Encapsulation of proteins in poly( l-lactide-co-caprolactone) fibers by emulsion electrospinning
This study was aimed at investigating emulsion electrospinning to prepare biodegradable fibrous mats with encapsulation of human-nerve growth factor (NGF). One of the best methods for fabricating a bio-functional tissue engineering scaffold is to load bioactive agent into the scaffold. In this work,...
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| Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2010-02, Vol.75 (2), p.418-424 |
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| container_title | Colloids and surfaces, B, Biointerfaces |
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| creator | Li, Xiaoqiang Su, Yan Liu, Shuiping Tan, Lianjiang Mo, Xiumei Ramakrishna, Seeram |
| description | This study was aimed at investigating emulsion electrospinning to prepare biodegradable fibrous mats with encapsulation of human-nerve growth factor (NGF). One of the best methods for fabricating a bio-functional tissue engineering scaffold is to load bioactive agent into the scaffold. In this work, the feasibility of incorporating NGF into poly(
l-lactide-co-caprolactone) fibers by emulsion electrospinning has been studied. The release behavior of encapsulated bovine serum albumin (BSA) was investigated. The bioactivity of NGF released from fibrous mats was verified by testing the neurite outgrowth of rat pheochromocytoma cells (PC12). Furthermore, the process of fiber forming during emulsion electrospinning was discussed. The results demonstrate that emulsion electrospun fibers can successfully encapsulate proteins and release them in a sustained manner. The bioactivity of NGF released from emulsion electrospun fibers was confirmed by PC12 bioassays. |
| doi_str_mv | 10.1016/j.colsurfb.2009.09.014 |
| format | Article |
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l-lactide-co-caprolactone) fibers by emulsion electrospinning has been studied. The release behavior of encapsulated bovine serum albumin (BSA) was investigated. The bioactivity of NGF released from fibrous mats was verified by testing the neurite outgrowth of rat pheochromocytoma cells (PC12). Furthermore, the process of fiber forming during emulsion electrospinning was discussed. The results demonstrate that emulsion electrospun fibers can successfully encapsulate proteins and release them in a sustained manner. The bioactivity of NGF released from emulsion electrospun fibers was confirmed by PC12 bioassays.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2009.09.014</identifier><identifier>PMID: 19836931</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cattle ; Controlled release ; Emulsion electrospinning ; Emulsions ; Humans ; Microscopy, Electron, Scanning ; Models, Chemical ; Nerve Growth Factor - pharmacology ; PC12 Cells ; PLACL ; Polyesters - chemistry ; Rats ; Serum Albumin, Bovine - chemistry ; Solubility - drug effects ; Tissue engineering ; Tissue Engineering - methods ; Water - chemistry</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2010-02, Vol.75 (2), p.418-424</ispartof><rights>2009 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-9c72b172a83e6b1cc6b8a3e962ef5507241b6533d2316febba5cb82e77b917e43</citedby><cites>FETCH-LOGICAL-c465t-9c72b172a83e6b1cc6b8a3e962ef5507241b6533d2316febba5cb82e77b917e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0927776509004469$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19836931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiaoqiang</creatorcontrib><creatorcontrib>Su, Yan</creatorcontrib><creatorcontrib>Liu, Shuiping</creatorcontrib><creatorcontrib>Tan, Lianjiang</creatorcontrib><creatorcontrib>Mo, Xiumei</creatorcontrib><creatorcontrib>Ramakrishna, Seeram</creatorcontrib><title>Encapsulation of proteins in poly( l-lactide-co-caprolactone) fibers by emulsion electrospinning</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>This study was aimed at investigating emulsion electrospinning to prepare biodegradable fibrous mats with encapsulation of human-nerve growth factor (NGF). One of the best methods for fabricating a bio-functional tissue engineering scaffold is to load bioactive agent into the scaffold. In this work, the feasibility of incorporating NGF into poly(
l-lactide-co-caprolactone) fibers by emulsion electrospinning has been studied. The release behavior of encapsulated bovine serum albumin (BSA) was investigated. The bioactivity of NGF released from fibrous mats was verified by testing the neurite outgrowth of rat pheochromocytoma cells (PC12). Furthermore, the process of fiber forming during emulsion electrospinning was discussed. The results demonstrate that emulsion electrospun fibers can successfully encapsulate proteins and release them in a sustained manner. The bioactivity of NGF released from emulsion electrospun fibers was confirmed by PC12 bioassays.</description><subject>Animals</subject><subject>Cattle</subject><subject>Controlled release</subject><subject>Emulsion electrospinning</subject><subject>Emulsions</subject><subject>Humans</subject><subject>Microscopy, Electron, Scanning</subject><subject>Models, Chemical</subject><subject>Nerve Growth Factor - pharmacology</subject><subject>PC12 Cells</subject><subject>PLACL</subject><subject>Polyesters - chemistry</subject><subject>Rats</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Solubility - drug effects</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Water - chemistry</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtLxDAQhYMoul7-gvRNfeiaS5s0b4p4A8EXfY5JOpUs2aQmrbD_3pZd8VEYGAa-zJycg9A5wUuCCb9eLW30eUydWVKM5XIuUu2hBWkEKyvGxT5aYElFKQSvj9BxziuMMa2IOERHRDaMS0YW6OM-WN3n0evBxVDEruhTHMCFXLhQ9NFvLgtfem0H10JpYznRKc5zDHBVdM5AyoXZFLAefZ5XgAc7pJh7F4ILn6fooNM-w9mun6D3h_u3u6fy5fXx-e72pbQVr4dSWkENEVQ3DLgh1nLTaAaSU-jqGotJuOE1Yy1lhHdgjK6taSgIYSQRULETdLHdO8n7GiEPau2yBe91gDhm1QhRc8kF_5cUrCK1JJhOJN-SdvpPTtCpPrm1ThtFsJpjUCv1G4OaY1BzkVnM-e7EaNbQ_j3b-T4BN1sAJku-HSSVrYNgoXVpsk-10f134wd6hZ3Y</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Li, Xiaoqiang</creator><creator>Su, Yan</creator><creator>Liu, Shuiping</creator><creator>Tan, Lianjiang</creator><creator>Mo, Xiumei</creator><creator>Ramakrishna, Seeram</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100201</creationdate><title>Encapsulation of proteins in poly( l-lactide-co-caprolactone) fibers by emulsion electrospinning</title><author>Li, Xiaoqiang ; Su, Yan ; Liu, Shuiping ; Tan, Lianjiang ; Mo, Xiumei ; Ramakrishna, Seeram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-9c72b172a83e6b1cc6b8a3e962ef5507241b6533d2316febba5cb82e77b917e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Controlled release</topic><topic>Emulsion electrospinning</topic><topic>Emulsions</topic><topic>Humans</topic><topic>Microscopy, Electron, Scanning</topic><topic>Models, Chemical</topic><topic>Nerve Growth Factor - pharmacology</topic><topic>PC12 Cells</topic><topic>PLACL</topic><topic>Polyesters - chemistry</topic><topic>Rats</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Solubility - drug effects</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiaoqiang</creatorcontrib><creatorcontrib>Su, Yan</creatorcontrib><creatorcontrib>Liu, Shuiping</creatorcontrib><creatorcontrib>Tan, Lianjiang</creatorcontrib><creatorcontrib>Mo, Xiumei</creatorcontrib><creatorcontrib>Ramakrishna, Seeram</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiaoqiang</au><au>Su, Yan</au><au>Liu, Shuiping</au><au>Tan, Lianjiang</au><au>Mo, Xiumei</au><au>Ramakrishna, Seeram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Encapsulation of proteins in poly( l-lactide-co-caprolactone) fibers by emulsion electrospinning</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>75</volume><issue>2</issue><spage>418</spage><epage>424</epage><pages>418-424</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>This study was aimed at investigating emulsion electrospinning to prepare biodegradable fibrous mats with encapsulation of human-nerve growth factor (NGF). One of the best methods for fabricating a bio-functional tissue engineering scaffold is to load bioactive agent into the scaffold. In this work, the feasibility of incorporating NGF into poly(
l-lactide-co-caprolactone) fibers by emulsion electrospinning has been studied. The release behavior of encapsulated bovine serum albumin (BSA) was investigated. The bioactivity of NGF released from fibrous mats was verified by testing the neurite outgrowth of rat pheochromocytoma cells (PC12). Furthermore, the process of fiber forming during emulsion electrospinning was discussed. The results demonstrate that emulsion electrospun fibers can successfully encapsulate proteins and release them in a sustained manner. The bioactivity of NGF released from emulsion electrospun fibers was confirmed by PC12 bioassays.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19836931</pmid><doi>10.1016/j.colsurfb.2009.09.014</doi><tpages>7</tpages></addata></record> |
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| ispartof | Colloids and surfaces, B, Biointerfaces, 2010-02, Vol.75 (2), p.418-424 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cattle Controlled release Emulsion electrospinning Emulsions Humans Microscopy, Electron, Scanning Models, Chemical Nerve Growth Factor - pharmacology PC12 Cells PLACL Polyesters - chemistry Rats Serum Albumin, Bovine - chemistry Solubility - drug effects Tissue engineering Tissue Engineering - methods Water - chemistry |
| title | Encapsulation of proteins in poly( l-lactide-co-caprolactone) fibers by emulsion electrospinning |
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