Efficient gene delivery into fish cells by an improved recombinant baculovirus
Studies on transduction of mammalian cells have shown that baculovirus can be used as an effective vector for gene delivery. However, previous studies have found the gene delivery efficiency to be very low in differentiated fish cells. In this study, an improved recombinant baculovirus, containing c...
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| Veröffentlicht in: | Journal of virological methods 2011-05, Vol.173 (2), p.294-299 |
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| creator | Huang, Fengtao Cao, Shengbo Cui, Xiaoxian Xiong, Chuanxi Wang, Min Lu, Yuanan Wang, Weimin Ye, Jing Liu, Xueqin |
| description | Studies on transduction of mammalian cells have shown that baculovirus can be used as an effective vector for gene delivery. However, previous studies have found the gene delivery efficiency to be very low in differentiated fish cells. In this study, an improved recombinant baculovirus, containing cytomegalovirus immediate-early (CMV-IE) promoter and an enhanced green fluorescent protein (EGFP) gene as the reporter gene, was constructed. The transduction efficiency of recombinant baculovirus in several fish cell lines was measured by flow cytometry (FCM) and the persistence of EGFP was monitored by fluorescence microscopy. The results demonstrated that baculovirus can mediate the high efficiency of gene delivery into differentiated fish cells. Furthermore, it was found that growth medium is superior to PBS as the surrounding solution to enhance the transduction efficiency in some fish cells. In addition, transgene expression can persist for a lengthy period in fish cells, and attained highest level several days later after transduction. This study suggest that the improved recombinant baculovirus can be an excellent gene delivery vector for fish cells and also providing new insights into the transduction of vertebrate cells with baculovirus. |
| doi_str_mv | 10.1016/j.jviromet.2011.02.022 |
| format | Article |
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However, previous studies have found the gene delivery efficiency to be very low in differentiated fish cells. In this study, an improved recombinant baculovirus, containing cytomegalovirus immediate-early (CMV-IE) promoter and an enhanced green fluorescent protein (EGFP) gene as the reporter gene, was constructed. The transduction efficiency of recombinant baculovirus in several fish cell lines was measured by flow cytometry (FCM) and the persistence of EGFP was monitored by fluorescence microscopy. The results demonstrated that baculovirus can mediate the high efficiency of gene delivery into differentiated fish cells. Furthermore, it was found that growth medium is superior to PBS as the surrounding solution to enhance the transduction efficiency in some fish cells. In addition, transgene expression can persist for a lengthy period in fish cells, and attained highest level several days later after transduction. This study suggest that the improved recombinant baculovirus can be an excellent gene delivery vector for fish cells and also providing new insights into the transduction of vertebrate cells with baculovirus.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2011.02.022</identifier><identifier>PMID: 21354209</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Animals ; Baculoviridae ; Baculoviridae - genetics ; Baculovirus ; Baculovirus transduction ; Biological and medical sciences ; Biotechnology ; Cell Line ; culture media ; Culture Media - chemistry ; Cytomegalovirus ; Cytomegalovirus - genetics ; fish ; Fish cell ; Fishes ; Flow Cytometry ; fluorescence microscopy ; Fundamental and applied biological sciences. Psychology ; Gene delivery ; gene expression ; gene transfer ; Gene Transfer, Horizontal ; Genes, Reporter ; Genetic engineering ; Genetic technics ; Genetic Vectors ; green fluorescent protein ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; mammals ; Methods. Procedures. Technologies ; Microbiology ; Promoter Regions, Genetic ; reporter genes ; Techniques used in virology ; Transduction, Genetic - methods ; Vectors (cloning, transfer, expression). Insertion sequences and transposons ; Virology</subject><ispartof>Journal of virological methods, 2011-05, Vol.173 (2), p.294-299</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-d4df5c6de20174edf1e260d63c6f2b3a4c5043bf095a9c2213f59a31cd2baf1b3</citedby><cites>FETCH-LOGICAL-c453t-d4df5c6de20174edf1e260d63c6f2b3a4c5043bf095a9c2213f59a31cd2baf1b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jviromet.2011.02.022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24177228$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21354209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Fengtao</creatorcontrib><creatorcontrib>Cao, Shengbo</creatorcontrib><creatorcontrib>Cui, Xiaoxian</creatorcontrib><creatorcontrib>Xiong, Chuanxi</creatorcontrib><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Lu, Yuanan</creatorcontrib><creatorcontrib>Wang, Weimin</creatorcontrib><creatorcontrib>Ye, Jing</creatorcontrib><creatorcontrib>Liu, Xueqin</creatorcontrib><title>Efficient gene delivery into fish cells by an improved recombinant baculovirus</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>Studies on transduction of mammalian cells have shown that baculovirus can be used as an effective vector for gene delivery. However, previous studies have found the gene delivery efficiency to be very low in differentiated fish cells. In this study, an improved recombinant baculovirus, containing cytomegalovirus immediate-early (CMV-IE) promoter and an enhanced green fluorescent protein (EGFP) gene as the reporter gene, was constructed. The transduction efficiency of recombinant baculovirus in several fish cell lines was measured by flow cytometry (FCM) and the persistence of EGFP was monitored by fluorescence microscopy. The results demonstrated that baculovirus can mediate the high efficiency of gene delivery into differentiated fish cells. Furthermore, it was found that growth medium is superior to PBS as the surrounding solution to enhance the transduction efficiency in some fish cells. In addition, transgene expression can persist for a lengthy period in fish cells, and attained highest level several days later after transduction. This study suggest that the improved recombinant baculovirus can be an excellent gene delivery vector for fish cells and also providing new insights into the transduction of vertebrate cells with baculovirus.</description><subject>Animals</subject><subject>Baculoviridae</subject><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>Baculovirus transduction</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>culture media</subject><subject>Culture Media - chemistry</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - genetics</subject><subject>fish</subject><subject>Fish cell</subject><subject>Fishes</subject><subject>Flow Cytometry</subject><subject>fluorescence microscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene delivery</subject><subject>gene expression</subject><subject>gene transfer</subject><subject>Gene Transfer, Horizontal</subject><subject>Genes, Reporter</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>green fluorescent protein</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>mammals</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Promoter Regions, Genetic</subject><subject>reporter genes</subject><subject>Techniques used in virology</subject><subject>Transduction, Genetic - methods</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EokPhLxRvEKsZ_E6yA1XlIVWwgK4tx74uHiVxsZOR5t9zo5nCstKVrhff8T06h5ArznaccfNhv9sfUskjzDvBON8xgSOekQ1vm27LulY9JxsEDb6luiCvat0zxnQj5UtyIbjUSrBuQ77fxJh8gmmm9zABDTCkA5QjTdOcaUz1N_UwDJX2R-ommsaHkg8QaAGfxz5NDoW988uQ0c5SX5MX0Q0V3pz3Jbn7fPPr-uv29seXb9efbrdeaTlvgwpRexMAvTcKQuQgDAtGehNFL53yminZR9Zp13mBdqPunOQ-iN5F3stL8v70L9r5s0Cd7ZjqatRNkJdq28YIpVXTPE0avMxaLpA0J9KXXGuBaB9KGl05Ws7sGrrd28fQ7Rq6ZQJnFV6dTyz9COGf7DFlBN6dAVe9G2Jxk0_1P6d40wjRIvf2xEWXrbsvyNz9xEtmba6VWiPx8UQAhntIUGxdy_MQEjYy25DTU27_ArzerT4</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Huang, Fengtao</creator><creator>Cao, Shengbo</creator><creator>Cui, Xiaoxian</creator><creator>Xiong, Chuanxi</creator><creator>Wang, Min</creator><creator>Lu, Yuanan</creator><creator>Wang, Weimin</creator><creator>Ye, Jing</creator><creator>Liu, Xueqin</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20110501</creationdate><title>Efficient gene delivery into fish cells by an improved recombinant baculovirus</title><author>Huang, Fengtao ; Cao, Shengbo ; Cui, Xiaoxian ; Xiong, Chuanxi ; Wang, Min ; Lu, Yuanan ; Wang, Weimin ; Ye, Jing ; Liu, Xueqin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-d4df5c6de20174edf1e260d63c6f2b3a4c5043bf095a9c2213f59a31cd2baf1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Baculoviridae</topic><topic>Baculoviridae - genetics</topic><topic>Baculovirus</topic><topic>Baculovirus transduction</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>culture media</topic><topic>Culture Media - chemistry</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus - genetics</topic><topic>fish</topic><topic>Fish cell</topic><topic>Fishes</topic><topic>Flow Cytometry</topic><topic>fluorescence microscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene delivery</topic><topic>gene expression</topic><topic>gene transfer</topic><topic>Gene Transfer, Horizontal</topic><topic>Genes, Reporter</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>green fluorescent protein</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>mammals</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Promoter Regions, Genetic</topic><topic>reporter genes</topic><topic>Techniques used in virology</topic><topic>Transduction, Genetic - methods</topic><topic>Vectors (cloning, transfer, expression). 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However, previous studies have found the gene delivery efficiency to be very low in differentiated fish cells. In this study, an improved recombinant baculovirus, containing cytomegalovirus immediate-early (CMV-IE) promoter and an enhanced green fluorescent protein (EGFP) gene as the reporter gene, was constructed. The transduction efficiency of recombinant baculovirus in several fish cell lines was measured by flow cytometry (FCM) and the persistence of EGFP was monitored by fluorescence microscopy. The results demonstrated that baculovirus can mediate the high efficiency of gene delivery into differentiated fish cells. Furthermore, it was found that growth medium is superior to PBS as the surrounding solution to enhance the transduction efficiency in some fish cells. In addition, transgene expression can persist for a lengthy period in fish cells, and attained highest level several days later after transduction. This study suggest that the improved recombinant baculovirus can be an excellent gene delivery vector for fish cells and also providing new insights into the transduction of vertebrate cells with baculovirus.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>21354209</pmid><doi>10.1016/j.jviromet.2011.02.022</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Baculoviridae Baculoviridae - genetics Baculovirus Baculovirus transduction Biological and medical sciences Biotechnology Cell Line culture media Culture Media - chemistry Cytomegalovirus Cytomegalovirus - genetics fish Fish cell Fishes Flow Cytometry fluorescence microscopy Fundamental and applied biological sciences. Psychology Gene delivery gene expression gene transfer Gene Transfer, Horizontal Genes, Reporter Genetic engineering Genetic technics Genetic Vectors green fluorescent protein Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism mammals Methods. Procedures. Technologies Microbiology Promoter Regions, Genetic reporter genes Techniques used in virology Transduction, Genetic - methods Vectors (cloning, transfer, expression). Insertion sequences and transposons Virology |
| title | Efficient gene delivery into fish cells by an improved recombinant baculovirus |
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