Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis

Abstract The feasibility of using high-resolution melting analysis (HRMA) was examined for its rapid and simple detection of 5 clinically relevant Candida species ( C. albicans , C. glabrata , C. kefyr , C. parapsilosis , and C. guilliermondii ). HRMA was able to differentiate clinical Candida speci...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2011-03, Vol.69 (3), p.283-285
Hauptverfasser:	Arancia, Silvia, Sandini, Silvia, De Bernardis, Flavia, Fortini, Daniela
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Candida Candida - classification Candida - genetics Candida spp Candidiasis - diagnosis Candidiasis - microbiology DNA, Fungal - chemistry Early diagnosis Fundamental and applied biological sciences. Psychology High-resolution melting analysis (HRMA) Humans Infectious Disease Infectious diseases Internal Medicine Medical sciences Microbiology Miscellaneous MP65 Mycological Typing Techniques - economics Mycological Typing Techniques - methods Mycology Nucleic Acid Denaturation Polymerase Chain Reaction Reproducibility of Results Sensitivity and Specificity Sequence Analysis, DNA - methods
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Zusammenfassung:	Abstract The feasibility of using high-resolution melting analysis (HRMA) was examined for its rapid and simple detection of 5 clinically relevant Candida species ( C. albicans , C. glabrata , C. kefyr , C. parapsilosis , and C. guilliermondii ). HRMA was able to differentiate clinical Candida species and resulted in being sensitive, reproducible, and inexpensive.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2010.10.003