southeast Austria</title><author>Grisold, Andrea J ; Zarfel, Gernot ; Hoenigl, Martin ; Krziwanek, Karina ; Feierl, Gebhard ; Masoud, Lilian ; Leitner, Eva ; Wagner-Eibel, Ute ; Badura, Alexandra ; Marth, Egon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-1b0806fc85d7bb730c18d6cf247737bbc8b8571681aa928d0c93e25ed42bda6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Austria</topic><topic>Automation</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cluster Analysis</topic><topic>DiversiLab Microbial Typing System</topic><topic>DNA Fingerprinting - methods</topic><topic>DNA, Bacterial - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Genotyping</topic><topic>Humans</topic><topic>Infant</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methicillin-resistant Staphylococcus aureus</topic><topic>Methicillin-Resistant Staphylococcus aureus - classification</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation & purification</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>MRSA ST398</topic><topic>Pig</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Repetitive-sequence-based PCR</topic><topic>Retrospective Studies</topic><topic>Spa typing</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grisold, Andrea J</creatorcontrib><creatorcontrib>Zarfel, Gernot</creatorcontrib><creatorcontrib>Hoenigl, Martin</creatorcontrib><creatorcontrib>Krziwanek, Karina</creatorcontrib><creatorcontrib>Feierl, Gebhard</creatorcontrib><creatorcontrib>Masoud, Lilian</creatorcontrib><creatorcontrib>Leitner, Eva</creatorcontrib><creatorcontrib>Wagner-Eibel, Ute</creatorcontrib><creatorcontrib>Badura, Alexandra</creatorcontrib><creatorcontrib>Marth, Egon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grisold, Andrea J</au><au>Zarfel, Gernot</au><au>Hoenigl, Martin</au><au>Krziwanek, Karina</au><au>Feierl, Gebhard</au><au>Masoud, Lilian</au><au>Leitner, Eva</au><au>Wagner-Eibel, Ute</au><au>Badura, Alexandra</au><au>Marth, Egon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and genotyping using automated repetitive-sequence–based PCR of methicillin-resistant Staphylococcus aureus ST398 in Southeast Austria</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>66</volume><issue>2</issue><spage>217</spage><epage>221</epage><pages>217-221</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Abstract In this retrospective study, the occurrence and genetic relatedness of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in Austrian MRSA patients was investigated. From 2002 to 2008, 14 MRSA ST398 were detected. First occurrence of MRSA ST398 was already found in 2004. Spa ribotyping assigned 12 isolates to spa type t011 and 1 each to spa type t034 and spa type t1451. Isolated MRSA ST398 was nontypeable by pulsed-field gel electrophoresis (NT-MRSA) using restriction enzyme Sma I; therefore, genotyping was performed using automated repetitive-sequence–based PCR (rep-PCR) on the DiversiLab system. Rep-PCR results assigned 10 (71%) of the 14 MRSA ST398 into 1 cluster with a similarity >95%; there was 1 cluster consisting of 2 isolates with a similarity >99% and 2 unique MRSA ST398 isolates. In conclusion, MRSA ST398 was continuously detected in Southeast Austria; first in 2004 with up to 5 MRSA ST398 isolates in 2008. Automated rep-PCR proved as a reliable technique in determining genetic relatedness of NT-MRSA ST398 and demonstrates clonal spread of MRSA ST398 in the investigated region.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19828273</pmid><doi>10.1016/j.diagmicrobio.2009.09.006</doi><tpages>5</tpages></addata></record> |
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| subjects | Adult Aged Animals Austria Automation Bacterial Typing Techniques - methods Bacteriology Biological and medical sciences Child Child, Preschool Cluster Analysis DiversiLab Microbial Typing System DNA Fingerprinting - methods DNA, Bacterial - genetics Female Fundamental and applied biological sciences. Psychology Genotype Genotyping Humans Infant Infectious Disease Infectious diseases Internal Medicine Male Medical sciences Methicillin-resistant Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus - classification Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - isolation & purification Microbiology Middle Aged Miscellaneous Molecular Epidemiology MRSA ST398 Pig Polymerase Chain Reaction - methods Repetitive Sequences, Nucleic Acid Repetitive-sequence-based PCR Retrospective Studies Spa typing Staphylococcal Infections - microbiology Staphylococcus aureus |
| title | Occurrence and genotyping using automated repetitive-sequence–based PCR of methicillin-resistant Staphylococcus aureus ST398 in Southeast Austria |
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