New Highly Sensitive and Accurate Lyophilized Real-Time RT-PCR Tests for Early Detection of Avian Influenza

New lyophilized real-time reverse transcription (RT)-PCR avian influenza detection assays were designed and tested. The M-gene assay detects all avian influenza virus (AIV) subtypes, and the H5 and H7 specific assays can discriminate the AIV subtypes H5 and H7 of Eurasian origin. The assays are form...

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Veröffentlicht in:	Avian diseases 2010-03, Vol.54 (s1), p.686-689
Hauptverfasser:	Petrauskene, Olga V, Schumaker, Michael A, Thorstenson, Yvonne, Fearnley, Catherine, Pavlidis, Theo, Liew, Sueh-Ning, Cork, Jennifer, Furtado, Manohar R, Wakeley, Philip R, Slomka, Marek J
Format:	Artikel
Sprache:	eng
Schlagworte:	21st Century Diagnostics for Centuries Old Problem accuracy Animals Avian influenza virus Chick Embryo diagnostic techniques disease detection disease diagnosis Freeze Drying H5 H5N1 subtype influenza A virus H5N2 subtype influenza A virus H7 hemagglutinins highly pathogenic avian influenza H5N1 Influenza A virus Influenza in Birds - diagnosis laboratory techniques lyophilized tests M-gene methodology new methods Notifiable diseases pathogen identification pathogenesis Pathogens real-time RT-PCR Reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA Sensitivity and Specificity serotypes strain differences test sensitivity test specificity Turkeys vertebrate viruses Veterinary medicine Viruses Wild birds
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