Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid
We present a novel immunoassay format utilizing the catalytic properties of gold nanoparticles in the luminol-silver nitrate-gold nanoparticle based chemiluminescence (CL) system for the detection of widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Highly sensitive anti-2,4-D antibody w...
Gespeichert in:
| Veröffentlicht in: | Analyst (London) 2011-05, Vol.136 (10), p.2125-2130 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2130 |
|---|---|
| container_issue | 10 |
| container_start_page | 2125 |
| container_title | Analyst (London) |
| container_volume | 136 |
| creator | ROBIN CHANDRA BORO KAUSHAL, Jyotsna NANGIA, Yogesh WANGOO, Nishima BHASIN, Aman RAMAN SURF, C |
| description | We present a novel immunoassay format utilizing the catalytic properties of gold nanoparticles in the luminol-silver nitrate-gold nanoparticle based chemiluminescence (CL) system for the detection of widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Highly sensitive anti-2,4-D antibody was produced and conjugated with gold nanoparticles of various sizes. In the present assay format, employing a competitive inhibition approach, a well-characterized hapten-protein conjugate (2,4-D-BSA) was used to coat the microtiter plates. The analyte (2,4-D) was pre-incubated with anti-2,4-D antibody labeled with gold nanoparticles and added to each well of the microtiter plate. The gold label triggered the reaction between luminol and silver nitrate generating a luminescence signal at 425 nm. Under the optimized conditions, the CL based immunoassay showed the detection limit of 2,4-D in standard water samples around 3 ng mL(-1). The CL based immunoassay format, based on gold nanoparticles as a catalyst, could be used as a fast screening methodology ( |
| doi_str_mv | 10.1039/c0an00810a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_876236910</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>863900404</sourcerecordid><originalsourceid>FETCH-LOGICAL-c348t-6805844e9628abe8144b01684bb6250656d4f50883a88c6ea3fc649bed839843</originalsourceid><addsrcrecordid>eNqFkU1r3DAQhkVpaDbbXvoDii6lEOJ0ZH2sfAyh-YBALrmbsTxmVWRpK9nQza-Pw26bY07DMA8PzPsy9lXApQDZ_HSAEcAKwA9sJaRRlda1_chWACCr2mh1ys5K-b2sAjR8Yqe1UFprKVdsuk2h5xFj2mGevAtUuMMJw_6Zeu62NPowjz5ScRQdcT-Oc0xYCu75kDLvaSI3-RR5GviWcued74nXF6rqvduGlNNuSzH93aOjxc9xuX9mJwOGQl-Oc82ebn49Xd9VD4-399dXD5WTyk6VsaCtUtSY2mJHVijVgTBWdZ2pNRhtejVosFaitc4QysEZ1XTUW9lYJdfsx0G7y-nPTGVqR7-8EQJGSnNp7cbU0jRLhu-SRjYACl6d5wfS5VRKpqHdZT9i3rcC2tc22rc2FvjbUTt3I_X_0X_xL8D3I4DFYRgyRufLG6fEZiOFlC-HMJMB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>863900404</pqid></control><display><type>article</type><title>Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid</title><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>ROBIN CHANDRA BORO ; KAUSHAL, Jyotsna ; NANGIA, Yogesh ; WANGOO, Nishima ; BHASIN, Aman ; RAMAN SURF, C</creator><creatorcontrib>ROBIN CHANDRA BORO ; KAUSHAL, Jyotsna ; NANGIA, Yogesh ; WANGOO, Nishima ; BHASIN, Aman ; RAMAN SURF, C</creatorcontrib><description>We present a novel immunoassay format utilizing the catalytic properties of gold nanoparticles in the luminol-silver nitrate-gold nanoparticle based chemiluminescence (CL) system for the detection of widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Highly sensitive anti-2,4-D antibody was produced and conjugated with gold nanoparticles of various sizes. In the present assay format, employing a competitive inhibition approach, a well-characterized hapten-protein conjugate (2,4-D-BSA) was used to coat the microtiter plates. The analyte (2,4-D) was pre-incubated with anti-2,4-D antibody labeled with gold nanoparticles and added to each well of the microtiter plate. The gold label triggered the reaction between luminol and silver nitrate generating a luminescence signal at 425 nm. Under the optimized conditions, the CL based immunoassay showed the detection limit of 2,4-D in standard water samples around 3 ng mL(-1). The CL based immunoassay format, based on gold nanoparticles as a catalyst, could be used as a fast screening methodology (<30 min) for pesticide detection.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c0an00810a</identifier><identifier>PMID: 21455533</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Analytical chemistry ; Chemical and thermal methods ; Chemistry ; Exact sciences and technology ; Miscellaneous ; Spectrometric and optical methods</subject><ispartof>Analyst (London), 2011-05, Vol.136 (10), p.2125-2130</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-6805844e9628abe8144b01684bb6250656d4f50883a88c6ea3fc649bed839843</citedby><cites>FETCH-LOGICAL-c348t-6805844e9628abe8144b01684bb6250656d4f50883a88c6ea3fc649bed839843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2818,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24177313$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21455533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ROBIN CHANDRA BORO</creatorcontrib><creatorcontrib>KAUSHAL, Jyotsna</creatorcontrib><creatorcontrib>NANGIA, Yogesh</creatorcontrib><creatorcontrib>WANGOO, Nishima</creatorcontrib><creatorcontrib>BHASIN, Aman</creatorcontrib><creatorcontrib>RAMAN SURF, C</creatorcontrib><title>Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>We present a novel immunoassay format utilizing the catalytic properties of gold nanoparticles in the luminol-silver nitrate-gold nanoparticle based chemiluminescence (CL) system for the detection of widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Highly sensitive anti-2,4-D antibody was produced and conjugated with gold nanoparticles of various sizes. In the present assay format, employing a competitive inhibition approach, a well-characterized hapten-protein conjugate (2,4-D-BSA) was used to coat the microtiter plates. The analyte (2,4-D) was pre-incubated with anti-2,4-D antibody labeled with gold nanoparticles and added to each well of the microtiter plate. The gold label triggered the reaction between luminol and silver nitrate generating a luminescence signal at 425 nm. Under the optimized conditions, the CL based immunoassay showed the detection limit of 2,4-D in standard water samples around 3 ng mL(-1). The CL based immunoassay format, based on gold nanoparticles as a catalyst, could be used as a fast screening methodology (<30 min) for pesticide detection.</description><subject>Analytical chemistry</subject><subject>Chemical and thermal methods</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Miscellaneous</subject><subject>Spectrometric and optical methods</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkU1r3DAQhkVpaDbbXvoDii6lEOJ0ZH2sfAyh-YBALrmbsTxmVWRpK9nQza-Pw26bY07DMA8PzPsy9lXApQDZ_HSAEcAKwA9sJaRRlda1_chWACCr2mh1ys5K-b2sAjR8Yqe1UFprKVdsuk2h5xFj2mGevAtUuMMJw_6Zeu62NPowjz5ScRQdcT-Oc0xYCu75kDLvaSI3-RR5GviWcued74nXF6rqvduGlNNuSzH93aOjxc9xuX9mJwOGQl-Oc82ebn49Xd9VD4-399dXD5WTyk6VsaCtUtSY2mJHVijVgTBWdZ2pNRhtejVosFaitc4QysEZ1XTUW9lYJdfsx0G7y-nPTGVqR7-8EQJGSnNp7cbU0jRLhu-SRjYACl6d5wfS5VRKpqHdZT9i3rcC2tc22rc2FvjbUTt3I_X_0X_xL8D3I4DFYRgyRufLG6fEZiOFlC-HMJMB</recordid><startdate>20110521</startdate><enddate>20110521</enddate><creator>ROBIN CHANDRA BORO</creator><creator>KAUSHAL, Jyotsna</creator><creator>NANGIA, Yogesh</creator><creator>WANGOO, Nishima</creator><creator>BHASIN, Aman</creator><creator>RAMAN SURF, C</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TV</scope><scope>C1K</scope></search><sort><creationdate>20110521</creationdate><title>Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid</title><author>ROBIN CHANDRA BORO ; KAUSHAL, Jyotsna ; NANGIA, Yogesh ; WANGOO, Nishima ; BHASIN, Aman ; RAMAN SURF, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-6805844e9628abe8144b01684bb6250656d4f50883a88c6ea3fc649bed839843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical chemistry</topic><topic>Chemical and thermal methods</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Miscellaneous</topic><topic>Spectrometric and optical methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROBIN CHANDRA BORO</creatorcontrib><creatorcontrib>KAUSHAL, Jyotsna</creatorcontrib><creatorcontrib>NANGIA, Yogesh</creatorcontrib><creatorcontrib>WANGOO, Nishima</creatorcontrib><creatorcontrib>BHASIN, Aman</creatorcontrib><creatorcontrib>RAMAN SURF, C</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Pollution Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROBIN CHANDRA BORO</au><au>KAUSHAL, Jyotsna</au><au>NANGIA, Yogesh</au><au>WANGOO, Nishima</au><au>BHASIN, Aman</au><au>RAMAN SURF, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2011-05-21</date><risdate>2011</risdate><volume>136</volume><issue>10</issue><spage>2125</spage><epage>2130</epage><pages>2125-2130</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><coden>ANALAO</coden><abstract>We present a novel immunoassay format utilizing the catalytic properties of gold nanoparticles in the luminol-silver nitrate-gold nanoparticle based chemiluminescence (CL) system for the detection of widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Highly sensitive anti-2,4-D antibody was produced and conjugated with gold nanoparticles of various sizes. In the present assay format, employing a competitive inhibition approach, a well-characterized hapten-protein conjugate (2,4-D-BSA) was used to coat the microtiter plates. The analyte (2,4-D) was pre-incubated with anti-2,4-D antibody labeled with gold nanoparticles and added to each well of the microtiter plate. The gold label triggered the reaction between luminol and silver nitrate generating a luminescence signal at 425 nm. Under the optimized conditions, the CL based immunoassay showed the detection limit of 2,4-D in standard water samples around 3 ng mL(-1). The CL based immunoassay format, based on gold nanoparticles as a catalyst, could be used as a fast screening methodology (<30 min) for pesticide detection.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>21455533</pmid><doi>10.1039/c0an00810a</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2011-05, Vol.136 (10), p.2125-2130 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_876236910 |
| source | Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analytical chemistry Chemical and thermal methods Chemistry Exact sciences and technology Miscellaneous Spectrometric and optical methods |
| title | Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T08%3A22%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gold%20nanoparticles%20catalyzed%20chemiluminescence%20immunoassay%20for%20detection%20of%20herbicide%202,4-dichlorophenoxyacetic%20acid&rft.jtitle=Analyst%20(London)&rft.au=ROBIN%20CHANDRA%20BORO&rft.date=2011-05-21&rft.volume=136&rft.issue=10&rft.spage=2125&rft.epage=2130&rft.pages=2125-2130&rft.issn=0003-2654&rft.eissn=1364-5528&rft.coden=ANALAO&rft_id=info:doi/10.1039/c0an00810a&rft_dat=%3Cproquest_cross%3E863900404%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=863900404&rft_id=info:pmid/21455533&rfr_iscdi=true |