Nanostructured poly(3,4-ethylenedioxythiophene)–metalloporphyrin films: Improved catalytic detection of peroxynitrite
We investigated in this paper the sensing performance of inherently conductive polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), functionalized with hemin (iron protoporphyrin) as an electrocatalytic reporter. The sensing platform is prepared by electrodeposition of a composite film of hemin–PEDOT...
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| Veröffentlicht in: | Biosensors & bioelectronics 2010-04, Vol.25 (8), p.1914-1921 |
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| container_title | Biosensors & bioelectronics |
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| creator | Peteu, Serban Peiris, Pubudu Gebremichael, Ermias Bayachou, Mekki |
| description | We investigated in this paper the sensing performance of inherently conductive polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), functionalized with hemin (iron protoporphyrin) as an electrocatalytic reporter. The sensing platform is prepared by electrodeposition of a composite film of hemin–PEDOT on a 30-μm diameter carbon fiber electrode (CFE). The polymerized films were characterized by field emission scanning electron microscopy (FESEM), which pointed to nanostructured films with tortuous pores. The electrocatalytic oxidation of peroxynitrite was characterized by cyclic voltammetry as well as other electrochemical methods. The catalytic current is proportional to the analyte's concentration. Optimized hemin–PEDOT modified CFEs were utilized for the first time to detect ONO
2
-, with a response time down to 5
s and a limit of detection as low as 200
nM as evidenced by amperometry. Our hemin–PEDOT modified CFEs have a sensitivity of 13
nA/μM,
ca.130 times higher than the bare CFE. More work is underway using other metalloporphyrins as electrocalalysts to improve the detection limit, the selectivity, and to further miniaturize these hemin–PEDOT modified electrodes. |
| doi_str_mv | 10.1016/j.bios.2010.01.008 |
| format | Article |
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2
-, with a response time down to 5
s and a limit of detection as low as 200
nM as evidenced by amperometry. Our hemin–PEDOT modified CFEs have a sensitivity of 13
nA/μM,
ca.130 times higher than the bare CFE. More work is underway using other metalloporphyrins as electrocalalysts to improve the detection limit, the selectivity, and to further miniaturize these hemin–PEDOT modified electrodes.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2010.01.008</identifier><identifier>PMID: 20129772</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; Bridged Bicyclo Compounds, Heterocyclic - chemistry ; Carbon fiber microelectrode ; Catalysis ; Conductometry - instrumentation ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Membranes, Artificial ; Metalloporphyrin ; Nanostructure ; Nanostructures - chemistry ; Nanostructures - ultrastructure ; Nanotechnology - instrumentation ; Peroxynitrite detection ; Peroxynitrous Acid - analysis ; Peroxynitrous Acid - chemistry ; Poly(3,4-ethylenedioxythiophene) (PEDOT) ; Polymers - chemistry ; Protoporphyrins - chemistry ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Biosensors & bioelectronics, 2010-04, Vol.25 (8), p.1914-1921</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e9b8227b0f7c9f95ca6793f94fdb88b871013c1e59137985cc0240fa2d8b1e83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2010.01.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22566235$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20129772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peteu, Serban</creatorcontrib><creatorcontrib>Peiris, Pubudu</creatorcontrib><creatorcontrib>Gebremichael, Ermias</creatorcontrib><creatorcontrib>Bayachou, Mekki</creatorcontrib><title>Nanostructured poly(3,4-ethylenedioxythiophene)–metalloporphyrin films: Improved catalytic detection of peroxynitrite</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>We investigated in this paper the sensing performance of inherently conductive polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), functionalized with hemin (iron protoporphyrin) as an electrocatalytic reporter. The sensing platform is prepared by electrodeposition of a composite film of hemin–PEDOT on a 30-μm diameter carbon fiber electrode (CFE). The polymerized films were characterized by field emission scanning electron microscopy (FESEM), which pointed to nanostructured films with tortuous pores. The electrocatalytic oxidation of peroxynitrite was characterized by cyclic voltammetry as well as other electrochemical methods. The catalytic current is proportional to the analyte's concentration. Optimized hemin–PEDOT modified CFEs were utilized for the first time to detect ONO
2
-, with a response time down to 5
s and a limit of detection as low as 200
nM as evidenced by amperometry. Our hemin–PEDOT modified CFEs have a sensitivity of 13
nA/μM,
ca.130 times higher than the bare CFE. More work is underway using other metalloporphyrins as electrocalalysts to improve the detection limit, the selectivity, and to further miniaturize these hemin–PEDOT modified electrodes.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>Bridged Bicyclo Compounds, Heterocyclic - chemistry</subject><subject>Carbon fiber microelectrode</subject><subject>Catalysis</subject><subject>Conductometry - instrumentation</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Membranes, Artificial</subject><subject>Metalloporphyrin</subject><subject>Nanostructure</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - ultrastructure</subject><subject>Nanotechnology - instrumentation</subject><subject>Peroxynitrite detection</subject><subject>Peroxynitrous Acid - analysis</subject><subject>Peroxynitrous Acid - chemistry</subject><subject>Poly(3,4-ethylenedioxythiophene) (PEDOT)</subject><subject>Polymers - chemistry</subject><subject>Protoporphyrins - chemistry</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EokPhBVigbBAgkcGXJLZRN1XFpVIFm-4txznWeOTEwXYK2fEOvCFPgkczwA5W1rG-8-vo_xB6SvCWYNK92W97F9KW4vKByRZjcQ9tiOCsbihr76MNlm1Xt13HztCjlPYYY04kfojOygqVnNMN-vpJTyHluJi8RBiqOfj1JXvd1JB3q4cJBhe-rXnnwrwr06uf33-MkLX3YQ5x3q3RTZV1fkxvq-txjuGuZBhdgDU7Uw2QwWQXpirYaoZYoiaXo8vwGD2w2id4cnrP0e37d7dXH-ubzx-ury5vatMQnmuQvaCU99hyI61sje64ZFY2duiF6AUvRTBDoJWEcSlaYzBtsNV0ED0Bwc7Ri2NsOe3LAimr0SUD3usJwpKU4LztpGj4f0nOGGWkkQeSHkkTQ0oRrJqjG3VcFcHqIEbt1UGMOohRmKgipiw9O8Uv_QjDn5XfJgrw_AToZLS3UU_Gpb8cLR6L1cJdHDkord05iCoZB5MpomLpWg3B_euOX36Tr7k</recordid><startdate>20100415</startdate><enddate>20100415</enddate><creator>Peteu, Serban</creator><creator>Peiris, Pubudu</creator><creator>Gebremichael, Ermias</creator><creator>Bayachou, Mekki</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100415</creationdate><title>Nanostructured poly(3,4-ethylenedioxythiophene)–metalloporphyrin films: Improved catalytic detection of peroxynitrite</title><author>Peteu, Serban ; Peiris, Pubudu ; Gebremichael, Ermias ; Bayachou, Mekki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e9b8227b0f7c9f95ca6793f94fdb88b871013c1e59137985cc0240fa2d8b1e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>Bridged Bicyclo Compounds, Heterocyclic - chemistry</topic><topic>Carbon fiber microelectrode</topic><topic>Catalysis</topic><topic>Conductometry - instrumentation</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Membranes, Artificial</topic><topic>Metalloporphyrin</topic><topic>Nanostructure</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - ultrastructure</topic><topic>Nanotechnology - instrumentation</topic><topic>Peroxynitrite detection</topic><topic>Peroxynitrous Acid - analysis</topic><topic>Peroxynitrous Acid - chemistry</topic><topic>Poly(3,4-ethylenedioxythiophene) (PEDOT)</topic><topic>Polymers - chemistry</topic><topic>Protoporphyrins - chemistry</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peteu, Serban</creatorcontrib><creatorcontrib>Peiris, Pubudu</creatorcontrib><creatorcontrib>Gebremichael, Ermias</creatorcontrib><creatorcontrib>Bayachou, Mekki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peteu, Serban</au><au>Peiris, Pubudu</au><au>Gebremichael, Ermias</au><au>Bayachou, Mekki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanostructured poly(3,4-ethylenedioxythiophene)–metalloporphyrin films: Improved catalytic detection of peroxynitrite</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2010-04-15</date><risdate>2010</risdate><volume>25</volume><issue>8</issue><spage>1914</spage><epage>1921</epage><pages>1914-1921</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>We investigated in this paper the sensing performance of inherently conductive polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), functionalized with hemin (iron protoporphyrin) as an electrocatalytic reporter. The sensing platform is prepared by electrodeposition of a composite film of hemin–PEDOT on a 30-μm diameter carbon fiber electrode (CFE). The polymerized films were characterized by field emission scanning electron microscopy (FESEM), which pointed to nanostructured films with tortuous pores. The electrocatalytic oxidation of peroxynitrite was characterized by cyclic voltammetry as well as other electrochemical methods. The catalytic current is proportional to the analyte's concentration. Optimized hemin–PEDOT modified CFEs were utilized for the first time to detect ONO
2
-, with a response time down to 5
s and a limit of detection as low as 200
nM as evidenced by amperometry. Our hemin–PEDOT modified CFEs have a sensitivity of 13
nA/μM,
ca.130 times higher than the bare CFE. More work is underway using other metalloporphyrins as electrocalalysts to improve the detection limit, the selectivity, and to further miniaturize these hemin–PEDOT modified electrodes.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>20129772</pmid><doi>10.1016/j.bios.2010.01.008</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2010-04, Vol.25 (8), p.1914-1921 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology Bridged Bicyclo Compounds, Heterocyclic - chemistry Carbon fiber microelectrode Catalysis Conductometry - instrumentation Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Membranes, Artificial Metalloporphyrin Nanostructure Nanostructures - chemistry Nanostructures - ultrastructure Nanotechnology - instrumentation Peroxynitrite detection Peroxynitrous Acid - analysis Peroxynitrous Acid - chemistry Poly(3,4-ethylenedioxythiophene) (PEDOT) Polymers - chemistry Protoporphyrins - chemistry Reproducibility of Results Sensitivity and Specificity |
| title | Nanostructured poly(3,4-ethylenedioxythiophene)–metalloporphyrin films: Improved catalytic detection of peroxynitrite |
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