Down‐regulation of c‐Myc expression inhibits the invasion of bile duct carcinoma cells
Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c‐Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c‐Myc to drive unrestricted cell proliferation and to...
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| Veröffentlicht in: | Cell biology international 2011-08, Vol.35 (8), p.799-802 |
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| creator | Li, Zhuo‐Ri Wu, Yi‐Fei Ma, Chun‐Yang Nie, Shen‐Dan Mao, Xian‐Hai Shi, Yong‐Zhong |
| description | Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c‐Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c‐Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down‐regulated c‐Myc expression can inhibit tumour cell invasion still remains to be explored. The c‐Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c‐Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c‐Myc ASODN could significantly suppress the c‐Myc protein expression (P |
| doi_str_mv | 10.1042/CBI20110099 |
| format | Article |
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Enhanced c‐Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c‐Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down‐regulated c‐Myc expression can inhibit tumour cell invasion still remains to be explored. The c‐Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c‐Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c‐Myc ASODN could significantly suppress the c‐Myc protein expression (P<0.05) and the invasion (P<0.01) of QBC939 cells transfected with c‐Myc ASODN compared with that in the control and c‐Myc NSODN‐transfected group. Thus in the present study we show that down‐regulation of c‐Myc expression can inhibit the invasion of QBC939 cells in vitro.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1042/CBI20110099</identifier><identifier>PMID: 21557726</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bile Duct Neoplasms - genetics ; Bile Duct Neoplasms - metabolism ; Bile Duct Neoplasms - pathology ; Bile Ducts, Intrahepatic - pathology ; Cell Line, Tumor ; Cell Proliferation - drug effects ; cholangiocarcinoma ; Cholangiocarcinoma - genetics ; Cholangiocarcinoma - metabolism ; Cholangiocarcinoma - pathology ; Codon, Nonsense - genetics ; Codon, Nonsense - pharmacology ; c‐Myc ; Down-Regulation - drug effects ; Gene Expression Regulation, Neoplastic ; Humans ; invasion ; Liver - pathology ; Liver Neoplasms - metabolism ; Neoplasm Invasiveness ; Oligodeoxyribonucleotides, Antisense - genetics ; Oligodeoxyribonucleotides, Antisense - pharmacology ; Proto-Oncogene Proteins c-myc - biosynthesis ; Proto-Oncogene Proteins c-myc - genetics ; Transfection</subject><ispartof>Cell biology international, 2011-08, Vol.35 (8), p.799-802</ispartof><rights>The Author(s) Journal compilation © 2011 International Federation for Cell Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3308-b938e69297ffbbc1f570e3d1912f223aa65b7b9d7017f6eb82b06e6b7bfadd43</citedby><cites>FETCH-LOGICAL-c3308-b938e69297ffbbc1f570e3d1912f223aa65b7b9d7017f6eb82b06e6b7bfadd43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1042%2FCBI20110099$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1042%2FCBI20110099$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21557726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zhuo‐Ri</creatorcontrib><creatorcontrib>Wu, Yi‐Fei</creatorcontrib><creatorcontrib>Ma, Chun‐Yang</creatorcontrib><creatorcontrib>Nie, Shen‐Dan</creatorcontrib><creatorcontrib>Mao, Xian‐Hai</creatorcontrib><creatorcontrib>Shi, Yong‐Zhong</creatorcontrib><title>Down‐regulation of c‐Myc expression inhibits the invasion of bile duct carcinoma cells</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c‐Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c‐Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down‐regulated c‐Myc expression can inhibit tumour cell invasion still remains to be explored. The c‐Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c‐Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c‐Myc ASODN could significantly suppress the c‐Myc protein expression (P<0.05) and the invasion (P<0.01) of QBC939 cells transfected with c‐Myc ASODN compared with that in the control and c‐Myc NSODN‐transfected group. Thus in the present study we show that down‐regulation of c‐Myc expression can inhibit the invasion of QBC939 cells in vitro.</description><subject>Bile Duct Neoplasms - genetics</subject><subject>Bile Duct Neoplasms - metabolism</subject><subject>Bile Duct Neoplasms - pathology</subject><subject>Bile Ducts, Intrahepatic - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>cholangiocarcinoma</subject><subject>Cholangiocarcinoma - genetics</subject><subject>Cholangiocarcinoma - metabolism</subject><subject>Cholangiocarcinoma - pathology</subject><subject>Codon, Nonsense - genetics</subject><subject>Codon, Nonsense - pharmacology</subject><subject>c‐Myc</subject><subject>Down-Regulation - drug effects</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>invasion</subject><subject>Liver - pathology</subject><subject>Liver Neoplasms - metabolism</subject><subject>Neoplasm Invasiveness</subject><subject>Oligodeoxyribonucleotides, Antisense - genetics</subject><subject>Oligodeoxyribonucleotides, Antisense - pharmacology</subject><subject>Proto-Oncogene Proteins c-myc - biosynthesis</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Transfection</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLlOAzEURS0EIiFQ0aPpKNCAl3grIWyRWJpUNCPbYxOjWcJ4hpCOT-Ab-RI8SkBUVO--q6Or9y4AhwieIjjGZ5OLKYYIQSjlFhgiKGkqCKXbvWY0ZVLSAdgL4QVGaizYLhhgRCnnmA3B02W9rL4-Phv73BWq9XWV1C4x0blfmcS-LxobQu_6au61b0PSzm1c3lTYsNoXNsk70yZGNcZXdakSY4si7IMdp4pgDzZzBGbXV7PJbXr3eDOdnN-lhhAoUi2JsExiyZ3T2iBHObQkRxJhhzFRilHNtcw5RNwxqwXWkFkWPafyfExG4Hgdu2jq186GNit96A9Qla27kAnORPyawUierEnT1CE01mWLxpeqWWUIZn2V2Z8qI320ye10afNf9qe7CKA1sIwNrP7L6vUDhlKQbyVLf3s</recordid><startdate>201108</startdate><enddate>201108</enddate><creator>Li, Zhuo‐Ri</creator><creator>Wu, Yi‐Fei</creator><creator>Ma, Chun‐Yang</creator><creator>Nie, Shen‐Dan</creator><creator>Mao, Xian‐Hai</creator><creator>Shi, Yong‐Zhong</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201108</creationdate><title>Down‐regulation of c‐Myc expression inhibits the invasion of bile duct carcinoma cells</title><author>Li, Zhuo‐Ri ; Wu, Yi‐Fei ; Ma, Chun‐Yang ; Nie, Shen‐Dan ; Mao, Xian‐Hai ; Shi, Yong‐Zhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3308-b938e69297ffbbc1f570e3d1912f223aa65b7b9d7017f6eb82b06e6b7bfadd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bile Duct Neoplasms - genetics</topic><topic>Bile Duct Neoplasms - metabolism</topic><topic>Bile Duct Neoplasms - pathology</topic><topic>Bile Ducts, Intrahepatic - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>cholangiocarcinoma</topic><topic>Cholangiocarcinoma - genetics</topic><topic>Cholangiocarcinoma - metabolism</topic><topic>Cholangiocarcinoma - pathology</topic><topic>Codon, Nonsense - genetics</topic><topic>Codon, Nonsense - pharmacology</topic><topic>c‐Myc</topic><topic>Down-Regulation - drug effects</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>invasion</topic><topic>Liver - pathology</topic><topic>Liver Neoplasms - metabolism</topic><topic>Neoplasm Invasiveness</topic><topic>Oligodeoxyribonucleotides, Antisense - genetics</topic><topic>Oligodeoxyribonucleotides, Antisense - pharmacology</topic><topic>Proto-Oncogene Proteins c-myc - biosynthesis</topic><topic>Proto-Oncogene Proteins c-myc - genetics</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhuo‐Ri</creatorcontrib><creatorcontrib>Wu, Yi‐Fei</creatorcontrib><creatorcontrib>Ma, Chun‐Yang</creatorcontrib><creatorcontrib>Nie, Shen‐Dan</creatorcontrib><creatorcontrib>Mao, Xian‐Hai</creatorcontrib><creatorcontrib>Shi, Yong‐Zhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhuo‐Ri</au><au>Wu, Yi‐Fei</au><au>Ma, Chun‐Yang</au><au>Nie, Shen‐Dan</au><au>Mao, Xian‐Hai</au><au>Shi, Yong‐Zhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down‐regulation of c‐Myc expression inhibits the invasion of bile duct carcinoma cells</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2011-08</date><risdate>2011</risdate><volume>35</volume><issue>8</issue><spage>799</spage><epage>802</epage><pages>799-802</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c‐Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c‐Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down‐regulated c‐Myc expression can inhibit tumour cell invasion still remains to be explored. The c‐Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c‐Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c‐Myc ASODN could significantly suppress the c‐Myc protein expression (P<0.05) and the invasion (P<0.01) of QBC939 cells transfected with c‐Myc ASODN compared with that in the control and c‐Myc NSODN‐transfected group. Thus in the present study we show that down‐regulation of c‐Myc expression can inhibit the invasion of QBC939 cells in vitro.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21557726</pmid><doi>10.1042/CBI20110099</doi><tpages>4</tpages></addata></record> |
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| subjects | Bile Duct Neoplasms - genetics Bile Duct Neoplasms - metabolism Bile Duct Neoplasms - pathology Bile Ducts, Intrahepatic - pathology Cell Line, Tumor Cell Proliferation - drug effects cholangiocarcinoma Cholangiocarcinoma - genetics Cholangiocarcinoma - metabolism Cholangiocarcinoma - pathology Codon, Nonsense - genetics Codon, Nonsense - pharmacology c‐Myc Down-Regulation - drug effects Gene Expression Regulation, Neoplastic Humans invasion Liver - pathology Liver Neoplasms - metabolism Neoplasm Invasiveness Oligodeoxyribonucleotides, Antisense - genetics Oligodeoxyribonucleotides, Antisense - pharmacology Proto-Oncogene Proteins c-myc - biosynthesis Proto-Oncogene Proteins c-myc - genetics Transfection |
| title | Down‐regulation of c‐Myc expression inhibits the invasion of bile duct carcinoma cells |
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