One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer
Purpose This study was designed to apply safely the sentinel node navigation surgery (SNNS) to the malignancies, an accurate and prompt intraoperative diagnosis of SN is essential, and micrometastasis has been frequently missed by conventional frozen sections. Recently, a novel molecular-based rapid...
Gespeichert in:
| Veröffentlicht in: | Annals of surgical oncology 2011-08, Vol.18 (8), p.2289-2296 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2296 |
|---|---|
| container_issue | 8 |
| container_start_page | 2289 |
| container_title | Annals of surgical oncology |
| container_volume | 18 |
| creator | Yaguchi, Yoshihisa Sugasawa, Hidekazu Tsujimoto, Hironori Takata, Hideki Nakabayashi, Kadzuki Ichikura, Takashi Ono, Satoshi Hiraki, Shuichi Sakamoto, Naoko Horio, Takuya Kumano, Isao Otomo, Yasuhiro Mochizuki, Hidetaka Yamamoto, Junji Hase, Kazuo |
| description | Purpose
This study was designed to apply safely the sentinel node navigation surgery (SNNS) to the malignancies, an accurate and prompt intraoperative diagnosis of SN is essential, and micrometastasis has been frequently missed by conventional frozen sections. Recently, a novel molecular-based rapid diagnosis for the lymph node (LN) metastases has been developed using (OSNA) in breast cancer, which takes approximately 30 min to obtain a final result. We evaluated the efficacy of OSNA in terms of the intraoperative diagnosis of LN metastasis in patients with gastric cancer.
Methods
A total of 162 LNs dissected from 32 patients with gastric cancer was included in this study; 45 LNs were pathologically diagnosed as metastatic LNs and 117 LNs were negative. The LNs were bisected; halves were examined with H&E stain, and the opposite halves were subjected to OSNA analyses of CK19 mRNA. The CK19 mRNA expression was examined in the positive or negative metastatic LNs, and the correlation between the tumor volume and CK19 mRNA expression in the metastatic LNs was examined.
Results
The CK19 mRNA expressions in the positive metastatic LNs were significantly higher than those of negative LNs. When 250 copies/μl was set as a cutoff value, the concordance rate was 94.4%, the sensitivity was 88.9%, and the specificity was 96.6%. The OSNA expression was significantly correlated with the estimated tumor volumes in the metastatic LNs.
Conclusions
The OSNA method is feasible and acceptable for detecting LN metastases in patients with gastric cancer. This should be applied for the intraoperative diagnosis in the SN-navigation surgery in gastric cancer. |
| doi_str_mv | 10.1245/s10434-011-1591-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_877413494</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2399034011</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-b8d82c249a2cfd1f3ceb1461484b1233ccc396752d4211b91b9230ee2ae1abca3</originalsourceid><addsrcrecordid>eNp9kUFrFDEUx4MotlY_gBcJXqyHqXlJJpMch0WrUHYPW89DJvNGU2aTMZk5-O2bsquC0EIg4b3f-z3Cn5C3wK6Ay_pTBiaFrBhABbWByjwj51CXilQanpc3U7oyXNVn5FXOd4xBI1j9kpxxEAyM0ufE7wJW-wVnul3dhN7R1vmBtod58qN3dvEx0Mvdftt-pGNMdPmJtJ1L89SKI91jWHzAiW7jgHQTg8N5oT7Qa5uXVIwbW0rpNXkx2injm9N9Qb5_-Xy7-Vrd7K6_bdqbyklllqrXg-aOS2O5GwcYhcMepAKpZQ9cCOecMKqp-SA5QG_K4YIhcotge2fFBflw9M4p_loxL93BZ4fTZAPGNXe6aSQIaWQhL58kgTGteS0UL-j7_9C7uKZQ_lF8qmGN0VAgOEIuxZwTjt2c_MGm38XUPQTWHQPrSmDdQ2CdKTPvTuK1P-Dwd-JPQgXgRyCXVviB6d_mx633EvWeRQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>876707981</pqid></control><display><type>article</type><title>One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Yaguchi, Yoshihisa ; Sugasawa, Hidekazu ; Tsujimoto, Hironori ; Takata, Hideki ; Nakabayashi, Kadzuki ; Ichikura, Takashi ; Ono, Satoshi ; Hiraki, Shuichi ; Sakamoto, Naoko ; Horio, Takuya ; Kumano, Isao ; Otomo, Yasuhiro ; Mochizuki, Hidetaka ; Yamamoto, Junji ; Hase, Kazuo</creator><creatorcontrib>Yaguchi, Yoshihisa ; Sugasawa, Hidekazu ; Tsujimoto, Hironori ; Takata, Hideki ; Nakabayashi, Kadzuki ; Ichikura, Takashi ; Ono, Satoshi ; Hiraki, Shuichi ; Sakamoto, Naoko ; Horio, Takuya ; Kumano, Isao ; Otomo, Yasuhiro ; Mochizuki, Hidetaka ; Yamamoto, Junji ; Hase, Kazuo</creatorcontrib><description>Purpose
This study was designed to apply safely the sentinel node navigation surgery (SNNS) to the malignancies, an accurate and prompt intraoperative diagnosis of SN is essential, and micrometastasis has been frequently missed by conventional frozen sections. Recently, a novel molecular-based rapid diagnosis for the lymph node (LN) metastases has been developed using (OSNA) in breast cancer, which takes approximately 30 min to obtain a final result. We evaluated the efficacy of OSNA in terms of the intraoperative diagnosis of LN metastasis in patients with gastric cancer.
Methods
A total of 162 LNs dissected from 32 patients with gastric cancer was included in this study; 45 LNs were pathologically diagnosed as metastatic LNs and 117 LNs were negative. The LNs were bisected; halves were examined with H&E stain, and the opposite halves were subjected to OSNA analyses of CK19 mRNA. The CK19 mRNA expression was examined in the positive or negative metastatic LNs, and the correlation between the tumor volume and CK19 mRNA expression in the metastatic LNs was examined.
Results
The CK19 mRNA expressions in the positive metastatic LNs were significantly higher than those of negative LNs. When 250 copies/μl was set as a cutoff value, the concordance rate was 94.4%, the sensitivity was 88.9%, and the specificity was 96.6%. The OSNA expression was significantly correlated with the estimated tumor volumes in the metastatic LNs.
Conclusions
The OSNA method is feasible and acceptable for detecting LN metastases in patients with gastric cancer. This should be applied for the intraoperative diagnosis in the SN-navigation surgery in gastric cancer.</description><identifier>ISSN: 1068-9265</identifier><identifier>EISSN: 1534-4681</identifier><identifier>DOI: 10.1245/s10434-011-1591-9</identifier><identifier>PMID: 21301968</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Blotting, Western ; Feasibility Studies ; Female ; Follow-Up Studies ; Gastrectomy ; Gastrointestinal Oncology ; Humans ; Intestinal Neoplasms - diagnosis ; Intestinal Neoplasms - surgery ; Keratin-19 - genetics ; Keratin-19 - metabolism ; Lymph Nodes - pathology ; Lymph Nodes - surgery ; Lymphatic Metastasis ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Monitoring, Intraoperative ; Nucleic Acid Amplification Techniques ; Oncology ; Prognosis ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Sentinel Lymph Node Biopsy ; Stomach Neoplasms - diagnosis ; Stomach Neoplasms - surgery ; Surgery ; Surgical Oncology ; Survival Rate</subject><ispartof>Annals of surgical oncology, 2011-08, Vol.18 (8), p.2289-2296</ispartof><rights>Society of Surgical Oncology 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-b8d82c249a2cfd1f3ceb1461484b1233ccc396752d4211b91b9230ee2ae1abca3</citedby><cites>FETCH-LOGICAL-c469t-b8d82c249a2cfd1f3ceb1461484b1233ccc396752d4211b91b9230ee2ae1abca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1245/s10434-011-1591-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1245/s10434-011-1591-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21301968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yaguchi, Yoshihisa</creatorcontrib><creatorcontrib>Sugasawa, Hidekazu</creatorcontrib><creatorcontrib>Tsujimoto, Hironori</creatorcontrib><creatorcontrib>Takata, Hideki</creatorcontrib><creatorcontrib>Nakabayashi, Kadzuki</creatorcontrib><creatorcontrib>Ichikura, Takashi</creatorcontrib><creatorcontrib>Ono, Satoshi</creatorcontrib><creatorcontrib>Hiraki, Shuichi</creatorcontrib><creatorcontrib>Sakamoto, Naoko</creatorcontrib><creatorcontrib>Horio, Takuya</creatorcontrib><creatorcontrib>Kumano, Isao</creatorcontrib><creatorcontrib>Otomo, Yasuhiro</creatorcontrib><creatorcontrib>Mochizuki, Hidetaka</creatorcontrib><creatorcontrib>Yamamoto, Junji</creatorcontrib><creatorcontrib>Hase, Kazuo</creatorcontrib><title>One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer</title><title>Annals of surgical oncology</title><addtitle>Ann Surg Oncol</addtitle><addtitle>Ann Surg Oncol</addtitle><description>Purpose
This study was designed to apply safely the sentinel node navigation surgery (SNNS) to the malignancies, an accurate and prompt intraoperative diagnosis of SN is essential, and micrometastasis has been frequently missed by conventional frozen sections. Recently, a novel molecular-based rapid diagnosis for the lymph node (LN) metastases has been developed using (OSNA) in breast cancer, which takes approximately 30 min to obtain a final result. We evaluated the efficacy of OSNA in terms of the intraoperative diagnosis of LN metastasis in patients with gastric cancer.
Methods
A total of 162 LNs dissected from 32 patients with gastric cancer was included in this study; 45 LNs were pathologically diagnosed as metastatic LNs and 117 LNs were negative. The LNs were bisected; halves were examined with H&E stain, and the opposite halves were subjected to OSNA analyses of CK19 mRNA. The CK19 mRNA expression was examined in the positive or negative metastatic LNs, and the correlation between the tumor volume and CK19 mRNA expression in the metastatic LNs was examined.
Results
The CK19 mRNA expressions in the positive metastatic LNs were significantly higher than those of negative LNs. When 250 copies/μl was set as a cutoff value, the concordance rate was 94.4%, the sensitivity was 88.9%, and the specificity was 96.6%. The OSNA expression was significantly correlated with the estimated tumor volumes in the metastatic LNs.
Conclusions
The OSNA method is feasible and acceptable for detecting LN metastases in patients with gastric cancer. This should be applied for the intraoperative diagnosis in the SN-navigation surgery in gastric cancer.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gastrectomy</subject><subject>Gastrointestinal Oncology</subject><subject>Humans</subject><subject>Intestinal Neoplasms - diagnosis</subject><subject>Intestinal Neoplasms - surgery</subject><subject>Keratin-19 - genetics</subject><subject>Keratin-19 - metabolism</subject><subject>Lymph Nodes - pathology</subject><subject>Lymph Nodes - surgery</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Monitoring, Intraoperative</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Oncology</subject><subject>Prognosis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Sentinel Lymph Node Biopsy</subject><subject>Stomach Neoplasms - diagnosis</subject><subject>Stomach Neoplasms - surgery</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Survival Rate</subject><issn>1068-9265</issn><issn>1534-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUFrFDEUx4MotlY_gBcJXqyHqXlJJpMch0WrUHYPW89DJvNGU2aTMZk5-O2bsquC0EIg4b3f-z3Cn5C3wK6Ay_pTBiaFrBhABbWByjwj51CXilQanpc3U7oyXNVn5FXOd4xBI1j9kpxxEAyM0ufE7wJW-wVnul3dhN7R1vmBtod58qN3dvEx0Mvdftt-pGNMdPmJtJ1L89SKI91jWHzAiW7jgHQTg8N5oT7Qa5uXVIwbW0rpNXkx2injm9N9Qb5_-Xy7-Vrd7K6_bdqbyklllqrXg-aOS2O5GwcYhcMepAKpZQ9cCOecMKqp-SA5QG_K4YIhcotge2fFBflw9M4p_loxL93BZ4fTZAPGNXe6aSQIaWQhL58kgTGteS0UL-j7_9C7uKZQ_lF8qmGN0VAgOEIuxZwTjt2c_MGm38XUPQTWHQPrSmDdQ2CdKTPvTuK1P-Dwd-JPQgXgRyCXVviB6d_mx633EvWeRQ</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Yaguchi, Yoshihisa</creator><creator>Sugasawa, Hidekazu</creator><creator>Tsujimoto, Hironori</creator><creator>Takata, Hideki</creator><creator>Nakabayashi, Kadzuki</creator><creator>Ichikura, Takashi</creator><creator>Ono, Satoshi</creator><creator>Hiraki, Shuichi</creator><creator>Sakamoto, Naoko</creator><creator>Horio, Takuya</creator><creator>Kumano, Isao</creator><creator>Otomo, Yasuhiro</creator><creator>Mochizuki, Hidetaka</creator><creator>Yamamoto, Junji</creator><creator>Hase, Kazuo</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20110801</creationdate><title>One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer</title><author>Yaguchi, Yoshihisa ; Sugasawa, Hidekazu ; Tsujimoto, Hironori ; Takata, Hideki ; Nakabayashi, Kadzuki ; Ichikura, Takashi ; Ono, Satoshi ; Hiraki, Shuichi ; Sakamoto, Naoko ; Horio, Takuya ; Kumano, Isao ; Otomo, Yasuhiro ; Mochizuki, Hidetaka ; Yamamoto, Junji ; Hase, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-b8d82c249a2cfd1f3ceb1461484b1233ccc396752d4211b91b9230ee2ae1abca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Gastrectomy</topic><topic>Gastrointestinal Oncology</topic><topic>Humans</topic><topic>Intestinal Neoplasms - diagnosis</topic><topic>Intestinal Neoplasms - surgery</topic><topic>Keratin-19 - genetics</topic><topic>Keratin-19 - metabolism</topic><topic>Lymph Nodes - pathology</topic><topic>Lymph Nodes - surgery</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Monitoring, Intraoperative</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Oncology</topic><topic>Prognosis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Sentinel Lymph Node Biopsy</topic><topic>Stomach Neoplasms - diagnosis</topic><topic>Stomach Neoplasms - surgery</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Survival Rate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yaguchi, Yoshihisa</creatorcontrib><creatorcontrib>Sugasawa, Hidekazu</creatorcontrib><creatorcontrib>Tsujimoto, Hironori</creatorcontrib><creatorcontrib>Takata, Hideki</creatorcontrib><creatorcontrib>Nakabayashi, Kadzuki</creatorcontrib><creatorcontrib>Ichikura, Takashi</creatorcontrib><creatorcontrib>Ono, Satoshi</creatorcontrib><creatorcontrib>Hiraki, Shuichi</creatorcontrib><creatorcontrib>Sakamoto, Naoko</creatorcontrib><creatorcontrib>Horio, Takuya</creatorcontrib><creatorcontrib>Kumano, Isao</creatorcontrib><creatorcontrib>Otomo, Yasuhiro</creatorcontrib><creatorcontrib>Mochizuki, Hidetaka</creatorcontrib><creatorcontrib>Yamamoto, Junji</creatorcontrib><creatorcontrib>Hase, Kazuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yaguchi, Yoshihisa</au><au>Sugasawa, Hidekazu</au><au>Tsujimoto, Hironori</au><au>Takata, Hideki</au><au>Nakabayashi, Kadzuki</au><au>Ichikura, Takashi</au><au>Ono, Satoshi</au><au>Hiraki, Shuichi</au><au>Sakamoto, Naoko</au><au>Horio, Takuya</au><au>Kumano, Isao</au><au>Otomo, Yasuhiro</au><au>Mochizuki, Hidetaka</au><au>Yamamoto, Junji</au><au>Hase, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer</atitle><jtitle>Annals of surgical oncology</jtitle><stitle>Ann Surg Oncol</stitle><addtitle>Ann Surg Oncol</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>18</volume><issue>8</issue><spage>2289</spage><epage>2296</epage><pages>2289-2296</pages><issn>1068-9265</issn><eissn>1534-4681</eissn><abstract>Purpose
This study was designed to apply safely the sentinel node navigation surgery (SNNS) to the malignancies, an accurate and prompt intraoperative diagnosis of SN is essential, and micrometastasis has been frequently missed by conventional frozen sections. Recently, a novel molecular-based rapid diagnosis for the lymph node (LN) metastases has been developed using (OSNA) in breast cancer, which takes approximately 30 min to obtain a final result. We evaluated the efficacy of OSNA in terms of the intraoperative diagnosis of LN metastasis in patients with gastric cancer.
Methods
A total of 162 LNs dissected from 32 patients with gastric cancer was included in this study; 45 LNs were pathologically diagnosed as metastatic LNs and 117 LNs were negative. The LNs were bisected; halves were examined with H&E stain, and the opposite halves were subjected to OSNA analyses of CK19 mRNA. The CK19 mRNA expression was examined in the positive or negative metastatic LNs, and the correlation between the tumor volume and CK19 mRNA expression in the metastatic LNs was examined.
Results
The CK19 mRNA expressions in the positive metastatic LNs were significantly higher than those of negative LNs. When 250 copies/μl was set as a cutoff value, the concordance rate was 94.4%, the sensitivity was 88.9%, and the specificity was 96.6%. The OSNA expression was significantly correlated with the estimated tumor volumes in the metastatic LNs.
Conclusions
The OSNA method is feasible and acceptable for detecting LN metastases in patients with gastric cancer. This should be applied for the intraoperative diagnosis in the SN-navigation surgery in gastric cancer.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>21301968</pmid><doi>10.1245/s10434-011-1591-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1068-9265 |
| ispartof | Annals of surgical oncology, 2011-08, Vol.18 (8), p.2289-2296 |
| issn | 1068-9265 1534-4681 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_877413494 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Adult Aged Aged, 80 and over Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Blotting, Western Feasibility Studies Female Follow-Up Studies Gastrectomy Gastrointestinal Oncology Humans Intestinal Neoplasms - diagnosis Intestinal Neoplasms - surgery Keratin-19 - genetics Keratin-19 - metabolism Lymph Nodes - pathology Lymph Nodes - surgery Lymphatic Metastasis Male Medicine Medicine & Public Health Middle Aged Monitoring, Intraoperative Nucleic Acid Amplification Techniques Oncology Prognosis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Sentinel Lymph Node Biopsy Stomach Neoplasms - diagnosis Stomach Neoplasms - surgery Surgery Surgical Oncology Survival Rate |
| title | One-Step Nucleic Acid Amplification (OSNA) for the Application of Sentinel Node Concept in Gastric Cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T02%3A08%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=One-Step%20Nucleic%20Acid%20Amplification%20(OSNA)%20for%20the%20Application%20of%20Sentinel%20Node%20Concept%20in%20Gastric%20Cancer&rft.jtitle=Annals%20of%20surgical%20oncology&rft.au=Yaguchi,%20Yoshihisa&rft.date=2011-08-01&rft.volume=18&rft.issue=8&rft.spage=2289&rft.epage=2296&rft.pages=2289-2296&rft.issn=1068-9265&rft.eissn=1534-4681&rft_id=info:doi/10.1245/s10434-011-1591-9&rft_dat=%3Cproquest_cross%3E2399034011%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=876707981&rft_id=info:pmid/21301968&rfr_iscdi=true |